ID   FOXA4_XENTR             Reviewed;         399 AA.
AC   Q6P839;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Forkhead box protein A4;
DE            Short=FoxA4;
GN   Name=foxa4 {ECO:0000250|UniProtKB:P33205}; ORFNames=TGas072p11.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1] {ECO:0000312|EMBL:CAJ81986.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula {ECO:0000312|EMBL:CAJ81986.1};
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:CAJ81986.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula {ECO:0000312|EMBL:AAH61392.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcriptional repressor involved in embryonic nervous
CC       system development. Plays a role in the induction and patterning of the
CC       anterior-posterior neural axis. Involved in the establishment of floor
CC       plate differentiation from neural plate cells during gastrulation.
CC       Binds the anf1 promoter sequence to restrict expression of anf1 to the
CC       anterior of the neural plate, thereby patterning the forebrain. Can
CC       bind to the HNF-3-alpha DNA target sequence. Cooperates with t/bra in a
CC       dose-dependent manner to specify dorsal mesoderm formation, including
CC       notochord. May be involved in the dorso-ventral patterning of the
CC       mesoderm. Binds to DNA via the target sequence 5'-[GA]TAAA[TC]A-3',
CC       with 5'-GTAAATA-3' being the preferred binding site (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255, ECO:0000305}.
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DR   EMBL; CR762190; CAJ81986.1; -; mRNA.
DR   EMBL; BC061392; AAH61392.1; -; mRNA.
DR   RefSeq; NP_988938.1; NM_203607.1.
DR   AlphaFoldDB; Q6P839; -.
DR   SMR; Q6P839; -.
DR   STRING; 8364.ENSXETP00000055323; -.
DR   PaxDb; Q6P839; -.
DR   Ensembl; ENSXETT00000055323; ENSXETP00000055323; ENSXETG00000026171.
DR   GeneID; 394535; -.
DR   KEGG; xtr:394535; -.
DR   CTD; 394535; -.
DR   Xenbase; XB-GENE-486455; foxa4.
DR   eggNOG; KOG3563; Eukaryota.
DR   HOGENOM; CLU_027910_4_0_1; -.
DR   InParanoid; Q6P839; -.
DR   OMA; HNMASKN; -.
DR   OrthoDB; 1181467at2759; -.
DR   PhylomeDB; Q6P839; -.
DR   Proteomes; UP000008143; Chromosome 4.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000026171; Expressed in gastrula and 7 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0019904; F:protein domain specific binding; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00059; FH; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR013638; Fork-head_N.
DR   InterPro; IPR001766; Fork_head_dom.
DR   InterPro; IPR018533; Forkhead_box_C.
DR   InterPro; IPR018122; TF_fork_head_CS_1.
DR   InterPro; IPR030456; TF_fork_head_CS_2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00250; Forkhead; 1.
DR   Pfam; PF08430; Forkhead_N; 1.
DR   Pfam; PF09354; HNF_C; 1.
DR   PRINTS; PR00053; FORKHEAD.
DR   SMART; SM00339; FH; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00657; FORK_HEAD_1; 1.
DR   PROSITE; PS00658; FORK_HEAD_2; 1.
DR   PROSITE; PS50039; FORK_HEAD_3; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..399
FT                   /note="Forkhead box protein A4"
FT                   /id="PRO_0000250437"
FT   DNA_BIND        119..213
FT                   /note="Fork-head"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT   REGION          219..290
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        219..235
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        246..290
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   399 AA;  44437 MW;  96FFD224A6172B23 CRC64;
     MLNRVKLEIK DPMDWNTMYQ ESEIYSGIHN MNNGLPSNSF LPTDVSTVPT SMPYMSNGLS
     GPVTSIQGNL GSLGSMTQGM VGSLAPPAST PAYPMGYCQG ESEFQRDPRT YRRNYSHAKP
     PYSYISLITM AIQQAPNKMM TLNEIYQWII DLFPYYRQNQ QRWQNSIRHS LSFNDCFVKV
     PRSPEKPGKG SYWTLHPESG NMFENGCYLR RQKRFKCERS KSGEREKKVN KPGDENGGSL
     KETPVGYDDC SSSRSPQAPV NDGGRDSTGS SIHQASGGSP VGLSPTSEQA GTASQLMYPL
     GLSNDGYLGL VGEDVHLKHD PFSGRHPFSI TQLMSSEQDQ TYPSKLEMCP TTDHLVHYSN
     YSSDYHNMAS KNGLDMQTSS STDNGYYANM YSRPILSSL