FRA12_FRAAN
ID FRA12_FRAAN Reviewed; 160 AA.
AC D0E0C6;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=Major strawberry allergen Fra a 1-2 {ECO:0000305};
DE AltName: Full=Class 10 plant pathogenesis-related protein Fra a 1.02 {ECO:0000305};
DE Short=PR10-related protein Fra a 1.02 {ECO:0000305};
DE AltName: Allergen=Fra a 1 {ECO:0000305};
GN Name=FRAA2 {ECO:0000303|PubMed:19969523};
OS Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC Fragaria.
OX NCBI_TaxID=3747;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=19969523; DOI=10.1093/mp/ssp087;
RA Munoz C., Hoffmann T., Escobar N.M., Ludemann F., Botella M.A.,
RA Valpuesta V., Schwab W.;
RT "The strawberry fruit Fra a allergen functions in flavonoid biosynthesis.";
RL Mol. Plant 3:113-124(2010).
RN [2]
RP FUNCTION.
RX PubMed=24133217; DOI=10.1074/jbc.m113.501528;
RA Casanal A., Zander U., Munoz C., Dupeux F., Luque I., Botella M.A.,
RA Schwab W., Valpuesta V., Marquez J.A.;
RT "The strawberry pathogenesis-related 10 (PR-10) Fra a proteins control
RT flavonoid biosynthesis by binding to metabolic intermediates.";
RL J. Biol. Chem. 288:35322-35332(2013).
RN [3]
RP ALLERGEN.
RX PubMed=27086707; DOI=10.1021/acs.jafc.6b00488;
RA Franz-Oberdorf K., Eberlein B., Edelmann K., Huecherig S., Besbes F.,
RA Darsow U., Ring J., Schwab W.;
RT "Fra a 1.02 is the most potent isoform of the Bet v 1-like allergen in
RT strawberry fruit.";
RL J. Agric. Food Chem. 64:3688-3696(2016).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=28574483; DOI=10.3390/ijms18061186;
RA Ishibashi M., Yoshikawa H., Uno Y.;
RT "Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening
RT Controlled by Exogenous Auxin.";
RL Int. J. Mol. Sci. 18:0-0(2017).
RN [5]
RP SUBUNIT, AND INTERACTION WITH AP.
RX PubMed=28656626; DOI=10.1002/prot.25343;
RA Franz-Oberdorf K., Langer A., Strasser R., Isono E., Ranftl Q.L.,
RA Wunschel C., Schwab W.;
RT "Physical interaction between the strawberry allergen Fra a 1 and an
RT associated partner FaAP: Interaction of Fra a 1 proteins and FaAP.";
RL Proteins 85:1891-1901(2017).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-160.
RX PubMed=27050125; DOI=10.1107/s2059798316000954;
RA Zander U., Hoffmann G., Cornaciu I., Marquette J.-P., Papp G., Landret C.,
RA Seroul G., Sinoir J., Roewer M., Felisaz F., Rodriguez-Puente S.,
RA Mariaule V., Murphy P., Mathieu M., Cipriani F., Marquez J.A.;
RT "Automated harvesting and processing of protein crystals through laser
RT photoablation.";
RL Acta Crystallogr. D 72:454-466(2016).
CC -!- FUNCTION: Involved in the control of flavonoid biosynthesis in fruits,
CC probably by binding directly to natural flavonoids (By similarity).
CC Binds the natural flavonoid myricetin with affinities in the low
CC micromolar range (PubMed:24133217). {ECO:0000250|UniProtKB:D0E0C7,
CC ECO:0000269|PubMed:24133217}.
CC -!- SUBUNIT: Monomer (PubMed:28656626). Interacts with AP
CC (PubMed:28656626). {ECO:0000269|PubMed:28656626}.
CC -!- TISSUE SPECIFICITY: Highly expressed in ripe red fruits
CC (PubMed:19969523, PubMed:28574483). Expressed in roots and white fruits
CC (PubMed:19969523). Expressed at low levels in open flowers
CC (PubMed:19969523). {ECO:0000269|PubMed:19969523,
CC ECO:0000269|PubMed:28574483}.
CC -!- ALLERGEN: May cause an allergic reaction in human (PubMed:27086707).
CC Binds to IgE of patients allergic to birch Bet v 1 (PubMed:27086707).
CC Causes degranulation of basophils sensitized with IgE of patients
CC allergic to birch Bet v 1 (PubMed:27086707).
CC {ECO:0000269|PubMed:27086707}.
CC -!- SIMILARITY: Belongs to the BetVI family. {ECO:0000305}.
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DR EMBL; GQ148818; ACX47057.1; -; mRNA.
DR PDB; 5AMW; X-ray; 1.90 A; A/B=2-160.
DR PDB; 6ST8; X-ray; 2.04 A; A/B=2-160.
DR PDB; 6ST9; X-ray; 1.97 A; A/B=2-160.
DR PDB; 6STA; X-ray; 2.19 A; A/B=2-160.
DR PDB; 6STB; X-ray; 2.27 A; A/B=2-160.
DR PDBsum; 5AMW; -.
DR PDBsum; 6ST8; -.
DR PDBsum; 6ST9; -.
DR PDBsum; 6STA; -.
DR PDBsum; 6STB; -.
DR AlphaFoldDB; D0E0C6; -.
DR SMR; D0E0C6; -.
DR Allergome; 2124; Fra a 1.
DR GO; GO:0010427; F:abscisic acid binding; IEA:InterPro.
DR GO; GO:0004864; F:protein phosphatase inhibitor activity; IEA:InterPro.
DR GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR Gene3D; 3.30.530.20; -; 1.
DR InterPro; IPR000916; Bet_v_I/MLP.
DR InterPro; IPR024949; Bet_v_I_allergen.
DR InterPro; IPR023393; START-like_dom_sf.
DR Pfam; PF00407; Bet_v_1; 1.
DR PRINTS; PR00634; BETALLERGEN.
DR PROSITE; PS00451; PATHOGENESIS_BETVI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Flavonoid biosynthesis;
KW Pathogenesis-related protein; Plant defense.
FT CHAIN 1..160
FT /note="Major strawberry allergen Fra a 1-2"
FT /id="PRO_0000447013"
FT STRAND 2..14
FT /evidence="ECO:0007829|PDB:5AMW"
FT HELIX 16..23
FT /evidence="ECO:0007829|PDB:5AMW"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:5AMW"
FT HELIX 27..34
FT /evidence="ECO:0007829|PDB:5AMW"
FT TURN 36..38
FT /evidence="ECO:0007829|PDB:5AMW"
FT STRAND 39..50
FT /evidence="ECO:0007829|PDB:5AMW"
FT STRAND 54..59
FT /evidence="ECO:0007829|PDB:5AMW"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:5AMW"
FT STRAND 67..76
FT /evidence="ECO:0007829|PDB:5AMW"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:5AMW"
FT STRAND 81..89
FT /evidence="ECO:0007829|PDB:5AMW"
FT STRAND 94..108
FT /evidence="ECO:0007829|PDB:5AMW"
FT STRAND 113..126
FT /evidence="ECO:0007829|PDB:5AMW"
FT HELIX 131..154
FT /evidence="ECO:0007829|PDB:5AMW"
FT TURN 156..159
FT /evidence="ECO:0007829|PDB:5AMW"
SQ SEQUENCE 160 AA; 17499 MW; 31122152CB57C6E1 CRC64;
MGVFTYETEF TSVIPPPRLF KAFILDADNL IPKIAPQAVK CAEIIEGDGG VGTIKKITFG
EGSQFGSVTH KIDGIDKENF VYSYSLIEGD ALSDKIEKIS YETKLVSSSD GGSIIKSTSN
YHTKGDVEIK EEHVKAGKEK ASHLFKLVEG YLLANPNEYC
