ALCS_EMENI
ID ALCS_EMENI Reviewed; 262 AA.
AC Q460G9; C8VL21; Q5ARU9;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Protein alcS;
GN Name=alcS {ECO:0000312|EMBL:AAZ20475.1}; ORFNames=AN8981;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAZ20475.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16531087; DOI=10.1016/j.fgb.2005.12.008;
RA Flipphi M., Robellet X., Dequier E., Leschelle X., Felenbok B., Velot C.;
RT "Functional analysis of alcS, a gene of the alc cluster in Aspergillus
RT nidulans.";
RL Fungal Genet. Biol. 43:247-260(2006).
RN [2] {ECO:0000312|EMBL:EAA64313.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [3] {ECO:0000305, ECO:0000312|EMBL:CBF84523.1}
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [4] {ECO:0000305}
RP INDUCTION.
RX PubMed=8736527; DOI=10.1046/j.1365-2958.1996.5301061.x;
RA Fillinger S., Felenbok B.;
RT "A newly identified gene cluster in Aspergillus nidulans comprises five
RT novel genes localized in the alc region that are controlled both by the
RT specific transactivator AlcR and the general carbon-catabolite repressor
RT CreA.";
RL Mol. Microbiol. 20:475-488(1996).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16531087};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16531087}. Cell septum
CC {ECO:0000269|PubMed:16531087}.
CC -!- INDUCTION: By ethanol and ethylacetate. Basal level and ethanol-induced
CC expression repressed by acetate when grown on lactose. By ethyl methyl
CC ketone (EMK). Transcriptionally regulated by transcriptional activator
CC alcR and carbon catabolite repressor creA.
CC {ECO:0000269|PubMed:16531087, ECO:0000269|PubMed:8736527}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Does not affect growth on
CC ethanol, glycerol or acetate as a sole carbon source. Does not result
CC in phenotypes related to possible secretion of ammonia. Grows normally
CC on media with low concentrations of ethanol. No effect when screening
CC for allylalcohol and n-propanol toxicity. AlcA and alcS double mutant
CC has no difference in phenotype from alcA single mutant. FacA and alcS
CC double mutant does not differ from a single facA mutant in its ability
CC to grow on a medium with glycerol as a carbon source either alone or in
CC presence of ethanol. {ECO:0000269|PubMed:16531087}.
CC -!- SIMILARITY: Belongs to the acetate uptake transporter (AceTr) (TC
CC 2.A.96) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAA64313.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ076245; AAZ20475.1; -; Genomic_DNA.
DR EMBL; AACD01000168; EAA64313.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BN001307; CBF84523.1; -; Genomic_DNA.
DR RefSeq; XP_682250.1; XM_677158.1.
DR AlphaFoldDB; Q460G9; -.
DR SMR; Q460G9; -.
DR STRING; 227321.Q460G9; -.
DR TCDB; 2.A.96.2.1; the acetate uptake transporter (acetr) family.
DR EnsemblFungi; CBF84523; CBF84523; ANIA_08981.
DR EnsemblFungi; EAA64313; EAA64313; AN8981.2.
DR GeneID; 2868276; -.
DR KEGG; ani:AN8981.2; -.
DR VEuPathDB; FungiDB:AN8981; -.
DR eggNOG; ENOG502S179; Eukaryota.
DR HOGENOM; CLU_051062_4_0_1; -.
DR InParanoid; Q460G9; -.
DR OMA; IFMICAT; -.
DR OrthoDB; 1251190at2759; -.
DR Proteomes; UP000000560; Chromosome VII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0030428; C:cell septum; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:AspGD.
DR GO; GO:0015123; F:acetate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0019413; P:acetate biosynthetic process; IEP:AspGD.
DR GO; GO:0006068; P:ethanol catabolic process; IEP:AspGD.
DR InterPro; IPR030186; AlcS/PatA.
DR InterPro; IPR000791; Gpr1/Fun34/SatP.
DR PANTHER; PTHR31123:SF4; PTHR31123:SF4; 1.
DR Pfam; PF01184; Gpr1_Fun34_YaaH; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..262
FT /note="Protein alcS"
FT /id="PRO_0000393862"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 262 AA; 28014 MW; 836C337DED2E9B17 CRC64;
MTTEISNGEA KGHHLSTIPS SITLSAEQFE KLYLSPMMRQ QPSLARKVGN PTPLALGGFV
ITTTPLSCCL MAWRGSSGNG IAFIGPIIFL GGLLLLITSI LEFILGNTFP CVVFGTIGGF
WFAFAATMIP SFNAAAPYSS SATSTTAGLT SASFMNTYAF LFITMAVLML IFLLCATRTN
VVYTLIFLSL LLVFLLLSAG YWRLGEGDAA VGDRCIKGAG ASLFVASLLG FYLLIAQLFD
AVGLPITLPV GDMERFWPRS QK
