ID   ALDH_PSEOL              Reviewed;         483 AA.
AC   P12693;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Aldehyde dehydrogenase;
DE            EC=1.2.1.3;
GN   Name=alkH;
OS   Pseudomonas oleovorans.
OG   Plasmid OCT.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas;
OC   Pseudomonas oleovorans/pseudoalcaligenes group.
OX   NCBI_TaxID=301;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=GPo1;
RX   PubMed=2647719; DOI=10.1016/s0021-9258(18)83565-7;
RA   Kok M., Oldenhuis R., van der Linden M.P.G., Meulenberg C.H.C., Kingma J.,
RA   Witholt B.;
RT   "The Pseudomonas oleovorans alkBAC operon encodes two structurally related
RT   rubredoxins and an aldehyde dehydrogenase.";
RL   J. Biol. Chem. 264:5442-5451(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=GPo1;
RX   PubMed=1453953; DOI=10.1111/j.1365-2958.1992.tb01769.x;
RA   van Beilen J.B., Eggink G., Enequist H., Bos R., Witholt B.;
RT   "DNA sequence determination and functional characterization of the OCT-
RT   plasmid-encoded alkJKL genes of Pseudomonas oleovorans.";
RL   Mol. Microbiol. 6:3121-3136(1992).
RN   [3]
RP   INDUCTION.
RX   PubMed=10692156; DOI=10.1046/j.1365-2958.2000.01751.x;
RA   Canosa I., Sanchez-Romero J.M., Yuste L., Rojo F.;
RT   "A positive feedback mechanism controls expression of AlkS, the
RT   transcriptional regulator of the Pseudomonas oleovorans alkane degradation
RT   pathway.";
RL   Mol. Microbiol. 35:791-799(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.3;
CC   -!- PATHWAY: Hydrocarbon metabolism; alkane degradation.
CC   -!- INDUCTION: Induced by AlkS. {ECO:0000269|PubMed:10692156}.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; AJ245436; CAB54053.1; -; Genomic_DNA.
DR   PIR; C32850; D31266.
DR   AlphaFoldDB; P12693; -.
DR   SMR; P12693; -.
DR   PRIDE; P12693; -.
DR   BioCyc; MetaCyc:MON-1082; -.
DR   UniPathway; UPA00191; -.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0043878; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0043448; P:alkane catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006081; P:cellular aldehyde metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   InterPro; IPR012394; Aldehyde_DH_NAD(P).
DR   PANTHER; PTHR43570; PTHR43570; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PIRSF; PIRSF036492; ALDH; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   2: Evidence at transcript level;
KW   NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..483
FT                   /note="Aldehyde dehydrogenase"
FT                   /id="PRO_0000056451"
FT   ACT_SITE        226
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        260
FT                   /evidence="ECO:0000250"
FT   BINDING         204..209
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   483 AA;  52766 MW;  E65E9D00A4E8F336 CRC64;
     MTIPISLAKL NSSADTHSAL EVFNLQKVAS SARRGKFGIA ERIAALNLLK ETIQRREPEI
     IAALAADFRK PASEVKLTEI FPVLQEINHA KRNLKDWMKP RRVRAALSVA GTRAGLRYEP
     KGVCLIIAPW NYPFNLSFGP LVSALAAGNS VVIKPSELTP HTATLIGSIV REAFSVDLVA
     VVEGDAAVSQ ELLALPFDHI FFTGSPRVGK LVMEAASKTL ASVTLELGGK SPTIIGPTAN
     LPKAARNIVW GKFSNNGQTC IAPDHVFVHR CIAQKFNEIL VKEIVRVYGK DFAAQRRSAD
     YCRIVNDQHF NRINKLLTDA KAKGAKILQG GQVDATERLV VPTVLSNVTA AMDINHEEIF
     GPLLPIIEYD DIDSVIKRVN DGDKPLALYV FSEDKQFVNN IVARTSSGSV GVNLSVVHFL
     HPNLPFGGVN NSGIGSAHGV YGFRAFSHEK PVLIDKFSIT HWLFPPYTKK VKQLIGITVK
     YLS