FOXD3_MOUSE
ID FOXD3_MOUSE Reviewed; 465 AA.
AC Q61060;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 3.
DT 25-MAY-2022, entry version 155.
DE RecName: Full=Forkhead box protein D3;
DE AltName: Full=HNF3/FH transcription factor genesis;
DE AltName: Full=Hepatocyte nuclear factor 3 forkhead homolog 2;
DE Short=HFH-2;
GN Name=Foxd3; Synonyms=Hfh2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC TISSUE=Embryonic carcinoma;
RX PubMed=8798505; DOI=10.1074/jbc.271.38.23126;
RA Sutton J., Costa R., Klug M., Field L., Xu D., Largaespada D.A.,
RA Fletcher C.F., Jenkins N.A., Copeland N.G., Klemsz M., Hromas R.;
RT "Genesis, a winged helix transcriptional repressor with expression
RT restricted to embryonic stem cells.";
RL J. Biol. Chem. 271:23126-23133(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=9767163; DOI=10.1016/s0925-4773(98)00105-1;
RA Labosky P.A., Kaestner K.H.;
RT "The winged helix transcription factor Hfh2 is expressed in neural crest
RT and spinal cord during mouse development.";
RL Mech. Dev. 76:185-190(1998).
RN [3]
RP SEQUENCE REVISION.
RA Kessler D.S., Labosky P.A., Kaestner K.H.;
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12381664; DOI=10.1101/gad.1020502;
RA Hanna L.A., Foreman R.K., Tarasenko I.A., Kessler D.S., Labosky P.A.;
RT "Requirement for Foxd3 in maintaining pluripotent cells of the early mouse
RT embryo.";
RL Genes Dev. 16:2650-2661(2002).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=11684651; DOI=10.1242/dev.128.21.4127;
RA Dottori M., Gross M.K., Labosky P., Goulding M.;
RT "The winged-helix transcription factor Foxd3 suppresses interneuron
RT differentiation and promotes neural crest cell fate.";
RL Development 128:4127-4138(2001).
CC -!- FUNCTION: Binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and
CC acts as a transcriptional repressor. Also acts as a transcriptional
CC activator. Promotes development of neural crest cells from neural tube
CC progenitors. Restricts neural progenitor cells to the neural crest
CC lineage while suppressing interneuron differentiation. Required for
CC maintenance of pluripotent cells in the pre-implantation and peri-
CC implantation stages of embryogenesis. {ECO:0000269|PubMed:12381664,
CC ECO:0000269|PubMed:8798505}.
CC -!- SUBUNIT: Interacts with POU5F1. {ECO:0000250|UniProtKB:Q9UJU5}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in premigratory and migrating neural
CC crest cells in the early embryo and in motorneuron and interneuron
CC progenitors in the developing spinal cord.
CC {ECO:0000269|PubMed:11684651, ECO:0000269|PubMed:9767163}.
CC -!- DEVELOPMENTAL STAGE: During early embryogenesis, not expressed in
CC unfertilized oocytes or fertilized one-cell embryos but detected in
CC blastocysts. {ECO:0000269|PubMed:12381664}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA87569.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U41047; AAA87569.1; ALT_FRAME; mRNA.
DR EMBL; AF067421; AAC28352.2; -; mRNA.
DR EMBL; BX005053; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_034555.3; NM_010425.3.
DR AlphaFoldDB; Q61060; -.
DR SMR; Q61060; -.
DR STRING; 10090.ENSMUSP00000084541; -.
DR PhosphoSitePlus; Q61060; -.
DR PaxDb; Q61060; -.
DR PRIDE; Q61060; -.
DR ProteomicsDB; 271592; -.
DR DNASU; 15221; -.
DR GeneID; 15221; -.
DR KEGG; mmu:15221; -.
DR CTD; 27022; -.
DR MGI; MGI:1347473; Foxd3.
DR eggNOG; KOG2294; Eukaryota.
DR InParanoid; Q61060; -.
DR OrthoDB; 1270467at2759; -.
DR BioGRID-ORCS; 15221; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Foxd3; mouse.
DR PRO; PR:Q61060; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q61060; protein.
DR GO; GO:0000785; C:chromatin; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; TAS:MGI.
DR GO; GO:0003677; F:DNA binding; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003690; F:double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:MGI.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEP:MGI.
DR GO; GO:0001892; P:embryonic placenta development; IMP:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IDA:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; TAS:MGI.
DR GO; GO:0001829; P:trophectodermal cell differentiation; IMP:MGI.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..465
FT /note="Forkhead box protein D3"
FT /id="PRO_0000091818"
FT DNA_BIND 130..221
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 1..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 375..405
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..39
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 465 AA; 46345 MW; 7277EB2EE2C8E0C2 CRC64;
MTLSGSGSAS DMSGQTVLTA EDVDIDVVGE GDDGLEEKDS DAGCDSPAGP PDLRLDEADE
GPPVSAHHGQ SQPQALALPT EATGPGNDTG APEADGCKGG EDAVTGGGGP GAGSGATGGL
TPNKPKNSLV KPPYSYIALI TMAILQSPQK KLTLSGICEF ISNRFPYYRE KFPAWQNSIR
HNLSLNDCFV KIPREPGNPG KGNYWTLDPQ SEDMFDNGSF LRRRKRFKRH QQEHLREQTA
LMMQSFGAYS LAAAAGAGPY GLHPAAAAGA YSHPAAAAAA AAAAALQYPY ALPPVAPVLP
PAVPLLPSGE LGRKAAAFGS QLGPSLQLQL NTLGAAAAAA GTAGAAGTTS LIKSEPSARP
SFSIENIIGA GSAAPGGSAG GGGSGGGAGG GGGSGGGGGA QSFLRPPGTV QSAALMATHQ
PLSLSRTTAT IAPILSVPLS GQFLQPAASA AAAAAAAVQA KWPAQ
