ID   ALDH_GEOSE              Reviewed;         488 AA.
AC   P42329;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Aldehyde dehydrogenase, thermostable;
DE            EC=1.2.1.5;
GN   Name=aldHT;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SIC1;
RA   Imanaka T., Ohta T., Sakoda H., Widhyastuti N., Matsuoka M.;
RT   "Cloning, nucleotide sequence, and efficient expression of the gene coding
RT   for thermostable aldehyde dehydrogenase from Bacillus stearothermophilus,
RT   and characterization of the enzyme.";
RL   J. Ferment. Bioeng. 76:161-167(1993).
CC   -!- FUNCTION: Oxidizes several aliphatic aldehydes, particularly c6-
CC       aliphatic aldehyde and hexanal, but does not oxidize benzaldehyde.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + NADP(+) = a carboxylate + 2 H(+) + NADPH;
CC         Xref=Rhea:RHEA:11888, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.2.1.5;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.5;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Optimum temperature is 55-60 degrees Celsius.;
CC   -!- PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol:
CC       step 2/2.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; D13846; BAA02975.1; -; Genomic_DNA.
DR   PIR; I39769; I39769.
DR   AlphaFoldDB; P42329; -.
DR   SMR; P42329; -.
DR   UniPathway; UPA00780; UER00768.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IEA:RHEA.
DR   GO; GO:0033721; F:aldehyde dehydrogenase (NADP+) activity; IEA:RHEA.
DR   GO; GO:0006068; P:ethanol catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..488
FT                   /note="Aldehyde dehydrogenase, thermostable"
FT                   /id="PRO_0000056442"
FT   ACT_SITE        255
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        289
FT                   /evidence="ECO:0000250"
FT   BINDING         233..238
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   488 AA;  52915 MW;  29E824451985D9ED CRC64;
     MKVQTEIKTY FNYINGNWVS SVSNNVEPSI NPANRHDIVG YVQRSTLEDV NEAVTAANEA
     QTSWWKRSGV ERGEYLYKAA HILEQCLQDI AETMTREMGK TLAEAKAETM RGVHILRYYA
     GEGARKIGDV IPSSDSEGLL FTTRVPLGVV GVISPWNFPV AIPIWKMAPA LVYGNTVVLK
     PASETAVTAA KVIECFHEAG FPKGVVNMVC GSGSVVGQGI ANHPDIDGVT FTGSNTVGKQ
     VGRAAFERGA KYQLEMGGKN PVIVAKDADL DLAVEGTISG GLRSTGQKCT ATSRVFIERE
     VYEPFKAKLL ERVKQLKIGN GLDAETWMGP CASESQFHTV LSYIEKGKSE GAKLIYGGNR
     CLEGELANGF FVEPTIFEDV DLQMTIAREE IFGPVLALIQ VDSIEEAIKL ANDTEYGLSA
     SIYTKNIGNA LEFIKDIEAG LIKVNAETAG VEFQAPFGGM KQSSSHSREQ GQAAIEFFTS
     IKTVFVKA