ID   FRIES_KALDA             Reviewed;         767 AA.
AC   E2IUA8;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Friedelin synthase;
DE            Short=KdFRS;
DE            EC=5.4.99.50;
OS   Kalanchoe daigremontiana (Devil's backbone) (Bryophyllum daigremontianum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Saxifragales; Crassulaceae; Kalanchoe.
OX   NCBI_TaxID=23013;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=20610397; DOI=10.1074/jbc.m109.098871;
RA   Wang Z., Yeats T., Han H., Jetter R.;
RT   "Cloning and characterization of oxidosqualene cyclases from Kalanchoe
RT   daigremontiana: enzymes catalyzing up to 10 rearrangement steps yielding
RT   friedelin and other triterpenoids.";
RL   J. Biol. Chem. 285:29703-29712(2010).
CC   -!- FUNCTION: Oxidosqualene cyclase that generates friedelin, a
CC       triterpenoid product with the maximum number of rearrangements
CC       possible. Friedelin is probably required to coat the leaf exterior as
CC       defense compound against pathogens or herbivores.
CC       {ECO:0000269|PubMed:20610397}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = friedelin; Xref=Rhea:RHEA:31863,
CC         ChEBI:CHEBI:5171, ChEBI:CHEBI:15441; EC=5.4.99.50;
CC         Evidence={ECO:0000269|PubMed:20610397};
CC   -!- TISSUE SPECIFICITY: Expressed only in the epidermal cells on both sides
CC       of the leaf and not in internal leaf tissues.
CC       {ECO:0000269|PubMed:20610397}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; HM623870; ADK35125.1; -; mRNA.
DR   AlphaFoldDB; E2IUA8; -.
DR   SMR; E2IUA8; -.
DR   KEGG; ag:ADK35125; -.
DR   BioCyc; MetaCyc:MON-17975; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016866; F:intramolecular transferase activity; IDA:UniProtKB.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..767
FT                   /note="Friedelin synthase"
FT                   /id="PRO_0000418483"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          640..681
FT                   /note="PFTB 2"
FT   ACT_SITE        485
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   767 AA;  88396 MW;  A9F85D0CFB092B74 CRC64;
     MWKLKIAEGG SDPYIYTTNN FVGRQIWEFD PQATDPQQLA KVEAARLNFY NHRHKIKPSS
     DLLWRLQFLE EKDFRQNIAQ VKVEDGEEVS YEAATAALKR GVHFYSALQA SDGHWPAENA
     GPMFFMSPLV MCLYITGHLN TIFTEEHRRE TLRYIYYHQN EDGGWGFHIE GQSTMFGTVL
     NYICMRLLGE GPEGGQDNAV SRGRKWILDH GGATAIPSWG KTWLSIMGLC DWSGCNPMPP
     EFWLLPSYLP MHPAKMWCYC RMVYMPMSYL YGKRFTTHIT PLILQLREEL HTQPYDQINW
     KKVRHVCCKE DTYYPHPILQ DLIWDTLYLT TEPLLTRWPL NKLIRERALK KTMKHIHYED
     ENSRYIVIGA VEKVLCMLAC WVEDPNGDYF KKHLARVPDY FWVAEDGMKI QSFGSQHWDT
     AFFVQALLAS DMTDEIRTTL AKAHDCIKKS QVKDNPSGDF RSMYRHISKG AWTFSDQDHG
     WQLSDCTAEG LKCCLLFSLM QPEVVGEAMP PERLYDSVNV LLYLQSKNGG MPGWEPAGES
     EWLELLNPTE FFENIVIEHE YVECTSSAVQ ALVLFKKLYP LHRRKEVERF ITNGAKYLED
     IQMPDGSWYG NWGVCFTYGA WFALEGLSAA GKTYNNCAAV RKGVDFLLNI QLEDGGWGES
     YQSCPDKKYV PLEDNRSNLV QTSWALMGLI YAGQADRDPT PLHRAAKLLI NSQLEDGDFP
     QQEIAGVFKM NCTLHFAAYR NIFPIWALAV YRRFCNPNSE AISKPSK