ALDH_STAAM
ID ALDH_STAAM Reviewed; 475 AA.
AC Q99SD6;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Putative aldehyde dehydrogenase {ECO:0000305};
DE EC=1.2.1.3 {ECO:0000305};
GN OrderedLocusNames=SAV2122;
OS Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158878;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu50 / ATCC 700699;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS), AND SUBUNIT.
RA Kim Y., Joachimiak G., Jedrzejczak R., Rubin E., Ioerger T.,
RA Sacchettini J., Joachimiak A.;
RT "Crystal structure of aldehyde dehydrogenase family protein from
RT Staphylococcus aureus.";
RL Submitted (SEP-2011) to the PDB data bank.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH;
CC Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.2.1.3; Evidence={ECO:0000305};
CC -!- SUBUNIT: Homodimer. {ECO:0000305|Ref.2}.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; BA000017; BAB58284.1; -; Genomic_DNA.
DR RefSeq; WP_001206093.1; NC_002758.2.
DR PDB; 3TY7; X-ray; 2.40 A; A/B=1-475.
DR PDBsum; 3TY7; -.
DR AlphaFoldDB; Q99SD6; -.
DR SMR; Q99SD6; -.
DR World-2DPAGE; 0002:Q99SD6; -.
DR PaxDb; Q99SD6; -.
DR EnsemblBacteria; BAB58284; BAB58284; SAV2122.
DR KEGG; sav:SAV2122; -.
DR HOGENOM; CLU_005391_0_2_9; -.
DR OMA; RRMDTGQ; -.
DR PhylomeDB; Q99SD6; -.
DR BioCyc; SAUR158878:SAV_RS11605-MON; -.
DR EvolutionaryTrace; Q99SD6; -.
DR Proteomes; UP000002481; Chromosome.
DR GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0043878; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity; IEA:UniProtKB-EC.
DR GO; GO:0006081; P:cellular aldehyde metabolic process; IEA:InterPro.
DR Gene3D; 3.40.309.10; -; 1.
DR Gene3D; 3.40.605.10; -; 1.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR016160; Ald_DH_CS_CYS.
DR InterPro; IPR029510; Ald_DH_CS_GLU.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR InterPro; IPR012394; Aldehyde_DH_NAD(P).
DR Pfam; PF00171; Aldedh; 1.
DR PIRSF; PIRSF036492; ALDH; 1.
DR SUPFAM; SSF53720; SSF53720; 1.
DR PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 1: Evidence at protein level;
KW 3D-structure; NAD; Oxidoreductase.
FT CHAIN 1..475
FT /note="Putative aldehyde dehydrogenase"
FT /id="PRO_0000293556"
FT ACT_SITE 245
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT ACT_SITE 279
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT BINDING 146..147
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT BINDING 223..224
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT BINDING 246
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT BINDING 379
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250|UniProtKB:P25526"
FT STRAND 6..9
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 12..15
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 17..19
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 21..25
FT /evidence="ECO:0007829|PDB:3TY7"
FT TURN 27..29
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 32..37
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 41..59
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 63..79
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 81..92
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 96..101
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 103..120
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 123..127
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 140..143
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 146..148
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 151..163
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 167..170
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 177..189
FT /evidence="ECO:0007829|PDB:3TY7"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 196..198
FT /evidence="ECO:0007829|PDB:3TY7"
FT TURN 203..206
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 207..212
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 218..221
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 225..228
FT /evidence="ECO:0007829|PDB:3TY7"
FT TURN 235..239
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 241..243
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 251..254
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 260..272
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 273..276
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 284..288
FT /evidence="ECO:0007829|PDB:3TY7"
FT TURN 289..291
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 292..304
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 324..340
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 343..346
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 364..368
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 374..377
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 382..392
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 393..400
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 407..412
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 416..425
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 428..433
FT /evidence="ECO:0007829|PDB:3TY7"
FT HELIX 462..464
FT /evidence="ECO:0007829|PDB:3TY7"
FT STRAND 465..471
FT /evidence="ECO:0007829|PDB:3TY7"
SQ SEQUENCE 475 AA; 51969 MW; 03C66ED0B87BC09A CRC64;
MRDYTKQYIN GEWVESNSNE TIEVINPATE EVIGKVAKGN KADVDKAVEA ADDVYLEFRH
TSVKERQALL DKIVKEYENR KDDIVQAITD ELGAPLSLSE RVHYQMGLNH FVAARDALDN
YEFEERRGDD LVVKEAIGVS GLITPWNFPT NQTSLKLAAA FAAGSPVVLK PSEETPFAAV
ILAEIFDKVG VPKGVFNLVN GDGAGVGNPL SEHPKVRMMS FTGSGPTGSK IMEKAAKDFK
KVSLELGGKS PYIVLDDVDI KEAAKATTGK VVNNTGQVCT AGTRVLVPNK IKDAFLAELK
EQFSQVRVGN PREDGTQVGP IISKKQFDQV QNYINKGIEE GAELFYGGPG KPEGLEKGYF
ARPTIFINVD NQMTIAQEEI FGPVMSVITY NDLDEAIQIA NDTKYGLAGY VIGKDKETLH
KVARSIEAGT VEINEAGRKP DLPFGGYKQS GLGREWGDYG IEEFLEVKSI AGYFK
