ID   ALC_BPT4                Reviewed;         167 AA.
AC   P04546;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   23-FEB-2022, entry version 82.
DE   RecName: Full=Protein alc;
DE   AltName: Full=Protein unf;
GN   Name=alc; Synonyms=unf;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6389256; DOI=10.1093/genetics/108.2.291;
RA   Kutter E.M., Drivdahl R., Rand K.;
RT   "Identification and characterization of the alc gene product of
RT   bacteriophage T4.";
RL   Genetics 108:291-304(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 55-136.
RX   PubMed=3782028; DOI=10.1128/jb.168.2.833-838.1986;
RA   Snyder L., Jorissen L.;
RT   "Molecular proof that bacteriophage T4 alc and unf genes are the same
RT   gene.";
RL   J. Bacteriol. 168:833-838(1986).
RN   [4]
RP   FUNCTION.
RX   PubMed=2185231; DOI=10.1128/jb.172.5.2716-2727.1990;
RA   Drivdahl R.H., Kutter E.M.;
RT   "Inhibition of transcription of cytosine-containing DNA in vitro by the alc
RT   gene product of bacteriophage T4.";
RL   J. Bacteriol. 172:2716-2727(1990).
RN   [5]
RP   FUNCTION.
RX   PubMed=8402894; DOI=10.1016/s0092-8674(05)80091-1;
RA   Kashlev M., Nudler E., Goldfarb A., White T., Kutter E.;
RT   "Bacteriophage T4 Alc protein: a transcription termination factor sensing
RT   local modification of DNA.";
RL   Cell 75:147-154(1993).
RN   [6]
RP   REVIEW.
RA   Kutter E.M., White T., Kashlev M., Uzan M., McKinney J., Guttman B.;
RL   (In) Karam J.D. (eds.);
RL   Molecular biology of bacteriophage T4, pp.357-368, American Society for
RL   Microbiology, Washington D.C. (1994).
CC   -!- FUNCTION: Participates in the host transcription shutoff by causing
CC       premature termination of transcription from host DNA. Acts as a site-
CC       specific termination factor that abolishes transcript elongation on
CC       cytosine-containing DNA but not on the 5-hydroxymethyl cytosine present
CC       in the viral DNA. Therefore inhibits as well transcription from other
CC       phages that contain cytosine in their DNA. {ECO:0000269|PubMed:2185231,
CC       ECO:0000269|PubMed:8402894}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X03193; CAA26950.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42532.1; -; Genomic_DNA.
DR   EMBL; M14731; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A04337; ZXBPAL.
DR   RefSeq; NP_049838.1; NC_000866.4.
DR   GeneID; 1258715; -.
DR   KEGG; vg:1258715; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039653; P:suppression by virus of host transcription; IMP:CACAO.
DR   InterPro; IPR020367; Host_transcript_inhib_Alc.
DR   Pfam; PF17527; ALC; 1.
PE   4: Predicted;
KW   Bacterial host gene expression shutoff by virus;
KW   Bacterial host transcription shutoff by virus; DNA-binding;
KW   Host gene expression shutoff by virus; Host-virus interaction;
KW   Reference proteome.
FT   CHAIN           1..167
FT                   /note="Protein alc"
FT                   /id="PRO_0000164915"
FT   CONFLICT        104
FT                   /note="C -> Y (in Ref. 1; CAA26950)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   167 AA;  18961 MW;  DCF76AD627598F06 CRC64;
     MDLQLITTEM VVEAYGDTTD GISVFKGNRR VGYITGLKKD LAKQVKRKTT IKEYRNRRLE
     QARDMLPDAV EEMKVFLENQ LAKYDCEVFI NQTQPNVHIN SCKCYIIVNP LTGKHRLGIS
     NPNRSASDMA EDVEACFKIS KSPAEHHILI NGLSQDDIVE VIKTLCM