ID   FRHB_METBF              Reviewed;         291 AA.
AC   P80490; O33168; Q46FB1;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2006, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Coenzyme F420 hydrogenase subunit beta;
DE            EC=1.12.98.1;
DE   AltName: Full=8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta;
DE            Short=FRH;
GN   Name=frhB; OrderedLocusNames=Mbar_A0449;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9477253; DOI=10.1007/s002030050561;
RA   Vaupel M., Thauer R.K.;
RT   "Two F420-reducing hydrogenases in Methanosarcina barkeri.";
RL   Arch. Microbiol. 169:201-205(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-25.
RX   PubMed=8521835; DOI=10.1111/j.1432-1033.1995.727_3.x;
RA   Michel R., Massanz C., Kostka S., Richter M., Fiebig K.;
RT   "Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive
RT   hydrogenase from Methanosarcina barkeri Fusaro.";
RL   Eur. J. Biochem. 233:727-735(1995).
CC   -!- FUNCTION: Reduces the physiological low-potential two-electron acceptor
CC       coenzyme F420, and the artificial one-electron acceptor methylviologen.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + H2 + oxidized coenzyme F420-(gamma-L-Glu)(n) = reduced
CC         coenzyme F420-(gamma-L-Glu)(n); Xref=Rhea:RHEA:23760, Rhea:RHEA-
CC         COMP:12939, Rhea:RHEA-COMP:14378, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:133980, ChEBI:CHEBI:139511;
CC         EC=1.12.98.1;
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC   -!- COFACTOR:
CC       Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SUBUNIT: Pentamer of two alpha chains, two beta chains and a gamma
CC       chain.
CC   -!- SIMILARITY: Belongs to the FrhB family. {ECO:0000305}.
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DR   EMBL; Y13763; CAA74093.1; -; Genomic_DNA.
DR   EMBL; CP000099; AAZ69431.1; -; Genomic_DNA.
DR   PIR; S63484; S63484.
DR   RefSeq; WP_011305483.1; NC_007355.1.
DR   AlphaFoldDB; P80490; -.
DR   SMR; P80490; -.
DR   STRING; 269797.Mbar_A0449; -.
DR   EnsemblBacteria; AAZ69431; AAZ69431; Mbar_A0449.
DR   GeneID; 3624325; -.
DR   KEGG; mba:Mbar_A0449; -.
DR   eggNOG; arCOG02651; Archaea.
DR   HOGENOM; CLU_037958_0_0_2; -.
DR   OMA; REYGCEK; -.
DR   OrthoDB; 49654at2157; -.
DR   BioCyc; MetaCyc:MON-12648; -.
DR   BRENDA; 1.12.98.1; 3250.
DR   GO; GO:0050454; F:coenzyme F420 hydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR017679; FrhB_archaea.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   PANTHER; PTHR31332; PTHR31332; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
DR   TIGRFAMs; TIGR03289; frhB; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Iron; Iron-sulfur; Metal-binding;
KW   Oxidoreductase.
FT   CHAIN           1..291
FT                   /note="Coenzyme F420 hydrogenase subunit beta"
FT                   /id="PRO_0000159225"
SQ   SEQUENCE   291 AA;  32281 MW;  4061CC460D39EC63 CRC64;
     MIEDPYLGKY VTCVSARSTD KEILKKAQDG GIATALMVYA LEEGFIDGTI VAGEGDKPWQ
     PKPEVAMTRE EILKARGTRY SISPQISWLK EATRSFGLDK VGVTGVCCQM QAVRKAQLYP
     INMRDVPGKV AFTVGLFCME NFPYKSLQSI VEDHANQSLG SVKKMEITKG KFWVYTERGN
     VATVPLKATH KYEQPGCHVC LDYVSNLADI STGSVGSPDG WSTVFIRTKV GNEIWSKAVA
     DGMFETKPIE EVKPGLDLLR KLAKEKIEKN QKTVEERKTF GVNKGLRNPY A