ID   FOXN4_XENLA             Reviewed;         506 AA.
AC   Q3BJS1;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Forkhead box protein N4;
GN   Name=foxn4 {ECO:0000312|EMBL:CAJ38821.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:CAJ38821.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Embryo {ECO:0000269|PubMed:16525939};
RX   PubMed=16525939; DOI=10.1387/ijdb.052126ms;
RA   Schuff M., Roessner A., Donow C., Knoechel W.;
RT   "Temporal and spatial expression patterns of FoxN genes in Xenopus laevis
RT   embryos.";
RL   Int. J. Dev. Biol. 50:429-434(2006).
CC   -!- FUNCTION: Transcription factor that may play a role in neural and some
CC       non-neural tissues development, such as retina and lung respectively.
CC       Binds to an 11-bp consensus sequence containing the invariant
CC       tetranucleotide 5'-ACGC-3' (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Predominantly localized to the developing eye.
CC       Expressed in the eye anlage at stage 18, where expression increases
CC       between stages 21 and 26. At stage 26, localizes to the prospective
CC       retina. At stage 29/30, expression is strong within the eye vesicle, in
CC       the midbrain and in the nephrostomes of the pronephric kidney. After
CC       stage 30, expression in the nephrostomes decreases until stage 33/34
CC       where it is largely absent. Expression in the eye decreases after stage
CC       33/34 until stage 38 where only a few cells in the ciliary marginal
CC       zone (CMZ) show expression. {ECO:0000269|PubMed:16525939}.
CC   -!- DEVELOPMENTAL STAGE: Expressed zygotically. Expression begins after
CC       mid-blastula transition, diminishes during gastrulation and increases
CC       again at stage 15, remaining until stage 45.
CC       {ECO:0000269|PubMed:16525939}.
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DR   EMBL; AM114796; CAJ38821.1; -; mRNA.
DR   RefSeq; NP_001089121.1; NM_001095652.1.
DR   AlphaFoldDB; Q3BJS1; -.
DR   SMR; Q3BJS1; -.
DR   GeneID; 733405; -.
DR   KEGG; xla:733405; -.
DR   CTD; 733405; -.
DR   Xenbase; XB-GENE-981090; foxn4.S.
DR   OrthoDB; 438804at2759; -.
DR   Proteomes; UP000186698; Chromosome 1S.
DR   Bgee; 733405; Expressed in testis and 6 other tissues.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR   GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR   GO; GO:0043565; F:sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0035881; P:amacrine cell differentiation; ISS:UniProtKB.
DR   GO; GO:0036302; P:atrioventricular canal development; ISS:UniProtKB.
DR   GO; GO:0001947; P:heart looping; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   GO; GO:0008016; P:regulation of heart contraction; ISS:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0010842; P:retina layer formation; ISS:UniProtKB.
DR   GO; GO:0060579; P:ventral spinal cord interneuron fate commitment; ISS:UniProtKB.
DR   CDD; cd00059; FH; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR001766; Fork_head_dom.
DR   InterPro; IPR030456; TF_fork_head_CS_2.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00250; Forkhead; 1.
DR   PRINTS; PR00053; FORKHEAD.
DR   SMART; SM00339; FH; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00658; FORK_HEAD_2; 1.
DR   PROSITE; PS50039; FORK_HEAD_3; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Neurogenesis; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..506
FT                   /note="Forkhead box protein N4"
FT                   /id="PRO_0000247736"
FT   DNA_BIND        190..286
FT                   /note="Fork-head"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00089"
SQ   SEQUENCE   506 AA;  55919 MW;  93297300175BFB8C CRC64;
     MIDSDISAIM SGIIRTSEQN HHPQSQEYRH MNPSQMGAEE LPNDLQSLSW LTSVDVPRLQ
     QMTNTRMDYS HTSQNPLMQQ SGHLSNNVHS PGSMIHVPTS MQQGILGLST LTSHGSNMSQ
     YSMAGHVSPS LQAQQPVYQT HSQQIYGLSQ GSQCSSANLY NSSYRAQAQF TQPHLAAHPT
     QELQPKSYPK PIYSYSCLIA MALKNSKTGS LPVSEIYSFM KDHFLYFKTA PDGWKNSVRH
     NLSLNKCFEK VENRLSGSSR KGCLWALNPA KIDKMEEEMQ KWKRKDLPAI RRSMANPDEL
     DKLIMDRPEN CKAPSKLVES EVQAMTTIGN LPGRIPQHQP HRVMTLSLQS IPLHHQIQTQ
     ARIASGSPAP AQSPPIHSVP DMTHSPLQQH IMSRAASDFL SLTSDMNTEV DALDPSIMDF
     ALQGNIWEAM KDESFSLDTL GAFSSSPLQL SDCDLGTIGL TPVSSSGDHS FSDFQVTSLY
     TTYPTLENVA PSQCVPGSGT KPIALL