ID   FRHB_METJA              Reviewed;         287 AA.
AC   Q60341;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Coenzyme F420 hydrogenase subunit beta;
DE            EC=1.12.98.1;
DE   AltName: Full=8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta;
DE            Short=FRH;
GN   Name=frhB; OrderedLocusNames=MJ0032;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: Reduces the physiological low-potential two-electron acceptor
CC       coenzyme F420, and the artificial one-electron acceptor methylviologen.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + H2 + oxidized coenzyme F420-(gamma-L-Glu)(n) = reduced
CC         coenzyme F420-(gamma-L-Glu)(n); Xref=Rhea:RHEA:23760, Rhea:RHEA-
CC         COMP:12939, Rhea:RHEA-COMP:14378, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:133980, ChEBI:CHEBI:139511;
CC         EC=1.12.98.1;
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000250};
CC   -!- COFACTOR:
CC       Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC         Evidence={ECO:0000250};
CC       Note=There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.
CC       {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the FrhB family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98011.1; -; Genomic_DNA.
DR   PIR; H64303; H64303.
DR   AlphaFoldDB; Q60341; -.
DR   SMR; Q60341; -.
DR   STRING; 243232.MJ_0032; -.
DR   EnsemblBacteria; AAB98011; AAB98011; MJ_0032.
DR   KEGG; mja:MJ_0032; -.
DR   eggNOG; arCOG02651; Archaea.
DR   HOGENOM; CLU_037958_0_0_2; -.
DR   InParanoid; Q60341; -.
DR   OMA; REYGCEK; -.
DR   PhylomeDB; Q60341; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0050454; F:coenzyme F420 hydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0052592; F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor; IBA:GO_Central.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR017679; FrhB_archaea.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   PANTHER; PTHR31332; PTHR31332; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
DR   TIGRFAMs; TIGR03289; frhB; 1.
PE   3: Inferred from homology;
KW   Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Reference proteome.
FT   CHAIN           1..287
FT                   /note="Coenzyme F420 hydrogenase subunit beta"
FT                   /id="PRO_0000159226"
SQ   SEQUENCE   287 AA;  32054 MW;  7CE7D4E6E970940E CRC64;
     MRWNRMNPFG SYKKVVSARS TLKEVLKKAQ DGGIVSTAFI YGLENNLLDG VIVADNAGEF
     KAVPKVATTP EEVLEAAGTK YTVCPNISVL KSAVREYGCE KIGVVGTPCQ VRAVRKLMKY
     PVGFRHVPDK IALIIGIFCM ENFPYYGLKL IVEEHCGVKM EDVVKMDIGK GKFWVYTRWG
     ETKAIKLKET HPYEQIACHV CTDYTAELAD ISTGSVGSPD GWSTVFIRTA KGEEIFNKMV
     EDGYLEVKPI EEVKPGLGLV EKLALQKKEK NMKEIEHRKE LGLPIPY