FRLR_ECOLI
ID FRLR_ECOLI Reviewed; 243 AA.
AC P45544; Q2M740;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Probable fructoselysine utilization operon transcriptional repressor {ECO:0000303|PubMed:12147680};
DE AltName: Full=HTH-type transcriptional regulator FrlR {ECO:0000305};
GN Name=frlR {ECO:0000303|PubMed:12147680}; Synonyms=yhfR;
GN OrderedLocusNames=b3375, JW5698;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION, PATHWAY, AND INDUCTION.
RX PubMed=12147680; DOI=10.1074/jbc.m200863200;
RA Wiame E., Delpierre G., Collard F., Van Schaftingen E.;
RT "Identification of a pathway for the utilization of the Amadori product
RT fructoselysine in Escherichia coli.";
RL J. Biol. Chem. 277:42523-42529(2002).
CC -!- FUNCTION: May regulate the transcription of the frlABCDR operon,
CC involved in the utilization of fructoselysine and psicoselysine.
CC {ECO:0000305|PubMed:12147680}.
CC -!- PATHWAY: Carbohydrate metabolism; fructoselysine degradation
CC [regulation]. {ECO:0000305|PubMed:12147680}.
CC -!- INTERACTION:
CC P45544; P14294: topB; NbExp=2; IntAct=EBI-562481, EBI-552080;
CC -!- INDUCTION: Induced by fructoselysine. Makes part of the frl operon with
CC FrlA, FrlB, FrlC and FrlD. {ECO:0000305|PubMed:12147680}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA58172.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U18997; AAA58172.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76400.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77916.1; -; Genomic_DNA.
DR PIR; B65132; B65132.
DR RefSeq; NP_417834.6; NC_000913.3.
DR RefSeq; WP_001276847.1; NZ_STEB01000004.1.
DR AlphaFoldDB; P45544; -.
DR SMR; P45544; -.
DR BioGRID; 4259581; 14.
DR BioGRID; 852194; 4.
DR DIP; DIP-12327N; -.
DR IntAct; P45544; 6.
DR STRING; 511145.b3375; -.
DR PaxDb; P45544; -.
DR PRIDE; P45544; -.
DR EnsemblBacteria; AAC76400; AAC76400; b3375.
DR EnsemblBacteria; BAE77916; BAE77916; BAE77916.
DR GeneID; 66672745; -.
DR GeneID; 947885; -.
DR KEGG; ecj:JW5698; -.
DR KEGG; eco:b3375; -.
DR PATRIC; fig|1411691.4.peg.3355; -.
DR EchoBASE; EB2749; -.
DR eggNOG; COG2188; Bacteria.
DR HOGENOM; CLU_063236_3_0_6; -.
DR InParanoid; P45544; -.
DR OMA; RYKLWVP; -.
DR PhylomeDB; P45544; -.
DR BioCyc; EcoCyc:G7727-MON; -.
DR UniPathway; UPA00784; -.
DR PRO; PR:P45544; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd07377; WHTH_GntR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.1410.10; -; 1.
DR InterPro; IPR028978; Chorismate_lyase_/UTRA_dom_sf.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR011663; UTRA.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00392; GntR; 1.
DR Pfam; PF07702; UTRA; 1.
DR PRINTS; PR00035; HTHGNTR.
DR SMART; SM00345; HTH_GNTR; 1.
DR SMART; SM00866; UTRA; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF64288; SSF64288; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..243
FT /note="Probable fructoselysine utilization operon
FT transcriptional repressor"
FT /id="PRO_0000050642"
FT DOMAIN 10..78
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 38..57
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
SQ SEQUENCE 243 AA; 27821 MW; 973FEE8232B9B0F6 CRC64;
MSATDRYSHQ LLYATVRQRL LDDIAQGVYQ AGQQIPTENE LCTQYNVSRI TIRKAISDLV
ADGVLIRWQG KGTFVQSQKV ENALLTVSGF TDFGVSQGKA TKEKVIEQER VSAAPFCEKL
NIPGNSEVFH LCRVMYLDKE PLFIDSSWIP LSRYPDFDEI YVEGSSTYQL FQERFDTRVV
SDKKTIDIFA ATRPQAKWLK CELGEPLFRI SKIAFDQNDK PVHVSELFCR ANRITLTIDN
KRH
