ID   FRE3_NYCIN              Reviewed;          68 AA.
AC   P56249; Q571V5;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Frenatin-3 {ECO:0000303|PubMed:15996792, ECO:0000303|PubMed:9225251};
DE   Flags: Precursor;
OS   Nyctimystes infrafrenatus (White-lipped tree frog) (Litoria infrafrenata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae;
OC   Nyctimystes.
OX   NCBI_TaxID=61195;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-68, MASS SPECTROMETRY,
RP   AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin;
RX   PubMed=15996792; DOI=10.1016/j.peptides.2005.05.019;
RA   Zhou M., Chen T., Walker B., Shaw C.;
RT   "Novel frenatins from the skin of the Australasian giant white-lipped tree
RT   frog, Litoria infrafrenata: cloning of precursor cDNAs and identification
RT   in defensive skin secretion.";
RL   Peptides 26:2445-2451(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 47-68, FUNCTION, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=9225251; DOI=10.1002/psc.60;
RA   Raftery M.J., Waugh R.J., Bowie J.H., Wallace J.C., Tyler M.J.;
RT   "The structures of the frenatin peptides from the skin secretion of the
RT   giant tree frog Litoria infrafrenata.";
RL   J. Pept. Sci. 2:117-124(1996).
RN   [3]
RP   FUNCTION.
RX   PubMed=11784303; DOI=10.1046/j.0014-2956.2002.02630.x;
RA   Doyle J., Llewellyn L.E., Brinkworth C.S., Bowie J.H., Wegener K.L.,
RA   Rozek T., Wabnitz P.A., Wallace J.C., Tyler M.J.;
RT   "Amphibian peptides that inhibit neuronal nitric oxide synthase. Isolation
RT   of lesuerin from the skin secretion of the Australian stony creek frog
RT   Litoria lesueuri.";
RL   Eur. J. Biochem. 269:100-109(2002).
RN   [4]
RP   STRUCTURE BY NMR OF 47-68.
RX   PubMed=14579314; DOI=10.1002/bip.10524;
RA   Brinkworth C.S., Carver J.A., Wegener K.L., Doyle J., Llewellyn L.E.,
RA   Bowie J.H.;
RT   "The solution structure of frenatin 3, a neuronal nitric oxide synthase
RT   inhibitor from the giant tree frog, Litoria infrafrenata.";
RL   Biopolymers 70:424-434(2003).
CC   -!- FUNCTION: Antimicrobial peptide with activity against both Gram-
CC       positive and Gram-negative bacteria (PubMed:9225251). Antibacterial
CC       activities have been tested against Bacillus cereus (MIC=12.5 ug/ml),
CC       Escherichia coli (MIC=50 ug/ml), Leuconostoc mesenteroides (MIC=25
CC       ug/ml), Micrococcus luteus (MIC=1.5 ug/ml), Pastewella haemolytica
CC       (MIC=0.8 ug/ml), Staphylococcus aureus (MIC