FPA_ARATH
ID FPA_ARATH Reviewed; 901 AA.
AC Q8LPQ9; A8MQX1; B9DG59; C0Z2L9; O22855; Q58T22;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-MAR-2010, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Flowering time control protein FPA;
GN Name=FPA; OrderedLocusNames=At2g43410; ORFNames=T1O24.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=11402170; DOI=10.2307/3871305;
RA Schomburg F.M., Patton D.A., Meinke D.W., Amasino R.M.;
RT "FPA, a gene involved in floral induction in Arabidopsis, encodes a protein
RT containing RNA-recognition motifs.";
RL Plant Cell 13:1427-1436(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Lz-0;
RX PubMed=15911588; DOI=10.1534/genetics.104.036533;
RA Werner J.D., Borevitz J.O., Uhlenhaut N.H., Ecker J.R., Chory J.,
RA Weigel D.;
RT "FRIGIDA-independent variation in flowering time of natural Arabidopsis
RT thaliana accessions.";
RL Genetics 170:1197-1207(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17916737; DOI=10.1126/science.1146565;
RA Baurle I., Smith L., Baulcombe D.C., Dean C.;
RT "Widespread role for the flowering-time regulators FCA and FPA in RNA-
RT mediated chromatin silencing.";
RL Science 318:109-112(2007).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=20079695; DOI=10.1016/j.devcel.2009.12.009;
RA Hornyik C., Terzi L.C., Simpson G.G.;
RT "The spen family protein FPA controls alternative cleavage and
RT polyadenylation of RNA.";
RL Dev. Cell 18:203-213(2010).
CC -!- FUNCTION: Plays a role in the regulation of flowering time in the
CC autonomous flowering pathway by decreasing FLOWERING LOCUS C mRNA
CC levels. Required for RNA-mediated chromatin silencing of a range of
CC loci in the genome. Cotranscriptionally recognizes aberrant RNA and
CC marks it for silencing. Controls alternative cleavage and
CC polyadenylation on pre-mRNAs and antisense RNAs. Acts redundantly with
CC FCA to prevent the expression of distally polyadenylated antisense RNAs
CC at the FLC locus. {ECO:0000269|PubMed:11402170,
CC ECO:0000269|PubMed:17916737, ECO:0000269|PubMed:20079695}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17916737}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8LPQ9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LPQ9-2; Sequence=VSP_038751;
CC Name=3;
CC IsoId=Q8LPQ9-3; Sequence=VSP_038750, VSP_038752;
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers.
CC Highest expression in flower stems and meristematic regions.
CC {ECO:0000269|PubMed:11402170}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout the plant life cycle.
CC {ECO:0000269|PubMed:11402170}.
CC -!- INDUCTION: Negative feedback mediated by FPA itself.
CC {ECO:0000269|PubMed:20079695}.
CC -!- DISRUPTION PHENOTYPE: Late flowering. {ECO:0000269|PubMed:11402170}.
CC -!- MISCELLANEOUS: While FCA requires both FY and FLD, FPA requires FLD but
CC not FY to repress FLC.
CC -!- SIMILARITY: Belongs to the RRM Spen family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB64314.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAX51263.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY849993; AAX51263.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC002335; AAB64314.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10262.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10263.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10264.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10265.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61362.1; -; Genomic_DNA.
DR EMBL; AY094463; AAM19833.1; -; mRNA.
DR EMBL; BT003029; AAO23594.1; -; mRNA.
DR EMBL; AK317032; BAH19726.1; -; mRNA.
DR EMBL; AK318833; BAH56948.1; -; mRNA.
DR RefSeq; NP_001078046.1; NM_001084577.2. [Q8LPQ9-1]
DR RefSeq; NP_001078047.1; NM_001084578.1. [Q8LPQ9-1]
DR RefSeq; NP_001078048.1; NM_001084579.2. [Q8LPQ9-1]
DR RefSeq; NP_001323585.1; NM_001337028.1. [Q8LPQ9-2]
DR RefSeq; NP_181869.2; NM_129902.3. [Q8LPQ9-2]
DR PDB; 5KXF; X-ray; 2.70 A; A=433-565.
DR PDBsum; 5KXF; -.
DR AlphaFoldDB; Q8LPQ9; -.
DR SMR; Q8LPQ9; -.
DR BioGRID; 4278; 4.
DR IntAct; Q8LPQ9; 3.
DR STRING; 3702.AT2G43410.4; -.
DR iPTMnet; Q8LPQ9; -.
DR PaxDb; Q8LPQ9; -.
DR PRIDE; Q8LPQ9; -.
DR ProteomicsDB; 230040; -. [Q8LPQ9-1]
DR EnsemblPlants; AT2G43410.1; AT2G43410.1; AT2G43410. [Q8LPQ9-2]
DR EnsemblPlants; AT2G43410.2; AT2G43410.2; AT2G43410. [Q8LPQ9-1]
DR EnsemblPlants; AT2G43410.3; AT2G43410.3; AT2G43410. [Q8LPQ9-1]
DR EnsemblPlants; AT2G43410.4; AT2G43410.4; AT2G43410. [Q8LPQ9-1]
DR EnsemblPlants; AT2G43410.6; AT2G43410.6; AT2G43410. [Q8LPQ9-2]
DR GeneID; 818942; -.
DR Gramene; AT2G43410.1; AT2G43410.1; AT2G43410. [Q8LPQ9-2]
DR Gramene; AT2G43410.2; AT2G43410.2; AT2G43410. [Q8LPQ9-1]
DR Gramene; AT2G43410.3; AT2G43410.3; AT2G43410. [Q8LPQ9-1]
DR Gramene; AT2G43410.4; AT2G43410.4; AT2G43410. [Q8LPQ9-1]
DR Gramene; AT2G43410.6; AT2G43410.6; AT2G43410. [Q8LPQ9-2]
DR KEGG; ath:AT2G43410; -.
DR Araport; AT2G43410; -.
DR TAIR; locus:2058203; AT2G43410.
DR eggNOG; KOG0118; Eukaryota.
DR InParanoid; Q8LPQ9; -.
DR OMA; DINHIPR; -.
DR OrthoDB; 487634at2759; -.
DR PhylomeDB; Q8LPQ9; -.
DR PRO; PR:Q8LPQ9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8LPQ9; baseline and differential.
DR GO; GO:0000785; C:chromatin; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; ISS:TAIR.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0009553; P:embryo sac development; IGI:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0031048; P:heterochromatin assembly by small RNA; IMP:TAIR.
DR GO; GO:0006378; P:mRNA polyadenylation; IDA:UniProtKB.
DR GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:TAIR.
DR Gene3D; 3.30.70.330; -; 3.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR012921; SPOC_C.
DR Pfam; PF00076; RRM_1; 3.
DR Pfam; PF07744; SPOC; 1.
DR SMART; SM00360; RRM; 3.
DR SUPFAM; SSF54928; SSF54928; 3.
DR PROSITE; PS50102; RRM; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; Differentiation;
KW Flowering; Nucleus; Reference proteome; Repeat; RNA-binding; Transcription;
KW Transcription regulation.
FT CHAIN 1..901
FT /note="Flowering time control protein FPA"
FT /id="PRO_0000391778"
FT DOMAIN 18..90
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 95..166
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 206..281
FT /note="RRM 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 441..537
FT /note="SPOC"
FT REGION 343..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 655..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 655..686
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 696..716
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..324
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_038750"
FT VAR_SEQ 295..337
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_038751"
FT VAR_SEQ 325..351
FT /note="PLRGTNERSYNGAEYNDVVGKEPNWRR -> MSCLLSKTILVFTLNTMTLLV
FT RSQTGG (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_038752"
FT CONFLICT 110
FT /note="D -> G (in Ref. 6; BAH19726)"
FT /evidence="ECO:0000305"
FT CONFLICT 282
FT /note="E -> G (in Ref. 6; BAH19726)"
FT /evidence="ECO:0000305"
FT CONFLICT 494
FT /note="E -> G (in Ref. 6; BAH19726)"
FT /evidence="ECO:0000305"
FT CONFLICT 663
FT /note="H -> P (in Ref. 6; BAH19726)"
FT /evidence="ECO:0000305"
FT CONFLICT 687
FT /note="A -> V (in Ref. 6; BAH19726)"
FT /evidence="ECO:0000305"
FT CONFLICT 888
FT /note="Q -> R (in Ref. 5; AAM19833/AAO23594)"
FT /evidence="ECO:0000305"
FT STRAND 442..447
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 450..459
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 470..474
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 476..479
FT /evidence="ECO:0007829|PDB:5KXF"
FT HELIX 480..489
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 491..502
FT /evidence="ECO:0007829|PDB:5KXF"
FT HELIX 503..505
FT /evidence="ECO:0007829|PDB:5KXF"
FT HELIX 506..517
FT /evidence="ECO:0007829|PDB:5KXF"
FT TURN 518..520
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 521..526
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 532..536
FT /evidence="ECO:0007829|PDB:5KXF"
FT HELIX 541..544
FT /evidence="ECO:0007829|PDB:5KXF"
FT STRAND 552..560
FT /evidence="ECO:0007829|PDB:5KXF"
SQ SEQUENCE 901 AA; 99598 MW; 39E880D9D8FFF355 CRC64;
MALSMKPFRA DDSGFQSNNL WVGSLTPETT ESDLTELFGR YGDIDRITVY SSRGFAFIYY
RHVEEAVAAK EALQGANLNG SQIKIEYARP AKPCKSLWVG GIGPNVSKDD LEEEFSKFGK
IEDFRFLRER KTAFIDYYEM DDALQAKSMN GKPMGGSFLR VDFLRSQAPK KEQWAGSYDN
RNGNMNHKPQ YPHSYEDFKG DVQPSKVLWI GFPPTATQCN DEQILHNAMI LFGEIERVKS
YPSRNFALVE FRSAEEARQC KEGLQGRLFN NPRIKIMYSN DELPPEQDDT SFYSGMKRSR
TDMFNNDPSF VSSPHSTGIP GSMRPLRGTN ERSYNGAEYN DVVGKEPNWR RPSANGTGIL
PSPTGPGILP SPAQGTRRPM RSNPDSWEGY DPAQLVRESK RTRRDGSVDG FTPMGVDERS
FGRGSVAARP IRGPPDSDHI WRGMIAKGGT PVCCARCVPM GKGIETKLPE VVNCSARTDL
NMLAKHYAVA IGCEIVFFVP DREEDFASYT EFLRYLSSKD RAGVAKLDDG TTLFLVPPSD
FLTDVLQVTR QERLYGVVLK LPPPAVPVTA SYRQESQSNP LHYMDQARDS PANASHSLYP
PRENYIRGAP EHLTAASKPS VSEPLRIPNN AAPQAGVSLT PELLATLASI LPATSQPAAP
ESHQPMSGPS TVVSTAHQSN GLYNGEAPSQ AWKRGPQTVH DASNQSFQQY GNQYTPAGQL
PPPPSRYPPA SNNPNYTSGM VHGNMQYQSQ SVNMPQLSPL PNMPHNNYSM YTQGSSNHPV
SQPMVQQYQP EASMPNQNYG PIPSYQQANF HGVTTNQAQN LNPSQFQAAM QPPADKANLE
PQNQALRLQP MISGDGQGTT DGEVDKNQRY QSTLQFAANL LLQIQQKQQQ QSSGTPAGQG
P
