FRLA_ECOLI
ID FRLA_ECOLI Reviewed; 445 AA.
AC P45539; P76686; Q2M736;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Probable fructoselysine/psicoselysine transporter FrlA;
GN Name=frlA {ECO:0000303|PubMed:12147680}; Synonyms=yhfM;
GN OrderedLocusNames=b3370, JW3333;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION, PATHWAY, AND INDUCTION.
RX PubMed=12147680; DOI=10.1074/jbc.m200863200;
RA Wiame E., Delpierre G., Collard F., Van Schaftingen E.;
RT "Identification of a pathway for the utilization of the Amadori product
RT fructoselysine in Escherichia coli.";
RL J. Biol. Chem. 277:42523-42529(2002).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX PubMed=14641112; DOI=10.1042/bj20031527;
RA Wiame E., Van Schaftingen E.;
RT "Fructoselysine 3-epimerase, an enzyme involved in the metabolism of the
RT unusual Amadori compound psicoselysine in Escherichia coli.";
RL Biochem. J. 378:1047-1052(2004).
CC -!- FUNCTION: Is likely involved in the transport of fructoselysine and
CC psicoselysine to the cytoplasm, where they are degraded.
CC {ECO:0000305|PubMed:12147680, ECO:0000305|PubMed:14641112}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(6)-(D-fructosyl)-L-lysine(in) = N(6)-(D-fructosyl)-L-
CC lysine(out); Xref=Rhea:RHEA:28454, ChEBI:CHEBI:61393;
CC Evidence={ECO:0000305|PubMed:12147680, ECO:0000305|PubMed:14641112};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28456;
CC Evidence={ECO:0000305|PubMed:12147680, ECO:0000305|PubMed:14641112};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N(6)-(D-psicosyl)-L-lysine(in) = N(6)-(D-psicosyl)-L-
CC lysine(out); Xref=Rhea:RHEA:28450, ChEBI:CHEBI:61403;
CC Evidence={ECO:0000305|PubMed:14641112};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28452;
CC Evidence={ECO:0000305|PubMed:14641112};
CC -!- PATHWAY: Carbohydrate metabolism; fructoselysine degradation.
CC {ECO:0000269|PubMed:14641112, ECO:0000305|PubMed:12147680}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: Induced by fructoselysine. Makes part of the frl operon with
CC FrlB, FrlC, FrlD and FrlR. {ECO:0000305|PubMed:12147680}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are unable to grow on
CC fructoselysine or psicoselysine, where they do grow on glucose.
CC {ECO:0000269|PubMed:14641112}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. {ECO:0000305}.
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DR EMBL; U18997; AAA58167.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U00096; AAC76395.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77920.1; -; Genomic_DNA.
DR PIR; E65131; E65131.
DR RefSeq; NP_417829.2; NC_000913.3.
DR AlphaFoldDB; P45539; -.
DR SMR; P45539; -.
DR BioGRID; 4262479; 5.
DR DIP; DIP-12322N; -.
DR IntAct; P45539; 1.
DR STRING; 511145.b3370; -.
DR TCDB; 2.A.3.8.17; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; P45539; -.
DR PRIDE; P45539; -.
DR EnsemblBacteria; AAC76395; AAC76395; b3370.
DR EnsemblBacteria; BAE77920; BAE77920; BAE77920.
DR GeneID; 947878; -.
DR KEGG; ecj:JW3333; -.
DR KEGG; eco:b3370; -.
DR PATRIC; fig|1411691.4.peg.3359; -.
DR EchoBASE; EB2745; -.
DR eggNOG; COG0531; Bacteria.
DR HOGENOM; CLU_007946_3_4_6; -.
DR InParanoid; P45539; -.
DR OMA; HKAFGIH; -.
DR PhylomeDB; P45539; -.
DR BioCyc; EcoCyc:YHFM-MON; -.
DR BioCyc; MetaCyc:YHFM-MON; -.
DR UniPathway; UPA00784; -.
DR PRO; PR:P45539; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1901281; P:fructoselysine catabolic process; IMP:EcoCyc.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..445
FT /note="Probable fructoselysine/psicoselysine transporter
FT FrlA"
FT /id="PRO_0000054247"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..435
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 445 AA; 47577 MW; B98DF5944A5E1FEF CRC64;
MGSQELQRKL GFWAVLAIAV GTTVGSGIFV SVGEVAKAAG TPWLTVLAFV IGGLIVIPQM
CVYAELSTAY PENGADYVYL KNAGSRPLAF LSGWASFWAN DAPSLSIMAL AIVSNLGFLT
PIDPLLGKFI AAGLIIAFML LHLRSVEGGA AFQTLITIAK IIPFTIVIGL GIFWFKAENF
AAPTTTAIGA TGSFMALLAG ISATSWSYTG MASICYMTGE IKNPGKTMPR ALIGSCLLVL
VLYTLLALVI SGLMPFDKLA NSETPISDAL TWIPALGSTA GIFVAITAMI VILGSLSSCV
MYQPRLEYAM AKDNLFFKCF GHVHPKYNTP DVSIILQGAL GIFFIFVSDL TSLLGYFTLV
MCFKNTLTFG SIIWCRKRDD YKPLWRTPAF GLMTTLAIAS SLILVASTFV WAPIPGLICA
VIVIATGLPA YAFWAKRSRQ LNALS
