ID   FOLT_LACP3              Reviewed;         172 AA.
AC   Q035X6;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Folate transporter FolT;
DE   AltName: Full=Folate ECF transporter S component FolT;
GN   Name=folT; OrderedLocusNames=LSEI_2252;
OS   Lacticaseibacillus paracasei (strain ATCC 334 / BCRC 17002 / CCUG 31169 /
OS   CIP 107868 / KCTC 3260 / NRRL B-441) (Lactobacillus paracasei).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lacticaseibacillus.
OX   NCBI_TaxID=321967;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC   B-441;
RX   PubMed=17030793; DOI=10.1073/pnas.0607117103;
RA   Makarova K.S., Slesarev A., Wolf Y.I., Sorokin A., Mirkin B., Koonin E.V.,
RA   Pavlov A., Pavlova N., Karamychev V., Polouchine N., Shakhova V.,
RA   Grigoriev I., Lou Y., Rohksar D., Lucas S., Huang K., Goodstein D.M.,
RA   Hawkins T., Plengvidhya V., Welker D., Hughes J., Goh Y., Benson A.,
RA   Baldwin K., Lee J.-H., Diaz-Muniz I., Dosti B., Smeianov V., Wechter W.,
RA   Barabote R., Lorca G., Altermann E., Barrangou R., Ganesan B., Xie Y.,
RA   Rawsthorne H., Tamir D., Parker C., Breidt F., Broadbent J.R., Hutkins R.,
RA   O'Sullivan D., Steele J., Unlu G., Saier M.H. Jr., Klaenhammer T.,
RA   Richardson P., Kozyavkin S., Weimer B.C., Mills D.A.;
RT   "Comparative genomics of the lactic acid bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15611-15616(2006).
RN   [2]
RP   FOLATE-BINDING.
RC   STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC   B-441;
RX   PubMed=18776013; DOI=10.1128/jb.01070-08;
RA   Eudes A., Erkens G.B., Slotboom D.J., Rodionov D.A., Naponelli V.,
RA   Hanson A.D.;
RT   "Identification of genes encoding the folate- and thiamine-binding membrane
RT   proteins in Firmicutes.";
RL   J. Bacteriol. 190:7591-7594(2008).
RN   [3]
RP   FUNCTION AS A TRANSPORT COMPONENT, PROBABLE SUBUNIT, AND EXPRESSION IN
RP   L.LACTIS.
RC   STRAIN=ATCC 334 / BCRC 17002 / CCUG 31169 / CIP 107868 / KCTC 3260 / NRRL
RC   B-441;
RX   PubMed=18931129; DOI=10.1128/jb.01208-08;
RA   Rodionov D.A., Hebbeln P., Eudes A., ter Beek J., Rodionova I.A.,
RA   Erkens G.B., Slotboom D.J., Gelfand M.S., Osterman A.L., Hanson A.D.,
RA   Eitinger T.;
RT   "A novel class of modular transporters for vitamins in prokaryotes.";
RL   J. Bacteriol. 191:42-51(2009).
CC   -!- FUNCTION: Folate-binding protein that interacts with the energy-
CC       coupling factor (ECF) ABC-transporter complex. Unlike classic ABC
CC       transporters this ECF transporter provides the energy necessary to
CC       transport a number of different substrates. The substrates themselves
CC       are bound by transmembrane, not extracytoplasmic soluble proteins (By
CC       similarity). Binds folic acid with a dissociation constant of 9 nM;
CC       binds polyglutamyl folates less well. Upon coexpression with its
CC       energy-coupling factor (ECF) ABC-transporter complex EcfA1A2T in
CC       Lactococcus lactis subsp. cremoris (strain NZ9000) allows high level 5-
CC       formyltetrahydrofolate uptake; uptake requires both FolT and EcfA1A2T.
CC       Low levels of uptake occur in the presence of FolT alone, perhaps
CC       because it interacts with endogenous EcfA1A2T. {ECO:0000250,
CC       ECO:0000269|PubMed:18931129}.
CC   -!- SUBUNIT: In Lactococcus lactis subsp. cremoris (strain NZ9000) forms a
CC       stable energy-coupling factor (ECF) transporter complex composed of a
CC       membrane-embedded substrate-binding protein (S component), two ATP-
CC       binding proteins (A components) and a transmembrane protein (T
CC       component) (Probable). {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; CP000423; ABJ70996.1; -; Genomic_DNA.
DR   RefSeq; WP_003567081.1; NC_008526.1.
DR   RefSeq; YP_807438.1; NC_008526.1.
DR   AlphaFoldDB; Q035X6; -.
DR   SMR; Q035X6; -.
DR   STRING; 321967.LSEI_2252; -.
DR   EnsemblBacteria; ABJ70996; ABJ70996; LSEI_2252.
DR   GeneID; 61270376; -.
DR   KEGG; lca:LSEI_2252; -.
DR   PATRIC; fig|321967.11.peg.2215; -.
DR   HOGENOM; CLU_098232_3_0_9; -.
DR   OMA; WLHIMYG; -.
DR   Proteomes; UP000001651; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005542; F:folic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR030949; ECF_S_folate_fam.
DR   InterPro; IPR024529; ECF_trnsprt_substrate-spec.
DR   Pfam; PF12822; ECF_trnsprt; 1.
DR   TIGRFAMs; TIGR04518; ECF_S_folT_fam; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Folate-binding; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..172
FT                   /note="Folate transporter FolT"
FT                   /id="PRO_0000409016"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   172 AA;  19037 MW;  20416A73D0EBD76B CRC64;
     MQVLSFSSPK LSTRNMVYMA MLMAMQIVLG RFSFGTPWLK ISPAFFATIL MGYYFGPWLA
     AGAAALNDQL SIMIFSPGAN FPGFTISAAI AAMLYGMFFH GKKVTVLRTL LAVGMVLLIS
     NIILTTLWLN IMGTPWQGII WPRTIKNVVM LPIQTALSYG TLKAIERIRP HL