ID   ALDH_VIBC3              Reviewed;         506 AA.
AC   A5F3A7; C3LYI6; P23240; Q9KTS0;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Aldehyde dehydrogenase;
DE            EC=1.2.1.3;
GN   Name=aldA; Synonyms=aldA-1; OrderedLocusNames=VC0395_A0344, VC395_0836;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11179381; DOI=10.1128/iai.69.3.1947-1952.2001;
RA   Karaolis D.K.R., Lan R., Kaper J.B., Reeves P.R.;
RT   "Comparison of Vibrio cholerae pathogenicity islands in sixth and seventh
RT   pandemic strains.";
RL   Infect. Immun. 69:1947-1952(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
CC   -!- FUNCTION: May be involved in V.cholerae virulence, as its expression is
CC       under the control of ToxR, a transcriptional activator of several genes
CC       associated with virulence. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.3;
CC   -!- PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol:
CC       step 2/2.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABQ21488.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=ACP08852.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF325733; AAK20747.1; -; Genomic_DNA.
DR   EMBL; CP000627; ABQ21488.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP001235; ACP08852.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000640055.1; NZ_JAACZH010000023.1.
DR   AlphaFoldDB; A5F3A7; -.
DR   SMR; A5F3A7; -.
DR   STRING; 345073.VC395_0836; -.
DR   EnsemblBacteria; ABQ21488; ABQ21488; VC0395_A0344.
DR   GeneID; 57739523; -.
DR   KEGG; vco:VC0395_A0344; -.
DR   KEGG; vcr:VC395_0836; -.
DR   PATRIC; fig|345073.21.peg.807; -.
DR   eggNOG; COG1012; Bacteria.
DR   HOGENOM; CLU_005391_0_2_6; -.
DR   UniPathway; UPA00780; UER00768.
DR   Proteomes; UP000000249; Chromosome 2.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0043878; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006068; P:ethanol catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase; Virulence.
FT   CHAIN           1..506
FT                   /note="Aldehyde dehydrogenase"
FT                   /id="PRO_0000321860"
FT   ACT_SITE        262
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        301
FT                   /evidence="ECO:0000250"
FT   BINDING         240..245
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   506 AA;  55885 MW;  69E7F561D6CFED07 CRC64;
     MIYPIPNSET STVHFKDVYD NYIGGQWMKP HSGEYFSNTS PVNGLVFCRV ARSSSQDVEL
     ALDAAHNALE SWSTTSAVER SNILLRIADR IESNLETLAI VESWDNGKPI RETLAADLPL
     TIDHFRYFAA CIRSQEGAAS ELDSRTLTYH LPEPIGVVGQ IIPWNFPLLM AAWKLAPALA
     AGCTVVLKPA EQTPVSILFL MEIIGDLIPA GVINVVNGFG SEAGNALATS QRIDKLAFTG
     STEIGNHILK CAADNLIPST IELGGKSPNI YFPDIFSHED QYLDKCIEGA LLAFFNQGEV
     CTCPSRILVH ESIYEKFIAK IIERVALIKQ GNPLDTETQI GAQVSKEQYD KILGYIQIGK
     DEGAELIFGG HPNNQENYLS GGYYIKPTLF FGHNQMHIFQ EEIFGPVIAI TKFKDEIEAL
     HLANDTVYGL GAGVWTRDIN IAHRMAKNIK AGRVWVNCYH AYPAHAAFGG YKKSGIGRET
     HKLTLSHYQN IKNVLISHEI HPLGLF