ID   FRHB_METVO              Reviewed;         280 AA.
AC   Q00391;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Coenzyme F420 hydrogenase subunit beta;
DE            EC=1.12.98.1;
DE   AltName: Full=8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta;
DE            Short=FRH;
GN   Name=frhB; Synonyms=frcB;
OS   Methanococcus voltae.
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=2188;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33273 / DSM 1537 / NBRC 100457 / OCM 70 / PS;
RX   PubMed=1603063; DOI=10.1007/bf00587582;
RA   Halboth S., Klein A.;
RT   "Methanococcus voltae harbors four gene clusters potentially encoding two
RT   [NiFe] and two [NiFeSe] hydrogenases, each of the cofactor F420-reducing or
RT   F420-non-reducing types.";
RL   Mol. Gen. Genet. 233:217-224(1992).
CC   -!- FUNCTION: Reduces the physiological low-potential two-electron acceptor
CC       coenzyme F420, and the artificial one-electron acceptor methylviologen.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + H2 + oxidized coenzyme F420-(gamma-L-Glu)(n) = reduced
CC         coenzyme F420-(gamma-L-Glu)(n); Xref=Rhea:RHEA:23760, Rhea:RHEA-
CC         COMP:12939, Rhea:RHEA-COMP:14378, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:18276, ChEBI:CHEBI:133980, ChEBI:CHEBI:139511;
CC         EC=1.12.98.1;
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC   -!- COFACTOR:
CC       Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SUBUNIT: Complex of alpha, beta and gamma subunits.
CC   -!- SIMILARITY: Belongs to the FrhB family. {ECO:0000305}.
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DR   EMBL; X61201; CAA43499.1; -; Genomic_DNA.
DR   PIR; S16724; S16724.
DR   AlphaFoldDB; Q00391; -.
DR   SMR; Q00391; -.
DR   GO; GO:0050454; F:coenzyme F420 hydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   InterPro; IPR007516; Co_F420_Hydgase/DH_bsu_N.
DR   InterPro; IPR045220; FRHB/FDHB/HCAR-like.
DR   InterPro; IPR017679; FrhB_archaea.
DR   InterPro; IPR007525; FrhB_FdhB_C.
DR   PANTHER; PTHR31332; PTHR31332; 1.
DR   Pfam; PF04432; FrhB_FdhB_C; 1.
DR   Pfam; PF04422; FrhB_FdhB_N; 1.
DR   TIGRFAMs; TIGR03289; frhB; 1.
PE   3: Inferred from homology;
KW   Iron; Iron-sulfur; Metal-binding; Oxidoreductase.
FT   CHAIN           1..280
FT                   /note="Coenzyme F420 hydrogenase subunit beta"
FT                   /id="PRO_0000159230"
SQ   SEQUENCE   280 AA;  30868 MW;  CA00DC1F6DAE46AA CRC64;
     MPFGNYKTAI SARATDKEIL KKAQDGGYVS AAHIYGLESK LLDGVSVAAD TEDGCNAAPK
     VATTPEEVLN AAGAKYTVSP NVSVLKDAVR EYALEKVGIV GTPCQVQAIR KLIKYPMGFR
     HTDSRIVMGI FCMENFSYEG MKAIVEEYAG IRMDDVLKTD IGKGKFWVYS KYGDVKSVKL
     KDTHMYEQKS CHICTDYTAE LADISTGSVG SPDGWSTIFI RTEKGEAYIN KMVEAGVLET
     KNIDDVKPGL DLVQKLSLQK KEKNEKEIVQ RRELGLPVPY