FSIP1_MOUSE
ID FSIP1_MOUSE Reviewed; 435 AA.
AC Q9D3V5; Q8R4I9; Q9DAD8;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Fibrous sheath-interacting protein 1;
GN Name=Fsip1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), POSSIBLE INTERACTION WITH AKAP4,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=129/Sv; TISSUE=Testis;
RX PubMed=12606363; DOI=10.1095/biolreprod.102.013466;
RA Brown P.R., Miki K., Harper D.B., Eddy E.M.;
RT "A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the
RT sperm flagellum.";
RL Biol. Reprod. 68:2241-2248(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: May interact with AKAP4. {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9D3V5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9D3V5-2; Sequence=VSP_030430, VSP_030431;
CC -!- TISSUE SPECIFICITY: Detected in male germ cells and testis.
CC {ECO:0000269|PubMed:12606363}.
CC -!- DEVELOPMENTAL STAGE: First detected in testis of 18 day old mice.
CC {ECO:0000269|PubMed:12606363}.
CC -!- SIMILARITY: Belongs to the FSIP1 family. {ECO:0000305}.
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DR EMBL; AF448787; AAM18537.1; -; mRNA.
DR EMBL; AK005924; BAB24319.1; -; mRNA.
DR EMBL; AK017026; BAB30556.1; -; mRNA.
DR EMBL; AL845495; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC125024; AAI25025.1; -; mRNA.
DR CCDS; CCDS16573.1; -. [Q9D3V5-1]
DR RefSeq; NP_082035.1; NM_027759.3. [Q9D3V5-1]
DR AlphaFoldDB; Q9D3V5; -.
DR SMR; Q9D3V5; -.
DR STRING; 10090.ENSMUSP00000028821; -.
DR PhosphoSitePlus; Q9D3V5; -.
DR MaxQB; Q9D3V5; -.
DR PaxDb; Q9D3V5; -.
DR PRIDE; Q9D3V5; -.
DR ProteomicsDB; 267412; -. [Q9D3V5-1]
DR ProteomicsDB; 267413; -. [Q9D3V5-2]
DR Antibodypedia; 52142; 229 antibodies from 20 providers.
DR Ensembl; ENSMUST00000028820; ENSMUSP00000028820; ENSMUSG00000027344. [Q9D3V5-2]
DR Ensembl; ENSMUST00000028821; ENSMUSP00000028821; ENSMUSG00000027344. [Q9D3V5-1]
DR GeneID; 71313; -.
DR KEGG; mmu:71313; -.
DR UCSC; uc008lrs.2; mouse. [Q9D3V5-1]
DR CTD; 161835; -.
DR MGI; MGI:1918563; Fsip1.
DR VEuPathDB; HostDB:ENSMUSG00000027344; -.
DR eggNOG; ENOG502RXFB; Eukaryota.
DR GeneTree; ENSGT00390000013879; -.
DR HOGENOM; CLU_031884_1_0_1; -.
DR InParanoid; Q9D3V5; -.
DR OMA; PCKVETA; -.
DR OrthoDB; 608640at2759; -.
DR PhylomeDB; Q9D3V5; -.
DR TreeFam; TF351151; -.
DR BioGRID-ORCS; 71313; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Fsip1; mouse.
DR PRO; PR:Q9D3V5; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D3V5; protein.
DR Bgee; ENSMUSG00000027344; Expressed in seminiferous tubule of testis and 53 other tissues.
DR ExpressionAtlas; Q9D3V5; baseline and differential.
DR Genevisible; Q9D3V5; MM.
DR GO; GO:0031514; C:motile cilium; IC:MGI.
DR InterPro; IPR026246; Fsip1.
DR PANTHER; PTHR22012; PTHR22012; 1.
DR Pfam; PF15554; FSIP1; 1.
DR PRINTS; PR02075; FIBSHEATHIP1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..435
FT /note="Fibrous sheath-interacting protein 1"
FT /id="PRO_0000314920"
FT REGION 1..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 108..154
FT /evidence="ECO:0000255"
FT COMPBIAS 13..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..92
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 357..393
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 87
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H16"
FT MOD_RES 88
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q66H16"
FT VAR_SEQ 189..221
FT /note="AEALHCEFEDALFSVFHTQIPPETYENLMEKDF -> GQPSLIMEDYSFNWY
FT RFEWLNLFRSFISIKQD (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030430"
FT VAR_SEQ 222..435
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030431"
FT CONFLICT 27
FT /note="R -> Q (in Ref. 1; AAM18537 and 4; AAI25025)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="S -> P (in Ref. 1; AAM18537 and 4; AAI25025)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="A -> V (in Ref. 1; AAM18537 and 4; AAI25025)"
FT /evidence="ECO:0000305"
FT CONFLICT 381
FT /note="R -> Q (in Ref. 1; AAM18537 and 4; AAI25025)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 435 AA; 48968 MW; 8E2C146380BB48C0 CRC64;
MPMDIIKGNL DGISKPASSS RSRPGSRSSN GSLEVLTPEP GSVKIDMVNK LNSGQEGHTS
NSGVEERRNS NDAKWADDSK TKPAKESSDE DPDMPQPQAT PEHSDDPKLE ETNAVLQNAI
RKMHRLDKLL AKKQCREKEV KKQGLEMRVK LWEELKSAKN TEDLENDEEL GNTKKFLCLT
SESAGKAAAE ALHCEFEDAL FSVFHTQIPP ETYENLMEKD FTCDVEKNEP LIITEKQPFS
NTEAIEPRSE DSQGFIRQNA EHSQDFIKRN IELAKHSRSP VVMVEGEKKR LDELLQGLDD
ADSGLSSAEG DQCGWLVPGE GYTLAATESQ QLAEIDIKLQ ELSVDSPTIF SLESQSHKGD
MECDANEERN TEPTPGEKIL RDRKEQRDRE SRLRAIDGKL KELSEQVEEC PMITPSKRNE
RITWRWLLAK ILEPE
