ID   FRE2_NYCIN              Reviewed;          15 AA.
AC   P82022;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   29-SEP-2021, entry version 43.
DE   RecName: Full=Frenatin-2 {ECO:0000303|PubMed:9225251};
OS   Nyctimystes infrafrenatus (White-lipped tree frog) (Litoria infrafrenata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae;
OC   Nyctimystes.
OX   NCBI_TaxID=61195;
RN   [1]
RP   PROTEIN SEQUENCE, AMIDATION AT LEU-15, MASS SPECTROMETRY, SUBCELLULAR
RP   LOCATION, AND SYNTHESIS.
RC   TISSUE=Skin secretion;
RX   PubMed=9225251; DOI=10.1002/psc.60;
RA   Raftery M.J., Waugh R.J., Bowie J.H., Wallace J.C., Tyler M.J.;
RT   "The structures of the frenatin peptides from the skin secretion of the
RT   giant tree frog Litoria infrafrenata.";
RL   J. Pept. Sci. 2:117-124(1996).
CC   -!- FUNCTION: Antimicrobial peptide with selective activity. Is only active
CC       against Micrococcus luteus (MIMC=50 ug/ml) and not against Bacillus
CC       cereus, Escherichia coli, Leuconostoc mesenteroides, Micrococcus
CC       luteus, Pastewella haemolytica, Staphylococcus aureus, Streptococcus
CC       faecalis and Streptococcus uberis. {ECO:0000269|PubMed:9225251}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9225251}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:9225251}.
CC   -!- MASS SPECTROMETRY: Mass=1423; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:9225251};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Frenatin subfamily. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=02007";
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..15
FT                   /note="Frenatin-2"
FT                   /evidence="ECO:0000269|PubMed:9225251"
FT                   /id="PRO_0000043799"
FT   MOD_RES         15
FT                   /note="Leucine amide"
FT                   /evidence="ECO:0000269|PubMed:9225251"
SQ   SEQUENCE   15 AA;  1425 MW;  06ED4B7BB1650CBF CRC64;
     GLLGTLGNLL NGLGL