ID   ALCP_BACP3              Reviewed;         445 AA.
AC   P30145;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Sodium/proton-dependent alanine carrier protein {ECO:0000305};
GN   Name=acp {ECO:0000303|PubMed:1400476};
OS   Bacillus sp. (strain PS3).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=2334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=1400476; DOI=10.1016/s0021-9258(19)36661-x;
RA   Kamata H., Akiyama S., Morosawa H., Ohta T., Hamamoto T., Kambe T.,
RA   Kagawa Y., Hirata H.;
RT   "Primary structure of the alanine carrier protein of thermophilic bacterium
RT   PS3.";
RL   J. Biol. Chem. 267:21650-21655(1992).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND BLOCKED N-TERMINUS.
RC   STRAIN=PS3;
RX   PubMed=6469977; DOI=10.1016/s0021-9258(18)90559-4;
RA   Hirata H., Kambe T., Kagawa Y.;
RT   "A purified alanine carrier composed of a single polypeptide from
RT   thermophilic bacterium PS3 driven by either proton or sodium ion
RT   gradient.";
RL   J. Biol. Chem. 259:10653-10656(1984).
CC   -!- FUNCTION: Mediates the active transport of alanine, driven by either an
CC       H(+) or Na(+) gradient. {ECO:0000269|PubMed:6469977}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:6469977};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:6469977}.
CC   -!- SIMILARITY: Belongs to the alanine or glycine:cation symporter (AGCS)
CC       (TC 2.A.25) family. {ECO:0000305}.
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DR   EMBL; D12512; BAA02075.1; -; Genomic_DNA.
DR   AlphaFoldDB; P30145; -.
DR   SMR; P30145; -.
DR   TCDB; 2.A.25.1.2; the alanine or glycine:cation symporter (agcs) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015655; F:alanine:sodium symporter activity; IEA:InterPro.
DR   InterPro; IPR001463; Na/Ala_symport.
DR   PANTHER; PTHR30330; PTHR30330; 1.
DR   Pfam; PF01235; Na_Ala_symp; 1.
DR   PRINTS; PR00175; NAALASMPORT.
DR   TIGRFAMs; TIGR00835; agcS; 1.
DR   PROSITE; PS00873; NA_ALANINE_SYMP; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Cell membrane; Direct protein sequencing;
KW   Ion transport; Membrane; Sodium; Sodium transport; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..445
FT                   /note="Sodium/proton-dependent alanine carrier protein"
FT                   /id="PRO_0000161563"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        375..395
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   445 AA;  47804 MW;  B18C10B7F7BF5759 CRC64;
     MIRLVTMGKS SEAGVSSFQA LTMSLSGRIG VGNVAGTATG IAYGGPGAVF WMWVITFIGA
     ATAYVESTWR KFIKRNKTDN TVAVRRSTLK KALAGNGLRC SRAAIILSMA VLMPGIQANS
     IADSFSNAFG IPKLVTGIFV IAVLGFTIFG GVKRIAKTAE IVVPFMAVGY LFVAIAIIAA
     NIEKVPDVFG LIFKSAFGAD QVFGGILGSA VMWGVKRGLY ANEAGQGTGA HPAAAAEVSH
     PAKQGLVQAF SIYLDVFLVV TATALMILFT GQYNVINEKT GETIVEHLKG VEPGAGYTQA
     AVDTLFPGFG SAFIAIALFF FAFTTMYAYY YIAETNLAYL VRSEKRGTAF FALKLVFLAA
     TFYGTVKTAT TAWAMGDIGL GIMVWLNLIA ILLLFKPAYM ALKDYEEQLK QGKDPEFNAS
     KYGIKNAKFW ENGYKRWEEK KGKAL