FRUR_VIBCH
ID FRUR_VIBCH Reviewed; 326 AA.
AC Q9KM69;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Fructose operon regulatory protein {ECO:0000305};
DE AltName: Full=Catabolite repressor/activator {ECO:0000303|PubMed:33649152};
GN Name=fruR {ECO:0000303|PubMed:33476373};
GN Synonyms=cra {ECO:0000303|PubMed:33649152};
GN OrderedLocusNames=VC_A0519 {ECO:0000312|EMBL:AAF96422.1};
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
RN [2]
RP FUNCTION, DNA-BINDING, ACTIVITY REGULATION, SUBUNIT, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=33476373; DOI=10.1093/nar/gkab013;
RA Yoon C.K., Kang D., Kim M.K., Seok Y.J.;
RT "Vibrio cholerae FruR facilitates binding of RNA polymerase to the fru
RT promoter in the presence of fructose 1-phosphate.";
RL Nucleic Acids Res. 49:1397-1410(2021).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=33649152; DOI=10.1128/jb.00044-21;
RA Beck C., Perry S., Stoebel D.M., Liu J.M.;
RT "Cra and cAMP receptor protein have opposing roles in the regulation of
RT fruB in Vibrio cholerae.";
RL J. Bacteriol. 203:0-0(2021).
CC -!- FUNCTION: Regulates the expression of the fruBKA (fru) operon, which
CC encodes proteins involved in the import and metabolism of fructose
CC (PubMed:33476373, PubMed:33649152). In the absence of fructose 1-
CC phosphate (F1P), binds to the promoter region of fruB, interferes with
CC the binding of the RNA polymerase (RNAP) to the promoter and represses
CC the expression of the operon (PubMed:33476373, PubMed:33649152). In the
CC presence of F1P, activates the transcription of the fru operon by
CC facilitating the binding of RNAP to the promoter (PubMed:33476373).
CC Essential for the expression of the fru operon and thus for growth on
CC fructose (PubMed:33476373). {ECO:0000269|PubMed:33476373,
CC ECO:0000269|PubMed:33649152}.
CC -!- ACTIVITY REGULATION: Interaction with F1P may induce a structural
CC change in the DNA spacer region between the -35 and -10 elements,
CC thereby facilitating RNAP binding to the promoter to trigger the
CC transcriptional activation of the fru operon. Interaction with F1P does
CC not release FruR from its binding sequence.
CC {ECO:0000269|PubMed:33476373}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:33476373}.
CC -!- INDUCTION: Expressed at the highest levels in fructose media
CC (PubMed:33476373, PubMed:33649152). Expression can be modulated by CRP
CC in the presence of fructose and glucose (PubMed:33649152).
CC {ECO:0000269|PubMed:33476373, ECO:0000269|PubMed:33649152}.
CC -!- DISRUPTION PHENOTYPE: Does not affect growth on glucose. Mutant shows a
CC severe growth defect on fructose (PubMed:33476373). In the absence of
CC the gene, expression of the fru genes increases in glucose medium
CC (PubMed:33649152). {ECO:0000269|PubMed:33476373,
CC ECO:0000269|PubMed:33649152}.
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DR EMBL; AE003853; AAF96422.1; -; Genomic_DNA.
DR PIR; E82450; E82450.
DR RefSeq; NP_232910.1; NC_002506.1.
DR RefSeq; WP_000171125.1; NZ_LT906615.1.
DR SMR; Q9KM69; -.
DR STRING; 243277.VC_A0519; -.
DR PRIDE; Q9KM69; -.
DR DNASU; 2612773; -.
DR EnsemblBacteria; AAF96422; AAF96422; VC_A0519.
DR GeneID; 57741923; -.
DR KEGG; vch:VC_A0519; -.
DR PATRIC; fig|243277.26.peg.3145; -.
DR eggNOG; COG1609; Bacteria.
DR HOGENOM; CLU_037628_6_0_6; -.
DR OMA; NSRKAGY; -.
DR BioCyc; VCHO:VCA0519-MON; -.
DR Proteomes; UP000000584; Chromosome 2.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009750; P:response to fructose; IEA:InterPro.
DR CDD; cd01392; HTH_LacI; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR012781; Fruct_sucro_rep.
DR InterPro; IPR000843; HTH_LacI.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR001761; Peripla_BP/Lac1_sug-bd_dom.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF00356; LacI; 1.
DR Pfam; PF00532; Peripla_BP_1; 1.
DR SMART; SM00354; HTH_LACI; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR TIGRFAMs; TIGR02417; fruct_sucro_rep; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
DR PROSITE; PS50932; HTH_LACI_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..326
FT /note="Fructose operon regulatory protein"
FT /id="PRO_0000453369"
FT DOMAIN 1..58
FT /note="HTH lacI-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT DNA_BIND 3..22
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
SQ SEQUENCE 326 AA; 35995 MW; A9A62681CFE9EB5A CRC64;
MTLDEIAKLA GVSKTTASYV INGKAQKYRI SEKTQHKVMA VVEQYNFRPD HAASALRAGN
SRSFGLIIPD LENTSYARLA KLLEQNSRQA GYQILIACSD DDPQIEMAAA EALVSRRIDA
LFVASGIPSA SEYYLKLQQS GTPVIAIDRA LDDEYFSCVI SEDFGAAFEL TRSVLTQDVH
SVGLVGALPE LNVSREREQG FAMAVKQRGL PTTLGYGEHF NREEGRKVFA KWVANDQLPD
AVVATSYTLL EGILDVLLEQ PELMQKVRLA TFGDNRLLDF LPIRVNSLPQ QFELIADSAL
ALALNASAKR YQTGIELIPR QLKVRT
