FOXH1_DANRE
ID FOXH1_DANRE Reviewed; 472 AA.
AC Q9I9E1; Q9I8K6;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Forkhead box protein H1;
DE AltName: Full=Forkhead activin signal transducer 1;
DE Short=Fast-1;
DE AltName: Full=Schmalspur protein;
GN Name=foxh1; Synonyms=fast1, sur;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, MUTAGENESIS OF ARG-94 AND LYS-97, AND DISRUPTION PHENOTYPE.
RX PubMed=10996071; DOI=10.1016/s0960-9822(00)00669-2;
RA Pogoda H.-M., Solnica-Krezel L., Driever W., Meyer D.;
RT "The zebrafish forkhead transcription factor FoxH1/Fast1 is a modulator of
RT nodal signaling required for organizer formation.";
RL Curr. Biol. 10:1041-1049(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS
RP OF ARG-94.
RC TISSUE=Embryo;
RX PubMed=10996072; DOI=10.1016/s0960-9822(00)00679-5;
RA Sirotkin H.I., Gates M.A., Kelly P.D., Schier A.F., Talbot W.S.;
RT "Fast1 is required for the development of dorsal axial structures in
RT zebrafish.";
RL Curr. Biol. 10:1051-1054(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=11091091; DOI=10.1016/s0925-4773(00)00485-8;
RA Boggetti B., Argenton F., Haffter P., Bianchi M.E., Cotelli F.,
RA Beltrame M.;
RT "Cloning and expression pattern of a zebrafish homolog of forkhead activin
RT signal transducer (FAST), a transcription factor mediating Nodal-related
RT signals.";
RL Mech. Dev. 99:187-190(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcriptional activator. Activates an activin response
CC element (ARE). Recognizes and binds to the DNA sequence 5'-
CC TGT[GT][GT]ATT-3'. Modulator of nodal signaling required for organizer
CC formation. Also required for the development of dorsal axial structures
CC and left-right symmetry. {ECO:0000269|PubMed:10996071,
CC ECO:0000269|PubMed:10996072}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089,
CC ECO:0000269|PubMed:10996071}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Localized to the prospective animal pole during oogenesis. Ubiquitous
CC expression during the blastula stages. During early gastrulation,
CC levels are higher ventrally and in the presumptive shield (organizer
CC region). Later in gastrulation, expression is restricted to midline and
CC ventral cells. At the start of somatogenesis, expression is restricted
CC to notochord, lateral plate mesoderm and a stripe of anterior dorsal
CC neuroectoderm. By the 19-somite stage, expression in the lateral plate
CC and midline diminishes but forebrain expression persists. All
CC expression is lost by 24 hours post-fertilization (hpf).
CC {ECO:0000269|PubMed:10996071, ECO:0000269|PubMed:10996072,
CC ECO:0000269|PubMed:11091091}.
CC -!- DOMAIN: The FM region is required for binding smad2/smad4 complexes.
CC FM2 is more effective than FM1 and only interacts with phosphorylated
CC smad2 that is in an activated smad complex (By similarity).
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Fishes exhibit ventral body curvature, reduced
CC spacing of the eyes, and absence or reduction of ventral neuroectoderm,
CC including the floor plate. {ECO:0000269|PubMed:10996071}.
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DR EMBL; AF263000; AAF72712.1; -; mRNA.
DR EMBL; AF264751; AAF81247.1; -; mRNA.
DR EMBL; BC044340; AAH44340.1; -; mRNA.
DR RefSeq; NP_571577.1; NM_131502.1.
DR AlphaFoldDB; Q9I9E1; -.
DR SMR; Q9I9E1; -.
DR STRING; 7955.ENSDARP00000127073; -.
DR PaxDb; Q9I9E1; -.
DR GeneID; 57930; -.
DR KEGG; dre:57930; -.
DR CTD; 8928; -.
DR ZFIN; ZDB-GENE-000616-15; foxh1.
DR eggNOG; KOG2294; Eukaryota.
DR InParanoid; Q9I9E1; -.
DR OrthoDB; 1270467at2759; -.
DR PhylomeDB; Q9I9E1; -.
DR PRO; PR:Q9I9E1; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0000785; C:chromatin; IDA:ZFIN.
DR GO; GO:0005634; C:nucleus; IC:ZFIN.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:ZFIN.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:ZFIN.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:ZFIN.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:ZFIN.
DR GO; GO:0046332; F:SMAD binding; IMP:ZFIN.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:ZFIN.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0048319; P:axial mesoderm morphogenesis; IMP:ZFIN.
DR GO; GO:0048327; P:axial mesodermal cell fate specification; IMP:ZFIN.
DR GO; GO:0001568; P:blood vessel development; IMP:ZFIN.
DR GO; GO:0048854; P:brain morphogenesis; IMP:ZFIN.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0042074; P:cell migration involved in gastrulation; IMP:ZFIN.
DR GO; GO:0071345; P:cellular response to cytokine stimulus; ISS:UniProtKB.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR GO; GO:0009953; P:dorsal/ventral pattern formation; IMP:ZFIN.
DR GO; GO:0060971; P:embryonic heart tube left/right pattern formation; IMP:ZFIN.
DR GO; GO:0003143; P:embryonic heart tube morphogenesis; IMP:ZFIN.
DR GO; GO:0001706; P:endoderm formation; IMP:ZFIN.
DR GO; GO:0033504; P:floor plate development; IMP:ZFIN.
DR GO; GO:0021508; P:floor plate formation; IMP:ZFIN.
DR GO; GO:0001947; P:heart looping; IMP:ZFIN.
DR GO; GO:0021854; P:hypothalamus development; IMP:ZFIN.
DR GO; GO:0046619; P:lens placode formation involved in camera-type eye formation; IMP:ZFIN.
DR GO; GO:0048382; P:mesendoderm development; IGI:ZFIN.
DR GO; GO:0001707; P:mesoderm formation; IMP:ZFIN.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:ZFIN.
DR GO; GO:0030903; P:notochord development; IMP:ZFIN.
DR GO; GO:0014028; P:notochord formation; IMP:ZFIN.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0021501; P:prechordal plate formation; IMP:ZFIN.
DR GO; GO:2000223; P:regulation of BMP signaling pathway involved in heart jogging; IMP:ZFIN.
DR GO; GO:0010468; P:regulation of gene expression; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IGI:ZFIN.
DR GO; GO:0032525; P:somite rostral/caudal axis specification; IMP:ZFIN.
DR GO; GO:0010159; P:specification of animal organ position; IMP:ZFIN.
DR GO; GO:0060063; P:Spemann organizer formation at the embryonic shield; IMP:ZFIN.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..472
FT /note="Forkhead box protein H1"
FT /id="PRO_0000091844"
FT DNA_BIND 97..193
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 36..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 339..465
FT /note="SMAD-interaction domain (SID)"
FT MOTIF 357..361
FT /note="Fast/FoxH1 motif 1 (FM1)"
FT MOTIF 367..373
FT /note="Fast/FoxH1 motif 2 (FM2)"
FT MOTIF 428..448
FT /note="SMAD interaction motif (SIM)"
FT COMPBIAS 220..242
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 337..354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 94
FT /note="R->H: In sur(m768); loss of function."
FT /evidence="ECO:0000269|PubMed:10996071,
FT ECO:0000269|PubMed:10996072"
FT MUTAGEN 97
FT /note="K->N: In sur(ty68b); loss of function."
FT /evidence="ECO:0000269|PubMed:10996071"
FT CONFLICT 233
FT /note="Q -> H (in Ref. 3; AAF81247)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="G -> R (in Ref. 3; AAF81247)"
FT /evidence="ECO:0000305"
FT CONFLICT 395
FT /note="G -> S (in Ref. 3; AAF81247)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 472 AA; 51620 MW; EC6285F1BFA970CA CRC64;
MTKHWGGPGL LAPPVITVGE GAQRDHHLDC RIGYSSSKRS CHRSSNPLLE LGGRLDKSTG
MAQDSCYRAK ATNQGPWELQ DGNSSGGKKK NYQRYPKPPY SYLAMIAMVI QNSPEKKLTL
SEILKEISTL FPFFKGNYKG WRDSVRHNLS SYDCFVKVLK DPGKPQGKGN FWTVEVNRIP
LELLKRQNTA VSRQDETIFA QDLAPYIFQG YSQPNKSKPL PPESSLPPVP TRQSPPPSED
PYRPKLDSTF AIDSLLHSLR PASSAGEGLR ERESWGVGPP PHTRSTTPPR PCNASYNGSS
SASSVSPASD FSDEDWRGVT VVGKRSGDRG ITSDAYSDSC PPPNKSSKRG NTPPWELPTS
YAKYTPPNAV APPSMRFNGN PFMPLGGIPF YGYGGAHVTT SHLIGHPYWP ILPSGPVSIQ
APPLLMDLDS MLQSVPPNKS VFDALGSNNQ TVHPSPNQYA LQNGPSLCKY SL
