FOLT1_CAEEL
ID FOLT1_CAEEL Reviewed; 453 AA.
AC Q17766; A3FMP6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 3.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Folate transporter 1;
GN Name=folt-1; Synonyms=tag-330; ORFNames=C06H2.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=17475669; DOI=10.1152/ajpcell.00516.2006;
RA Balamurugan K., Ashokkumar B., Moussaif M., Sze J.Y., Said H.M.;
RT "Cloning and functional characterization of a folate transporter from the
RT nematode Caenorhabditis elegans.";
RL Am. J. Physiol. 293:C670-C681(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 267-395.
RX PubMed=12679813; DOI=10.1038/ng1140;
RA Reboul J., Vaglio P., Rual J.F., Lamesch P., Martinez M., Armstrong C.M.,
RA Li S., Jacotot L., Bertin N., Janky R., Moore T., Hudson J.R. Jr.,
RA Hartley J.L., Brasch M.A., Vandenhaute J., Boulton S., Endress G.A.,
RA Jenna S., Chevet E., Papasotiropoulos V., Tolias P.P., Ptacek J.,
RA Snyder M., Huang R., Chance M.R., Lee H., Doucette-Stamm L., Hill D.E.,
RA Vidal M.;
RT "C. elegans ORFeome version 1.1: experimental verification of the genome
RT annotation and resource for proteome-scale protein expression.";
RL Nat. Genet. 34:35-41(2003).
CC -!- FUNCTION: Folate transporter. {ECO:0000269|PubMed:17475669}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in pharynx and posterior part of
CC the intestine. Expressed at lower levels in the body wall muscles, head
CC muscles, and vulva muscles. Highly expressed in the intestine of the
CC early larva, levels decrease in the later stages of development.
CC {ECO:0000269|PubMed:17475669}.
CC -!- DISRUPTION PHENOTYPE: Worms display defects in folate uptake,
CC reproduction and movement. {ECO:0000269|PubMed:17475669}.
CC -!- SIMILARITY: Belongs to the reduced folate carrier (RFC) transporter (TC
CC 2.A.48) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CB397828; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; EF409995; ABN58742.1; -; mRNA.
DR EMBL; Z75526; CAA99770.3; -; Genomic_DNA.
DR EMBL; CB397828; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T19024; T19024.
DR RefSeq; NP_505833.3; NM_073432.3.
DR AlphaFoldDB; Q17766; -.
DR STRING; 6239.C06H2.4; -.
DR TCDB; 2.A.48.1.3; the reduced folate carrier (rfc) family.
DR EnsemblMetazoa; C06H2.4.1; C06H2.4.1; WBGene00007388.
DR GeneID; 179544; -.
DR KEGG; cel:CELE_C06H2.4; -.
DR UCSC; C06H2.4; c. elegans.
DR CTD; 179544; -.
DR WormBase; C06H2.4; CE47283; WBGene00007388; folt-1.
DR eggNOG; KOG3810; Eukaryota.
DR GeneTree; ENSGT00950000183022; -.
DR HOGENOM; CLU_036909_0_1_1; -.
DR InParanoid; Q17766; -.
DR OMA; VKPIEPF; -.
DR OrthoDB; 795242at2759; -.
DR PhylomeDB; Q17766; -.
DR Reactome; R-CEL-196757; Metabolism of folate and pterines.
DR Reactome; R-CEL-196819; Vitamin B1 (thiamin) metabolism.
DR PRO; PR:Q17766; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00007388; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005542; F:folic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0090482; F:vitamin transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR002666; Folate_carrier.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10686; PTHR10686; 1.
DR Pfam; PF01770; Folate_carrier; 1.
DR PIRSF; PIRSF028739; Folate_carrier; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00806; rfc; 1.
PE 2: Evidence at transcript level;
KW Folate-binding; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..453
FT /note="Folate transporter 1"
FT /id="PRO_0000178664"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 260
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 273..274
FT /note="PD -> SE (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 286..287
FT /note="TA -> KS (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="L -> I (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="I -> M (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 314..315
FT /note="TS -> PY (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 324..325
FT /note="LC -> IW (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 330
FT /note="T -> S (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 332
FT /note="L -> M (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 334
FT /note="A -> G (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="S -> Y (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
FT CONFLICT 348..351
FT /note="LITA -> VRSV (in Ref. 3; CB397828)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 453 AA; 51077 MW; 22E3A3E2BBBD339D CRC64;
MSWRTTTAIL CLYGAVKEFR PATPFLTPFL ASPEKNITLD ELYSQVYPYW TYSYMLALIP
MFILTDILRY KPIVMIEAIG LVATWALLVF GKGVWQMQIM QVSFGVASAA EIAYYSYIYS
IVDRKHYKRA TSYIRAAALM GKLVAFGLGQ TLISTHTSDY LVLNQISLGA VCLVTIIAIF
LPRVKSEKAK VSMRAHEIVE QQTVESVESV QTPKAVKMSY TREYFKKISE ELQICSKNQE
LLKWSLWWAL ASCGVYQVQN YTQSLWKELQ NNPDDVANGV VEFVNTALGA FLSLFIHHLS
IDWTRHGQMI LFITSAIVAV LLYLCSQTTT VLVAYSSYVV ITSIYHMLIT AASANVAKEL
SSNNHGLIFG CNTFVAVCLQ SLLTLVVVDS RFLHLDIRTQ FVIYSGYFAL VASIFAFFFM
ISLFSKSSNA HTAQTTYEAT NEIQEETVFL DQN
