ID   FRLD_ECO57              Reviewed;         261 AA.
AC   Q8X839; Q8X3V9;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   24-OCT-2003, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Fructoselysine 6-kinase {ECO:0000250|UniProtKB:P45543};
DE            EC=2.7.1.218 {ECO:0000250|UniProtKB:P45543};
GN   Name=frlD; OrderedLocusNames=Z4734/Z4735, ECs4224;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Catalyzes the ATP-dependent phosphorylation of fructoselysine
CC       to fructoselysine 6-phosphate. Functions in a fructoselysine
CC       degradation pathway that allows E.coli to grow on fructoselysine or
CC       psicoselysine. To a much lesser extenst, is also able to phosphorylate
CC       psicoselysine. {ECO:0000250|UniProtKB:P45543}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + N(6)-(D-fructosyl)-L-lysine = ADP + H(+) + N(6)-(6-
CC         phospho-D-fructosyl)-L-lysine; Xref=Rhea:RHEA:28378,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:61392,
CC         ChEBI:CHEBI:61393, ChEBI:CHEBI:456216; EC=2.7.1.218;
CC         Evidence={ECO:0000250|UniProtKB:P45543};
CC   -!- PATHWAY: Carbohydrate metabolism; fructoselysine degradation; D-glucose
CC       6-phosphate and lysine from fructoselysine: step 1/2.
CC       {ECO:0000250|UniProtKB:P45543}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P45543}.
CC   -!- SIMILARITY: Belongs to the carbohydrate kinase PfkB family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG58481.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAG58482.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAB37647.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG58481.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AE005174; AAG58482.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BA000007; BAB37647.1; ALT_SEQ; Genomic_DNA.
DR   PIR; E86002; E86002.
DR   PIR; F86002; F86002.
DR   PIR; H91156; H91156.
DR   RefSeq; NP_312251.1; NC_002695.1.
DR   AlphaFoldDB; Q8X839; -.
DR   SMR; Q8X839; -.
DR   STRING; 155864.EDL933_4580; -.
DR   EnsemblBacteria; AAG58481; AAG58481; Z4734.
DR   EnsemblBacteria; AAG58482; AAG58482; Z4735.
DR   KEGG; ece:Z4734; -.
DR   KEGG; ece:Z4735; -.
DR   PATRIC; fig|386585.9.peg.4409; -.
DR   eggNOG; COG0524; Bacteria.
DR   HOGENOM; CLU_2167135_0_0_6; -.
DR   UniPathway; UPA00784; UER00769.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016773; F:phosphotransferase activity, alcohol group as acceptor; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1190.20; -; 1.
DR   InterPro; IPR002173; Carboh/pur_kinase_PfkB_CS.
DR   InterPro; IPR011611; PfkB_dom.
DR   InterPro; IPR029056; Ribokinase-like.
DR   Pfam; PF00294; PfkB; 1.
DR   SUPFAM; SSF53613; SSF53613; 1.
DR   PROSITE; PS00583; PFKB_KINASES_1; 1.
DR   PROSITE; PS00584; PFKB_KINASES_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Transferase.
FT   CHAIN           1..261
FT                   /note="Fructoselysine 6-kinase"
FT                   /id="PRO_0000080065"
SQ   SEQUENCE   261 AA;  28260 MW;  8197354632F671E5 CRC64;
     MKTLATIGDN CVDIYPQLNK AFSGGNAVNV AVYCTRYGIK PGCITWVGDD DYGTKLKQDL
     ASMGVDISHV HTKHGVTAQT QVELHDNDRV FGDYTEGVMA DFALSEEDYA WLAQYDIVHA
     AIWGHAEDAF PQLHAAGKLT AFDVSDKWDS PLWQTLVPHL DFAFASAPQE DEALRLKMKA
     IVARGAGVVI VTLGENGSIA WDGVQFWRQA PEQVTVIDTM GAGDSFIAGF LCGWSAGMTL
     PQAMAQGTAC AAQTIQYHGA W