FOXJ1_MOUSE
ID FOXJ1_MOUSE Reviewed; 421 AA.
AC Q61660; Q3US42;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Forkhead box protein J1 {ECO:0000305};
DE AltName: Full=Hepatocyte nuclear factor 3 forkhead homolog 4 {ECO:0000303|PubMed:7683413};
DE Short=HFH-4 {ECO:0000303|PubMed:7683413};
GN Name=Foxj1 {ECO:0000312|MGI:MGI:1347474};
GN Synonyms=Hfh4 {ECO:0000303|PubMed:7683413};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6 X CBA; TISSUE=Lung;
RX PubMed=7683413; DOI=10.1073/pnas.90.9.3948;
RA Clevidence D.E., Overdier D.G., Tao W., Qian X., Pani L., Lai E.,
RA Costa R.H.;
RT "Identification of nine tissue-specific transcription factors of the
RT hepatocyte nuclear factor 3/forkhead DNA-binding-domain family.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:3948-3952(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=9096351; DOI=10.1073/pnas.94.7.3094;
RA Lim L., Zhou H., Costa R.H.;
RT "The winged helix transcription factor HFH-4 is expressed during choroid
RT plexus epithelial development in the mouse embryo.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:3094-3099(1997).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9739041; DOI=10.1172/jci4786;
RA Chen J., Knowles H.J., Hebert J.L., Hackett B.P.;
RT "Mutation of the mouse hepatocyte nuclear factor/forkhead homologue 4 gene
RT results in an absence of cilia and random left-right asymmetry.";
RL J. Clin. Invest. 102:1077-1082(1998).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10873152; DOI=10.1165/ajrcmb.23.1.4070;
RA Brody S.L., Yan X.H., Wuerffel M.K., Song S.K., Shapiro S.D.;
RT "Ciliogenesis and left-right axis defects in forkhead factor HFH-4-null
RT mice.";
RL Am. J. Respir. Cell Mol. Biol. 23:45-51(2000).
RN [8]
RP FUNCTION.
RX PubMed=14996907; DOI=10.1242/jcs.00978;
RA Gomperts B.N., Gong-Cooper X., Hackett B.P.;
RT "Foxj1 regulates basal body anchoring to the cytoskeleton of ciliated
RT pulmonary epithelial cells.";
RL J. Cell Sci. 117:1329-1337(2004).
RN [9]
RP FUNCTION.
RX PubMed=22357932; DOI=10.1242/dev.072728;
RA Alten L., Schuster-Gossler K., Beckers A., Groos S., Ulmer B.,
RA Hegermann J., Ochs M., Gossler A.;
RT "Differential regulation of node formation, nodal ciliogenesis and cilia
RT positioning by Noto and Foxj1.";
RL Development 139:1276-1284(2012).
RN [10]
RP FUNCTION.
RX PubMed=27965440; DOI=10.1242/dev.139626;
RA Weidemann M., Schuster-Gossler K., Stauber M., Wrede C., Hegermann J.,
RA Ott T., Boldt K., Beyer T., Serth K., Kremmer E., Blum M., Ueffing M.,
RA Gossler A.;
RT "CFAP157 is a murine downstream effector of FOXJ1 that is specifically
RT required for flagellum morphogenesis and sperm motility.";
RL Development 143:4736-4748(2016).
RN [11]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=27914912; DOI=10.1016/j.ydbio.2016.11.019;
RA Stauber M., Weidemann M., Dittrich-Breiholz O., Lobschat K., Alten L.,
RA Mai M., Beckers A., Kracht M., Gossler A.;
RT "Identification of FOXJ1 effectors during ciliogenesis in the foetal
RT respiratory epithelium and embryonic left-right organiser of the mouse.";
RL Dev. Biol. 423:170-188(2017).
RN [12]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=28666954; DOI=10.1016/j.ydbio.2017.06.027;
RA Stauber M., Boldt K., Wrede C., Weidemann M., Kellner M.,
RA Schuster-Gossler K., Kuehnel M.P., Hegermann J., Ueffing M., Gossler A.;
RT "1700012B09Rik, a FOXJ1 effector gene active in ciliated tissues of the
RT mouse but not essential for motile ciliogenesis.";
RL Dev. Biol. 429:186-199(2017).
CC -!- FUNCTION: Transcription factor specifically required for the formation
CC of motile cilia (PubMed:9096351, PubMed:9739041, PubMed:10873152,
CC PubMed:14996907, PubMed:22357932, PubMed:27965440). Acts by activating
CC transcription of genes that mediate assembly of motile cilia, such as
CC CFAP157 (PubMed:27965440). Binds the DNA consensus sequences 5'-
CC HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T,
CC D is not C, and K is G or T) (PubMed:9096351). Activates the
CC transcription of a variety of ciliary proteins in the developing brain
CC and lung (PubMed:28666954, PubMed:27914912).
CC {ECO:0000269|PubMed:10873152, ECO:0000269|PubMed:14996907,
CC ECO:0000269|PubMed:22357932, ECO:0000269|PubMed:27914912,
CC ECO:0000269|PubMed:27965440, ECO:0000269|PubMed:28666954,
CC ECO:0000269|PubMed:9096351, ECO:0000269|PubMed:9739041}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:9096351}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in tissues containing
CC motile cilia. {ECO:0000269|PubMed:27914912,
CC ECO:0000269|PubMed:28666954}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing fetal lung epithelium
CC and developing brain (at protein level). {ECO:0000269|PubMed:27914912,
CC ECO:0000269|PubMed:28666954}.
CC -!- DISRUPTION PHENOTYPE: Mice lack motile respiratory tract cilia and
CC exhibit randomization of the left-right body axis due to loss of motile
CC cilia in the embryonic node (PubMed:9739041, PubMed:10873152). Motile
CC type cilia with a '9 + 2' microtubule ultrastructure are absent in
CC epithelial cells, including those in the airways (PubMed:10873152).
CC {ECO:0000269|PubMed:10873152, ECO:0000269|PubMed:9739041}.
CC -!- SIMILARITY: Belongs to the FOXJ1 family. {ECO:0000305}.
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DR EMBL; L13204; AAA21689.1; -; mRNA.
DR EMBL; AK140847; BAE24495.1; -; mRNA.
DR EMBL; AL645861; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466558; EDL34572.1; -; Genomic_DNA.
DR CCDS; CCDS25665.1; -.
DR PIR; I49734; I49734.
DR RefSeq; NP_032266.3; NM_008240.3.
DR RefSeq; XP_006532332.1; XM_006532269.2.
DR AlphaFoldDB; Q61660; -.
DR SMR; Q61660; -.
DR BioGRID; 200289; 1.
DR IntAct; Q61660; 1.
DR STRING; 10090.ENSMUSP00000038351; -.
DR iPTMnet; Q61660; -.
DR PhosphoSitePlus; Q61660; -.
DR MaxQB; Q61660; -.
DR PaxDb; Q61660; -.
DR PRIDE; Q61660; -.
DR ProteomicsDB; 267497; -.
DR Antibodypedia; 1443; 250 antibodies from 32 providers.
DR DNASU; 15223; -.
DR Ensembl; ENSMUST00000036215; ENSMUSP00000038351; ENSMUSG00000034227.
DR GeneID; 15223; -.
DR KEGG; mmu:15223; -.
DR UCSC; uc007mkv.2; mouse.
DR CTD; 2302; -.
DR MGI; MGI:1347474; Foxj1.
DR VEuPathDB; HostDB:ENSMUSG00000034227; -.
DR eggNOG; KOG2294; Eukaryota.
DR GeneTree; ENSGT00940000156895; -.
DR HOGENOM; CLU_050055_0_0_1; -.
DR InParanoid; Q61660; -.
DR OMA; FKKRRMP; -.
DR OrthoDB; 1270467at2759; -.
DR PhylomeDB; Q61660; -.
DR TreeFam; TF333250; -.
DR BioGRID-ORCS; 15223; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Foxj1; mouse.
DR PRO; PR:Q61660; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q61660; protein.
DR Bgee; ENSMUSG00000034227; Expressed in choroid plexus of fourth ventricle and 102 other tissues.
DR Genevisible; Q61660; MM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISO:MGI.
DR GO; GO:0030036; P:actin cytoskeleton organization; IMP:BHF-UCL.
DR GO; GO:0090630; P:activation of GTPase activity; IDA:BHF-UCL.
DR GO; GO:0035082; P:axoneme assembly; ISS:UniProtKB.
DR GO; GO:0007420; P:brain development; IEP:BHF-UCL.
DR GO; GO:0002508; P:central tolerance induction; IMP:BHF-UCL.
DR GO; GO:0032053; P:ciliary basal body organization; ISS:UniProtKB.
DR GO; GO:0060271; P:cilium assembly; IMP:UniProtKB.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0060429; P:epithelium development; IEP:BHF-UCL.
DR GO; GO:0035089; P:establishment of apical/basal cell polarity; IMP:MGI.
DR GO; GO:0072016; P:glomerular parietal epithelial cell development; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0006959; P:humoral immune response; IMP:BHF-UCL.
DR GO; GO:0060972; P:left/right pattern formation; IMP:MGI.
DR GO; GO:0050900; P:leukocyte migration; IMP:BHF-UCL.
DR GO; GO:0060428; P:lung epithelium development; IEA:Ensembl.
DR GO; GO:0035502; P:metanephric part of ureteric bud development; IEA:Ensembl.
DR GO; GO:0044458; P:motile cilium assembly; IMP:UniProtKB.
DR GO; GO:0050869; P:negative regulation of B cell activation; IMP:BHF-UCL.
DR GO; GO:0002635; P:negative regulation of germinal center formation; IMP:BHF-UCL.
DR GO; GO:0002924; P:negative regulation of humoral immune response mediated by circulating immunoglobulin; IMP:BHF-UCL.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; IMP:BHF-UCL.
DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IMP:BHF-UCL.
DR GO; GO:0033085; P:negative regulation of T cell differentiation in thymus; IMP:BHF-UCL.
DR GO; GO:0042130; P:negative regulation of T cell proliferation; IMP:BHF-UCL.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:BHF-UCL.
DR GO; GO:0002897; P:positive regulation of central B cell tolerance induction; IMP:BHF-UCL.
DR GO; GO:1901248; P:positive regulation of lung ciliated cell differentiation; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0008104; P:protein localization; ISS:UniProtKB.
DR GO; GO:0030856; P:regulation of epithelial cell differentiation; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00059; FH; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR001766; Fork_head_dom.
DR InterPro; IPR018122; TF_fork_head_CS_1.
DR InterPro; IPR030456; TF_fork_head_CS_2.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00250; Forkhead; 1.
DR PRINTS; PR00053; FORKHEAD.
DR SMART; SM00339; FH; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00657; FORK_HEAD_1; 1.
DR PROSITE; PS00658; FORK_HEAD_2; 1.
DR PROSITE; PS50039; FORK_HEAD_3; 1.
PE 1: Evidence at protein level;
KW Activator; Cilium biogenesis/degradation; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..421
FT /note="Forkhead box protein J1"
FT /id="PRO_0000091851"
FT DNA_BIND 120..210
FT /note="Fork-head"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 275..276
FT /note="KQ -> NE (in Ref. 1; AAA21689)"
FT /evidence="ECO:0000305"
FT CONFLICT 317
FT /note="A -> R (in Ref. 1; AAA21689)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 421 AA; 45474 MW; B5EB01D747672DFA CRC64;
MAESWLRLCG AGPGEEAGPE GGMEEPDALD DSLTSLQWLQ EFSILNAKAP TLPPGGTDPH
GYHQVPGLVA PGSPLAADPA CLGQPHTPGK PTSSCTSRSA PPGLQAPPPD DVDYATNPHV
KPPYSYATLI CMAMQASKAT KITLSAIYKW ITDNFCYFRH ADPTWQNSIR HNLSLNKCFI
KVPREKDEPG KGGFWRIDPQ YAERLLSGAF KKRRLPPVHI HPAFARQASQ EPSAAPWGGP
LTVNREAQQL LQEFEEATGE GGWGTGEGRL GHKRKQPLPK RVAKVLRPPS TLLLTQEEQG
ELEPLKGNFD WEAIFEAGAL GEELSSLEGL ELSPPLSPSS HGDVDLTVHG RHINCPATWG
PPAEQAADSL DFDETFLATS FLQHPWDESG SGCLPPEPIF EAGDATLAAD LQDWASVGAF
L
