ID   FRE4_NYCIN              Reviewed;          24 AA.
AC   P82023;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=Frenatin-4 {ECO:0000303|PubMed:9225251};
OS   Nyctimystes infrafrenatus (White-lipped tree frog) (Litoria infrafrenata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae;
OC   Nyctimystes.
OX   NCBI_TaxID=61195;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   TISSUE=Skin secretion;
RX   PubMed=9225251; DOI=10.1002/psc.60;
RA   Raftery M.J., Waugh R.J., Bowie J.H., Wallace J.C., Tyler M.J.;
RT   "The structures of the frenatin peptides from the skin secretion of the
RT   giant tree frog Litoria infrafrenata.";
RL   J. Pept. Sci. 2:117-124(1996).
CC   -!- FUNCTION: Very weak antimicrobial peptide since it does not show
CC       activity below 100 ug/ml against Bacillus cereus, Escherichia coli,
CC       Leuconostoc mesenteroides, Micrococcus luteus, Pastewella haemolytica,
CC       Staphylococcus aureus, Streptococcus faecalis and Streptococcus uberis.
CC       {ECO:0000269|PubMed:9225251}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9225251}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:9225251}.
CC   -!- MASS SPECTROMETRY: Mass=2493; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:9225251};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Frenatin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P82023; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..24
FT                   /note="Frenatin-4"
FT                   /evidence="ECO:0000269|PubMed:9225251"
FT                   /id="PRO_0000043801"
SQ   SEQUENCE   24 AA;  2495 MW;  6986D063947805A1 CRC64;
     GFLDKLKKGA SDFANALVNS IKGT