ID   FRD_BPT5                Reviewed;         177 AA.
AC   Q6QGJ4;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Dihydrofolate reductase {ECO:0000312|EMBL:AAU05230.1};
DE            EC=1.5.1.3 {ECO:0000269|PubMed:4863042};
GN   Name=frd {ECO:0000312|EMBL:AAS77139.1};
GN   Synonyms=B3-frd {ECO:0000312|EMBL:AAX12025.1},
GN   dhfR {ECO:0000312|EMBL:AAU05230.1};
GN   ORFNames=T5.093 {ECO:0000312|EMBL:AAS77139.1},
GN   T5p091 {ECO:0000312|EMBL:AAU05230.1};
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12025.1};
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=St0 deletion mutant;
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=4863042; DOI=10.1016/s0021-9258(18)95782-0;
RA   Mathews C.K.;
RT   "Evidence that bacteriophage-induced dihydrofolate reductase in a viral
RT   gene product.";
RL   J. Biol. Chem. 242:4083-4086(1967).
RN   [5]
RP   FUNCTION.
RX   PubMed=392233; DOI=10.1007/bf00397233;
RA   Peter G., Haenggi U.J., Zachau H.G.;
RT   "Localization of the dihydrofolate reductase gene on the physical map of
RT   bacteriophage T5.";
RL   Mol. Gen. Genet. 175:333-341(1979).
CC   -!- FUNCTION: Provides the tetrahydrofolates necessary for the synthesis of
CC       nucleotides and amino acids. Bacteriophage T5 induces high levels of
CC       dihydrofolate reductase in the host cell, probably for the viral
CC       replication. {ECO:0000269|PubMed:392233, ECO:0000269|PubMed:4863042}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate +
CC         H(+) + NADPH; Xref=Rhea:RHEA:15009, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57451, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.5.1.3; Evidence={ECO:0000305|PubMed:4863042};
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305|PubMed:15661140}.
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DR   EMBL; AY543070; AAS77139.1; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05230.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX12025.1; -; Genomic_DNA.
DR   RefSeq; YP_006921.1; NC_005859.1.
DR   SMR; Q6QGJ4; -.
DR   GeneID; 2777595; -.
DR   KEGG; vg:2777595; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   Proteomes; UP000002503; Genome.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IDA:UniProtKB.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.430.10; -; 1.
DR   InterPro; IPR024072; DHFR-like_dom_sf.
DR   SUPFAM; SSF53597; SSF53597; 1.
PE   1: Evidence at protein level;
KW   DNA replication; NADP; Oxidoreductase; Reference proteome;
KW   Viral DNA replication.
FT   CHAIN           1..177
FT                   /note="Dihydrofolate reductase"
FT                   /id="PRO_0000435561"
SQ   SEQUENCE   177 AA;  19776 MW;  CC1C3E49143BB56D CRC64;
     MITAMYAVGP NGEFGLRGKL PWGSFKEELD AFYSQLDVLN PDNIIIGAGT YLALPYAVRE
     RMIGASDLFI RADRPLPDDI THDIYTPISM IGDTLPTFLK DQQTVVLGGA NLLLEMYQHG
     HIESAFVSTI FSEQKLEADT HLDSMILDYN YESTRLVYAV GANSDNSLRF VQELVTY