FRA1E_FRAAN
ID FRA1E_FRAAN Reviewed; 160 AA.
AC Q256S2;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2006, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Major strawberry allergen Fra a 1-E {ECO:0000303|PubMed:16945416};
DE AltName: Full=Class 10 plant pathogenesis-related protein Fra a 1.01 {ECO:0000305};
DE Short=PR10-related protein Fra a 1.01 {ECO:0000305};
DE AltName: Full=Fra a 1.01E {ECO:0000305};
DE AltName: Allergen=Fra a 1 {ECO:0000305};
GN Name=FRAA1E.1 {ECO:0000303|PubMed:16945416};
GN Synonyms=FRAA1E.2 {ECO:0000303|PubMed:16945416};
OS Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC Fragaria.
OX NCBI_TaxID=3747;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Leaf;
RX PubMed=16945416; DOI=10.1016/j.molimm.2006.06.004;
RA Musidlowska-Persson A., Alm R., Emanuelsson C.;
RT "Cloning and sequencing of the Bet v 1-homologous allergen Fra a 1 in
RT strawberry (Fragaria ananassa) shows the presence of an intron and little
RT variability in amino acid sequence.";
RL Mol. Immunol. 44:1245-1252(2007).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19969523; DOI=10.1093/mp/ssp087;
RA Munoz C., Hoffmann T., Escobar N.M., Ludemann F., Botella M.A.,
RA Valpuesta V., Schwab W.;
RT "The strawberry fruit Fra a allergen functions in flavonoid biosynthesis.";
RL Mol. Plant 3:113-124(2010).
RN [3]
RP ALLERGEN.
RX PubMed=27086707; DOI=10.1021/acs.jafc.6b00488;
RA Franz-Oberdorf K., Eberlein B., Edelmann K., Huecherig S., Besbes F.,
RA Darsow U., Ring J., Schwab W.;
RT "Fra a 1.02 is the most potent isoform of the Bet v 1-like allergen in
RT strawberry fruit.";
RL J. Agric. Food Chem. 64:3688-3696(2016).
RN [4]
RP INTERACTION WITH AP.
RX PubMed=28656626; DOI=10.1002/prot.25343;
RA Franz-Oberdorf K., Langer A., Strasser R., Isono E., Ranftl Q.L.,
RA Wunschel C., Schwab W.;
RT "Physical interaction between the strawberry allergen Fra a 1 and an
RT associated partner FaAP: Interaction of Fra a 1 proteins and FaAP.";
RL Proteins 85:1891-1901(2017).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=22913709; DOI=10.1042/bsr20120058;
RA Seutter von Loetzen C., Schweimer K., Schwab W., Rosch P.,
RA Hartl-Spiegelhauer O.;
RT "Solution structure of the strawberry allergen Fra a 1.";
RL Biosci. Rep. 32:567-575(2012).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 2-160, AND SUBUNIT.
RX PubMed=24133217; DOI=10.1074/jbc.m113.501528;
RA Casanal A., Zander U., Munoz C., Dupeux F., Luque I., Botella M.A.,
RA Schwab W., Valpuesta V., Marquez J.A.;
RT "The strawberry pathogenesis-related 10 (PR-10) Fra a proteins control
RT flavonoid biosynthesis by binding to metabolic intermediates.";
RL J. Biol. Chem. 288:35322-35332(2013).
CC -!- FUNCTION: Involved in the control of flavonoid biosynthesis in fruits,
CC probably by binding directly to natural flavonoids (PubMed:19969523).
CC Binds the natural flavonoid quercetin-3-O-glucuronide with affinities
CC in the low micromolar range (PubMed:24133217).
CC {ECO:0000269|PubMed:19969523, ECO:0000269|PubMed:24133217}.
CC -!- SUBUNIT: Monomer (PubMed:24133217). Interacts with AP
CC (PubMed:28656626). {ECO:0000269|PubMed:24133217,
CC ECO:0000269|PubMed:28656626}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots (PubMed:19969523).
CC Expressed in open flowers (PubMed:19969523). Expressed at low levels in
CC leaves, flower buds and fruits (PubMed:19969523).
CC {ECO:0000269|PubMed:19969523}.
CC -!- ALLERGEN: May cause an allergic reaction in human (PubMed:27086707).
CC Binds to IgE of patients allergic to birch Bet v 1 (PubMed:27086707).
CC Causes degranulation of basophils sensitized with IgE of patients
CC allergic to birch Bet v 1 (PubMed:27086707).
CC {ECO:0000269|PubMed:27086707}.
CC -!- MISCELLANEOUS: Fruits silencing FRAA3 exhibit loss of pigmentation in
CC mature fruits due to the lack of anthocyanin biosynthesis.
CC {ECO:0000269|PubMed:19969523}.
CC -!- SIMILARITY: Belongs to the BetVI family. {ECO:0000305}.
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DR EMBL; AM236319; CAJ85645.1; -; Genomic_DNA.
DR EMBL; AM236320; CAJ85646.1; -; Genomic_DNA.
DR PDB; 2LPX; NMR; -; A=1-160.
DR PDB; 4C9C; X-ray; 2.20 A; A/B=2-160.
DR PDB; 4C9I; X-ray; 3.10 A; A/B/C/D/E/F=2-160.
DR PDBsum; 2LPX; -.
DR PDBsum; 4C9C; -.
DR PDBsum; 4C9I; -.
DR AlphaFoldDB; Q256S2; -.
DR SMR; Q256S2; -.
DR Allergome; 2124; Fra a 1.
DR GO; GO:0010427; F:abscisic acid binding; IEA:InterPro.
DR GO; GO:0097243; F:flavonoid binding; IMP:UniProtKB.
DR GO; GO:0004864; F:protein phosphatase inhibitor activity; IEA:InterPro.
DR GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR Gene3D; 3.30.530.20; -; 1.
DR InterPro; IPR000916; Bet_v_I/MLP.
DR InterPro; IPR024949; Bet_v_I_allergen.
DR InterPro; IPR023393; START-like_dom_sf.
DR Pfam; PF00407; Bet_v_1; 1.
DR PRINTS; PR00634; BETALLERGEN.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Flavonoid biosynthesis;
KW Pathogenesis-related protein; Plant defense.
FT CHAIN 1..160
FT /note="Major strawberry allergen Fra a 1-E"
FT /id="PRO_0000447012"
FT STRAND 2..14
FT /evidence="ECO:0007829|PDB:4C9C"
FT HELIX 16..23
FT /evidence="ECO:0007829|PDB:4C9C"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:4C9C"
FT HELIX 27..34
FT /evidence="ECO:0007829|PDB:4C9C"
FT TURN 36..38
FT /evidence="ECO:0007829|PDB:4C9C"
FT STRAND 40..50
FT /evidence="ECO:0007829|PDB:4C9C"
FT STRAND 54..59
FT /evidence="ECO:0007829|PDB:4C9C"
FT STRAND 61..65
FT /evidence="ECO:0007829|PDB:2LPX"
FT STRAND 66..76
FT /evidence="ECO:0007829|PDB:4C9C"
FT TURN 77..80
FT /evidence="ECO:0007829|PDB:4C9C"
FT STRAND 81..89
FT /evidence="ECO:0007829|PDB:4C9C"
FT STRAND 94..107
FT /evidence="ECO:0007829|PDB:4C9C"
FT STRAND 113..126
FT /evidence="ECO:0007829|PDB:4C9C"
FT HELIX 131..154
FT /evidence="ECO:0007829|PDB:4C9C"
SQ SEQUENCE 160 AA; 17766 MW; 4DCADC67CD607B5B CRC64;
MGVYTYENEF TSDIPAPKLF KAFVLDADNL IPKIAPQAVK CAEILEGDGG PGTIKKITFG
EGSHYGYVKH KIHSIDKVNH TYSYSLIEGD ALSENIEKID YETKLVSAPH GGTIIKTTSK
YHTKGDVEIK EEHVKAGKEK AAHLFKLIEG YLKDHPSEYN
