FSCN3_HUMAN
ID FSCN3_HUMAN Reviewed; 498 AA.
AC Q9NQT6; A4D0Z2; A6NLL7; B2RA62; B4DU68;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Fascin-3;
DE AltName: Full=Testis fascin;
GN Name=FSCN3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11925108; DOI=10.1006/excr.2002.5486;
RA Tubb B., Mulholland D.J., Vogl W., Lan Z.J., Niederberger C., Cooney A.,
RA Bryan J.;
RT "Testis fascin (FSCN3): a novel paralog of the actin-bundling protein
RT fascin expressed specifically in the elongate spermatid head.";
RL Exp. Cell Res. 275:92-109(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Prostate, and Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Acts as an actin bundling protein. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9NQT6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9NQT6-2; Sequence=VSP_057001, VSP_057002, VSP_057003;
CC -!- TISSUE SPECIFICITY: Expressed in testis.
CC -!- SIMILARITY: Belongs to the fascin family. {ECO:0000305}.
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DR EMBL; AF281049; AAF91439.1; -; mRNA.
DR EMBL; AK300518; BAG62230.1; -; mRNA.
DR EMBL; AK314050; BAG36759.1; -; mRNA.
DR EMBL; AC000357; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC073934; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH236947; EAL24319.1; -; Genomic_DNA.
DR EMBL; CH471070; EAW83632.1; -; Genomic_DNA.
DR EMBL; BC035606; AAH35606.1; -; mRNA.
DR CCDS; CCDS34746.1; -. [Q9NQT6-1]
DR RefSeq; NP_065102.1; NM_020369.2. [Q9NQT6-1]
DR AlphaFoldDB; Q9NQT6; -.
DR SMR; Q9NQT6; -.
DR BioGRID; 119023; 17.
DR IntAct; Q9NQT6; 3.
DR STRING; 9606.ENSP00000265825; -.
DR iPTMnet; Q9NQT6; -.
DR PhosphoSitePlus; Q9NQT6; -.
DR BioMuta; FSCN3; -.
DR DMDM; 18203315; -.
DR MassIVE; Q9NQT6; -.
DR PaxDb; Q9NQT6; -.
DR PeptideAtlas; Q9NQT6; -.
DR PRIDE; Q9NQT6; -.
DR ProteomicsDB; 5155; -.
DR ProteomicsDB; 82186; -. [Q9NQT6-1]
DR Antibodypedia; 45993; 157 antibodies from 22 providers.
DR DNASU; 29999; -.
DR Ensembl; ENST00000265825.6; ENSP00000265825.5; ENSG00000106328.10. [Q9NQT6-1]
DR GeneID; 29999; -.
DR KEGG; hsa:29999; -.
DR MANE-Select; ENST00000265825.6; ENSP00000265825.5; NM_020369.3; NP_065102.1.
DR UCSC; uc003vmd.3; human. [Q9NQT6-1]
DR CTD; 29999; -.
DR DisGeNET; 29999; -.
DR GeneCards; FSCN3; -.
DR HGNC; HGNC:3961; FSCN3.
DR HPA; ENSG00000106328; Tissue enriched (testis).
DR MIM; 615800; gene.
DR neXtProt; NX_Q9NQT6; -.
DR OpenTargets; ENSG00000106328; -.
DR PharmGKB; PA28379; -.
DR VEuPathDB; HostDB:ENSG00000106328; -.
DR eggNOG; ENOG502QSD9; Eukaryota.
DR GeneTree; ENSGT00950000183157; -.
DR HOGENOM; CLU_030960_2_0_1; -.
DR InParanoid; Q9NQT6; -.
DR OMA; QPDCIQL; -.
DR PhylomeDB; Q9NQT6; -.
DR TreeFam; TF323992; -.
DR PathwayCommons; Q9NQT6; -.
DR SignaLink; Q9NQT6; -.
DR BioGRID-ORCS; 29999; 6 hits in 1067 CRISPR screens.
DR GeneWiki; FSCN3; -.
DR GenomeRNAi; 29999; -.
DR Pharos; Q9NQT6; Tdark.
DR PRO; PR:Q9NQT6; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9NQT6; protein.
DR Bgee; ENSG00000106328; Expressed in sperm and 110 other tissues.
DR ExpressionAtlas; Q9NQT6; baseline and differential.
DR Genevisible; Q9NQT6; HS.
DR GO; GO:0015629; C:actin cytoskeleton; ISS:UniProtKB.
DR GO; GO:0031253; C:cell projection membrane; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; TAS:ProtInc.
DR GO; GO:0030175; C:filopodium; IBA:GO_Central.
DR GO; GO:0030426; C:growth cone; IBA:GO_Central.
DR GO; GO:0030027; C:lamellipodium; IBA:GO_Central.
DR GO; GO:0005902; C:microvillus; IBA:GO_Central.
DR GO; GO:0001726; C:ruffle; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IEA:InterPro.
DR GO; GO:0051017; P:actin filament bundle assembly; IBA:GO_Central.
DR GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR GO; GO:0007163; P:establishment or maintenance of cell polarity; IBA:GO_Central.
DR GO; GO:0007286; P:spermatid development; IEA:InterPro.
DR CDD; cd00257; Fascin; 3.
DR InterPro; IPR008999; Actin-crosslinking.
DR InterPro; IPR010431; Fascin.
DR InterPro; IPR022768; Fascin-domain.
DR InterPro; IPR024703; Fascin_metazoans.
DR InterPro; IPR030143; FSCN3.
DR PANTHER; PTHR10551; PTHR10551; 1.
DR PANTHER; PTHR10551:SF1; PTHR10551:SF1; 1.
DR Pfam; PF06268; Fascin; 2.
DR PIRSF; PIRSF005682; Fascin; 1.
DR SUPFAM; SSF50405; SSF50405; 4.
PE 2: Evidence at transcript level;
KW Actin-binding; Alternative splicing; Cytoplasm; Cytoskeleton;
KW Reference proteome.
FT CHAIN 1..498
FT /note="Fascin-3"
FT /id="PRO_0000219383"
FT VAR_SEQ 1..134
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_057001"
FT VAR_SEQ 431..485
FT /note="AQGGSFWSITSFGTFRPWGKFALNFCIELQGSNLLTVLAPNGFYMRADQSGT
FT LLA -> GGILLVNNILWHLSPLGQVCPQLLYRASGEQLTHCTGPQWLLHASRPKWHPV
FT GRQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_057002"
FT VAR_SEQ 486..498
FT /note="Missing (in isoform 2)"
FT /id="VSP_057003"
FT VARIANT 24
FT /note="A -> S (in dbSNP:rs3779536)"
FT /id="VAR_022021"
FT VARIANT 428
FT /note="H -> L (in dbSNP:rs34394613)"
FT /id="VAR_033939"
SQ SEQUENCE 498 AA; 56624 MW; FF9A1BE44F7D4354 CRC64;
MDETEWIHRH PKAEDLRVGL ISWAGTYLTF EACKNTVTAT AKSLGRRQTW EILVSNEHET
QAVVRLKSVQ GLYLLCECDG TVCYGRPRTS HHGCFLLRFH RNSKWTLQCL ISGRYLESNG
KDVFCTSHVL SAYHMWTPRP ALHVHVILYS PIHRCYARAD PTMGRIWVDA AVPCLEECGF
LLHFRDGCYH LETSTHHFLS HVDRLFSQPS SQTAFHMQVR PGGLVALCDG EGGMLYPQGT
HLLLGMGCNP MRGEEWFILQ HCPTWVSLRS KTGRFISVIY DGEVRAASER LNRMSLFQFE
CDSESPTVQL RSANGYYLSQ RRHRAVMADG HPLESDTFFR MHWNCGRIIL QSCRGRFLGI
APNSLLMANV ILPGPNEEFG ILFANRSFLV LRGRYGYVGS SSGHDLIQCN QDQPDRIHLL
PCRPGIYHFQ AQGGSFWSIT SFGTFRPWGK FALNFCIELQ GSNLLTVLAP NGFYMRADQS
GTLLADSEDI TRECIWEF
