FPR18_CAEEL
ID FPR18_CAEEL Reviewed; 432 AA.
AC G5EEB1; A0A2C9C3N0; G5EGC5; Q29XY6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=FMRFamide peptide receptor frpr-18 {ECO:0000312|WormBase:T19F4.1b};
DE AltName: Full=FLP2 receptor {ECO:0000303|PubMed:15809090};
GN Name=frpr-18 {ECO:0000312|WormBase:T19F4.1b};
GN ORFNames=T19F4.1 {ECO:0000312|WormBase:T19F4.1b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=15809090; DOI=10.1016/j.bbrc.2005.03.071;
RA Mertens I., Meeusen T., Janssen T., Nachman R., Schoofs L.;
RT "Molecular characterization of two G protein-coupled receptor splice
RT variants as FLP2 receptors in Caenorhabditis elegans.";
RL Biochem. Biophys. Res. Commun. 330:967-974(2005).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=24533288; DOI=10.1016/j.ijpddr.2012.10.002;
RA Larsen M.J., Lancheros E.R., Williams T., Lowery D.E., Geary T.G.,
RA Kubiak T.M.;
RT "Functional expression and characterization of the C. elegans G-protein-
RT coupled FLP-2 Receptor (T19F4.1) in mammalian cells and yeast.";
RL Int. J. Parasitol. Drugs Drug Resist. 3:1-7(2013).
RN [4] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=27585848; DOI=10.1534/genetics.116.192898;
RA Chen D., Taylor K.P., Hall Q., Kaplan J.M.;
RT "The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis
RT elegans Locomotion.";
RL Genetics 204:1151-1159(2016).
CC -!- FUNCTION: G-protein coupled receptor for flp-2 neuropeptides
CC (PubMed:15809090, PubMed:24533288). May act through the G(q) alpha type
CC of G proteins (PubMed:15809090, PubMed:24533288). Involved in mediating
CC arousal from the sleep-like state called lethargus, which occurs during
CC molting between larval and adult stages, in part by regulating touch
CC sensitivity, and working in concert with neuropeptide pdf-1
CC (PubMed:27585848). {ECO:0000269|PubMed:15809090,
CC ECO:0000269|PubMed:24533288, ECO:0000269|PubMed:27585848}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=b {ECO:0000312|WormBase:T19F4.1b};
CC IsoId=G5EEB1-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:T19F4.1a};
CC IsoId=G5EEB1-2; Sequence=VSP_061255, VSP_061257;
CC Name=c {ECO:0000312|WormBase:T19F4.1c};
CC IsoId=G5EEB1-3; Sequence=VSP_061254, VSP_061256, VSP_061258;
CC -!- TISSUE SPECIFICITY: Expressed in a subset of neurons in the head,
CC midbody, and tail, including AIY, ASI, BAG, URA, CAN, I6, PVQ, DVA,
CC RIM, and VC, and in the anal sphincter and intestinal muscles
CC (PubMed:27585848). Expression from the ASI neurons is involved in
CC promoting arousal (PubMed:27585848). {ECO:0000269|PubMed:27585848}.
CC -!- DISRUPTION PHENOTYPE: Decreased locomotion velocity (PubMed:27585848).
CC Restores normal quiescence during molting between larval and adult
CC stages, in neuropeptide receptor npr-1 mutant background
CC (PubMed:27585848). {ECO:0000269|PubMed:27585848}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAY16563.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY919614; AAY16563.1; ALT_SEQ; mRNA.
DR EMBL; AY919615; AAY16564.1; -; mRNA.
DR EMBL; BX284605; CCD68154.2; -; Genomic_DNA.
DR EMBL; BX284605; CCD68155.1; -; Genomic_DNA.
DR EMBL; BX284605; SOF58866.1; -; Genomic_DNA.
DR RefSeq; NP_504746.1; NM_072345.3.
DR AlphaFoldDB; G5EEB1; -.
DR SMR; G5EEB1; -.
DR STRING; 6239.T19F4.1b; -.
DR PaxDb; G5EEB1; -.
DR EnsemblMetazoa; T19F4.1a.1; T19F4.1a.1; WBGene00020586. [G5EEB1-2]
DR EnsemblMetazoa; T19F4.1b.1; T19F4.1b.1; WBGene00020586. [G5EEB1-1]
DR EnsemblMetazoa; T19F4.1c.1; T19F4.1c.1; WBGene00020586. [G5EEB1-3]
DR GeneID; 179076; -.
DR KEGG; cel:CELE_T19F4.1; -.
DR CTD; 179076; -.
DR WormBase; T19F4.1a; CE28679; WBGene00020586; frpr-18.
DR WormBase; T19F4.1b; CE29349; WBGene00020586; frpr-18.
DR WormBase; T19F4.1c; CE52203; WBGene00020586; frpr-18.
DR eggNOG; KOG3656; Eukaryota.
DR HOGENOM; CLU_009579_24_8_1; -.
DR InParanoid; G5EEB1; -.
DR OMA; HWCYLIV; -.
DR OrthoDB; 994073at2759; -.
DR PhylomeDB; G5EEB1; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00020586; Expressed in larva and 2 other tissues.
DR ExpressionAtlas; G5EEB1; baseline and differential.
DR GO; GO:0005887; C:integral component of plasma membrane; IMP:UniProtKB.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IEA:InterPro.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IMP:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IMP:UniProtKB.
DR GO; GO:0050714; P:positive regulation of protein secretion; IGI:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IMP:UniProtKB.
DR GO; GO:0030431; P:sleep; IGI:UniProtKB.
DR InterPro; IPR019427; 7TM_GPCR_serpentine_rcpt_Srw.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF10324; 7TM_GPCR_Srw; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; G-protein coupled receptor; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..432
FT /note="FMRFamide peptide receptor frpr-18"
FT /id="PRO_0000454195"
FT TOPO_DOM 1..8
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 9..29
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..42
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 43..63
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..84
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 85..105
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..176
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 177..197
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 226..246
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..271
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 272..292
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..432
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 328..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..411
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 219..260
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061254"
FT VAR_SEQ 347..409
FT /note="SRSSDVLLKPIYMQKRSERFSSEYNERTCKHLAPFEEHKLPKLPSEKRKKKL
FT HKMSAVEHRGM -> RKPILNAHLSEPLIGRSSQTNLHAEKKRTLLVGVQRTNLQTPCS
FT FRRAQITKIAEREEEEETT (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_061255"
FT VAR_SEQ 348..411
FT /note="RSSDVLLKPIYMQKRSERFSSEYNERTCKHLAPFEEHKLPKLPSEKRKKKLH
FT KMSAVEHRGMPE -> YRKPILNAHLSEPLIGRSSQTNLHAEKKRTLLVGVQRTNLQTP
FT CSFRRAQITKIAEREEEEETT (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061256"
FT VAR_SEQ 410..432
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_061257"
FT VAR_SEQ 412..432
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061258"
SQ SEQUENCE 432 AA; 49764 MW; 86BC08D04BB34B12 CRC64;
MESQQLMACA ILVIVLVGIF GNSLSFILFS RPHMRSSSVN VLLCALSFFD FSLLTLSIPI
FVIPNLDLWA NDLSLSTYMA YILKLIYPIN LMMQTCSVYI MVMITLERWV AVCRPLQVRV
WCTPRKSRNA ILVIIVSAFL YNFVRFFEYR FVVTESGALY EKWLRDPGKH RWYYVGYYTI
LYIVTHFLVP FSVMAFANGH VIVAMCKLSK TRQMLTRQQQ REQSTTVMLL IVTFVFAICN
TLPFLLNVSE SIFPTLFQDE STRGLAYWLN DLSNLLVVLN SGTTFIIYFT FSEKYRQTLV
FILKNGCCAT VSDYNNYTAM SRTASMRISS ETGGQIQRQG SKMSNSSRSS DVLLKPIYMQ
KRSERFSSEY NERTCKHLAP FEEHKLPKLP SEKRKKKLHK MSAVEHRGMP EITITFSEDL
PDGEPDSPCQ PC
