FSD2_MOUSE
ID FSD2_MOUSE Reviewed; 716 AA.
AC Q8BZ52; A0A140T8J1; E9QMV0;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 3.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Fibronectin type III and SPRY domain-containing protein 2;
DE AltName: Full=Minispryn {ECO:0000303|PubMed:28740084};
DE AltName: Full=SPRY domain-containing protein 1;
GN Name=Fsd2; Synonyms=Spryd1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP TISSUE SPECIFICITY, INTERACTION WITH CMYA5, IDENTIFICATION IN A COMPLEX
RP WITH CMYA5 AND RYR2, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=28740084; DOI=10.1038/s41598-017-06395-6;
RA Benson M.A., Tinsley C.L., Waite A.J., Carlisle F.A., Sweet S.M.M.,
RA Ehler E., George C.H., Lai F.A., Martin-Rendon E., Blake D.J.;
RT "Ryanodine receptors are part of the myospryn complex in cardiac muscle.";
RL Sci. Rep. 7:6312-6312(2017).
CC -!- SUBUNIT: Interacts with CMYA5 (PubMed:28740084). In cardiac muscles,
CC identified in a complex composed of FSD2, CMYA5 and RYR2
CC (PubMed:28740084). {ECO:0000269|PubMed:28740084}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:H0UZ81}.
CC Sarcoplasmic reticulum {ECO:0000250|UniProtKB:H0UZ81}. Cytoplasm,
CC perinuclear region {ECO:0000250|UniProtKB:H0UZ81}. Note=In skeletal
CC muscles and striated muscles flanks Z-disks. Partially colocalizes with
CC RYR2 in the sarcoplasmic reticulum. {ECO:0000250|UniProtKB:H0UZ81}.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in heart and skeletal
CC muscle (at protein level). {ECO:0000269|PubMed:28740084}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC29530.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK036674; BAC29530.1; ALT_INIT; mRNA.
DR EMBL; AC140366; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC167122; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS80743.1; -.
DR RefSeq; NP_766492.2; NM_172904.2.
DR AlphaFoldDB; Q8BZ52; -.
DR SMR; Q8BZ52; -.
DR STRING; 10090.ENSMUSP00000047775; -.
DR PhosphoSitePlus; Q8BZ52; -.
DR PaxDb; Q8BZ52; -.
DR PRIDE; Q8BZ52; -.
DR ProteomicsDB; 266871; -.
DR ProteomicsDB; 328483; -.
DR Antibodypedia; 55562; 119 antibodies from 21 providers.
DR DNASU; 244091; -.
DR Ensembl; ENSMUST00000042318; ENSMUSP00000047775; ENSMUSG00000038663.
DR GeneID; 244091; -.
DR KEGG; mmu:244091; -.
DR UCSC; uc009icd.1; mouse.
DR CTD; 123722; -.
DR MGI; MGI:2444310; Fsd2.
DR VEuPathDB; HostDB:ENSMUSG00000038663; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000158441; -.
DR HOGENOM; CLU_022856_0_0_1; -.
DR InParanoid; Q8BZ52; -.
DR OMA; HFQHYVH; -.
DR OrthoDB; 508782at2759; -.
DR PhylomeDB; Q8BZ52; -.
DR TreeFam; TF315216; -.
DR BioGRID-ORCS; 244091; 5 hits in 65 CRISPR screens.
DR ChiTaRS; Fsd2; mouse.
DR PRO; PR:Q8BZ52; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8BZ52; protein.
DR Bgee; ENSMUSG00000038663; Expressed in interventricular septum and 84 other tissues.
DR Genevisible; Q8BZ52; MM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
DR GO; GO:0016529; C:sarcoplasmic reticulum; ISS:UniProtKB.
DR CDD; cd00063; FN3; 2.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR003879; Butyrophylin_SPRY.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR006574; PRY.
DR InterPro; IPR003877; SPRY_dom.
DR Pfam; PF00041; fn3; 1.
DR Pfam; PF13765; PRY; 1.
DR Pfam; PF00622; SPRY; 1.
DR PRINTS; PR01407; BUTYPHLNCDUF.
DR SMART; SM00060; FN3; 2.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49265; SSF49265; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS50853; FN3; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Nucleus; Reference proteome; Repeat;
KW Sarcoplasmic reticulum.
FT CHAIN 1..716
FT /note="Fibronectin type III and SPRY domain-containing
FT protein 2"
FT /id="PRO_0000317363"
FT DOMAIN 342..437
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 438..531
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 513..711
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT COILED 229..284
FT /evidence="ECO:0000255"
FT CONFLICT 1
FT /note="M -> L (in Ref. 1; BAC29530)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="E -> G (in Ref. 1; BAC29530)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 716 AA; 81473 MW; 6D64536F7DF917BB CRC64;
MEEEAEEESG LGRSAAPKDF HFYHMDLYDS EDRLQLFPGD SSRIRREVTQ AEDDRELGDE
FVDEHRLGTL GYPSYGMRRR DPGREPRDWG EAAEAEDLGY GGGQGPLDQC QDLREAYRYT
HGRASEEYEC YVIPEEEDEE EPADVFCITC KTPVRTVEKD FDTHKEHEVT PISKALEHAK
DEVHKNMCKL EQQIIEMENF ASHLEEVFIT VEENFGRQEQ NFECHYNGIL ETLAQKYEEK
IQALGEKKRE KLEALYGQLV SCGENLDACR ELMETVEEMC HEEKVEFLKD AVAMTDRLGK
FLKTKTDVEL SAQPEFEDQT LDFSDVEQLM DAINTIPAPS APVINPQAPN SATGSSVRVC
WSLYSDDTVE SYQLSYRPVQ DSSSGKDRAE FTMMVKETYC SVTNLEPNTQ YEFWVIAQNR
TGPSPCSEHA VYMTAPSPPS IKTEAIRSCE EAVLICWESG NLNPVDSYTV ELIQAETPEA
SGVTESVVGI PTCESLIQLQ PRHSYTIYVR ALNVGGTSAR SEPATVHTTG SYFQLNKDTC
HPWLTISEDG FTVVRSEKKS FRKELPPSKT QFTRCVAVMG NLIPVRGRHY WEVEVAEHLD
YTVGVACEDV PKQEDLGANS LSWCMRHTFV SKRHRYEFLH NKMTPDIRIT VAPRKIGVLL
DYENAKLSFF NVDIAQHLYT FSCQLHQFVH PCFSLEKSGC LKICNGISMP KHVTFF
