位置:首页 > 蛋白库 > FSQG_ASPFU
FSQG_ASPFU
ID   FSQG_ASPFU              Reviewed;         588 AA.
AC   Q4WD48;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Phenol 2-monooxygenase fsqG {ECO:0000303|PubMed:27065235};
DE            EC=1.14.13.- {ECO:0000305|PubMed:27065235};
DE   AltName: Full=Fumipyrrole biosynthesis protein F {ECO:0000303|PubMed:25582336};
DE   AltName: Full=Fumisoquins biosynthesis protein G {ECO:0000303|PubMed:27065235};
GN   Name=fsqG {ECO:0000303|PubMed:27065235};
GN   Synonyms=fmpF {ECO:0000303|PubMed:25582336}; ORFNames=AFUA_6G03490;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=25582336; DOI=10.1111/mmi.12926;
RA   Macheleidt J., Scherlach K., Neuwirth T., Schmidt-Heck W., Strassburger M.,
RA   Spraker J., Baccile J.A., Schroeder F.C., Keller N.P., Hertweck C.,
RA   Heinekamp T., Brakhage A.A.;
RT   "Transcriptome analysis of cyclic AMP-dependent protein kinase A-regulated
RT   genes reveals the production of the novel natural compound fumipyrrole by
RT   Aspergillus fumigatus.";
RL   Mol. Microbiol. 96:148-162(2015).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=27065235; DOI=10.1038/nchembio.2061;
RA   Baccile J.A., Spraker J.E., Le H.H., Brandenburger E., Gomez C., Bok J.W.,
RA   Macheleidt J., Brakhage A.A., Hoffmeister D., Keller N.P., Schroeder F.C.;
RT   "Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus
RT   fumigatus.";
RL   Nat. Chem. Biol. 12:419-424(2016).
RN   [4]
RP   FUNCTION.
RX   PubMed=30194285; DOI=10.1074/jbc.ra118.004227;
RA   Lahham M., Pavkov-Keller T., Fuchs M., Niederhauser J., Chalhoub G.,
RA   Daniel B., Kroutil W., Gruber K., Macheroux P.;
RT   "Oxidative cyclization of N-methyl-dopa by a fungal flavoenzyme of the
RT   amine oxidase family.";
RL   J. Biol. Chem. 293:17021-17032(2018).
CC   -!- FUNCTION: Phenol 2-monooxygenase; part of the gene cluster that
CC       mediates the biosynthesis of the isoquinoline alkaloids fumisoquin A,
CC       fumisoquin B and fumisoquin C; as well as small amounts of fumipyrrole
CC       as a shunt metabolite (PubMed:25582336, PubMed:27065235). The products
CC       of the cluster lead to a brown coloration and are important for growth
CC       and conidiation (PubMed:25582336). The nonribosomal peptide synthetase-
CC       like protein fsqF, which lacks a canonical condensation domain, is
CC       required for addition of a serine-derived dehydroalanine moiety to
CC       activated tyrosine but is not essential for the subsequent steps
CC       leading to isoquinoline formation (PubMed:27065235). A different
CC       enzyme, most likely the ATP-grasp enzyme fsqD, is responsible for
CC       activation of tyrosine (Probable). Three additional enzymes encoded by
CC       the fsq cluster, the N-methyltransferase fsqC, the phenol 2-
CC       monooxygenase fsqG and the FAD-dependent oxidase fsqB, catalyze the
CC       formation of the isoquinoline ring system in the fumisoquins
CC       (PubMed:27065235, PubMed:30194285). FsqB converts the fspF thiolation
CC       domain-bound (2S,4S,5S)-2-amino-6-(3,4-dihydroxyphenyl)-4-hydroxy-5-
CC       (methylamino)hexanoyl into isoquinoline (PubMed:27065235,
CC       PubMed:30194285). The cyclization most likely proceeds via a two-step
CC       mechanism, beginning with FAD-dependent oxidation of the methyl group
CC       to an iminium species followed by electrophilic attack on the
CC       deprotonated phenol (Probable). {ECO:0000269|PubMed:25582336,
CC       ECO:0000269|PubMed:27065235, ECO:0000269|PubMed:30194285,
CC       ECO:0000305|PubMed:27065235}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:P15245};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:25582336}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P15245}.
CC   -!- INDUCTION: Expression is positively regulated by the fumisoquins
CC       biosynthesis specific transcription factor fsqA (PubMed:25582336).
CC       {ECO:0000269|PubMed:25582336, ECO:0000269|PubMed:27065235}.
CC   -!- DISRUPTION PHENOTYPE: Leads to complete abolishment of isoquinoline
CC       alkaloid production but accumulates a series of benzyl pyrroles,
CC       including fumipyrrole. {ECO:0000269|PubMed:27065235}.
CC   -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAHF01000012; EAL85690.1; -; Genomic_DNA.
DR   RefSeq; XP_747728.1; XM_742635.1.
DR   AlphaFoldDB; Q4WD48; -.
DR   SMR; Q4WD48; -.
DR   STRING; 746128.CADAFUBP00009214; -.
DR   EnsemblFungi; EAL85690; EAL85690; AFUA_6G03490.
DR   GeneID; 3505023; -.
DR   KEGG; afm:AFUA_6G03490; -.
DR   eggNOG; KOG3855; Eukaryota.
DR   HOGENOM; CLU_009665_9_2_1; -.
DR   InParanoid; Q4WD48; -.
DR   OMA; ANWFRNS; -.
DR   OrthoDB; 366744at2759; -.
DR   Proteomes; UP000002530; Chromosome 6.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR   Gene3D; 3.40.30.20; -; 1.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR012941; Phe_hydrox_C_dim_dom.
DR   InterPro; IPR038220; PHOX_C_sf.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   Pfam; PF07976; Phe_hydrox_dim; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..588
FT                   /note="Phenol 2-monooxygenase fsqG"
FT                   /id="PRO_0000438874"
FT   BINDING         9..38
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         17..18
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         37..39
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         45..50
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         49
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         232
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         232
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         289..299
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         299
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
FT   BINDING         309..313
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P15245"
SQ   SEQUENCE   588 AA;  65844 MW;  BEFE9CF8C3077E57 CRC64;
     MSNASDHVDV LIIGAGPAGL TTANSFNGSN CRVRLIDWKP APLETGRADG LKSISLEVLD
     SFGIGDRVLN DCQPCEEIVL WNPGKDGVIA RTMTIPDKVE ELGQIERVMV ENLYRHKTVQ
     VNWNTQPVRL HIAPVTKDEP EAHPLTITVQ NKETLGQETI RAKYVVGADG AHSWLRKYLN
     IGFSGDVTDS TWGVMNLVPK TDFPDIRKVF VVHSRAGTVM GVPREDKLVR LYISMDGGNR
     HTSIDAKSIT AENLLQAARA ILAPYRLDAA RIPWWSAYCV GQRVADEFAR HNRIFLAGDA
     VHTHSPKAGQ GMNTSIQDGY NIGWKLRYCL EQKASPALLS TYQTERRPIA QALIDFDRKY
     LESFTRRDIT HQEFLEAYLA GQRFTTGIQI QYPPSLIVTA KHLHAPSHPL ATNLAPGKRL
     PDFQMVNQSD AVPIQAYHRF TSDGRFRLLV FPGDISQALA FGRFSRLGDW LTSHLPPSSG
     LEIITIHGAR RADVELMDLH PAFRPWSDEE GWNYWTVYAD DDSYHKGHGH VYERCGISKE
     DGVLVLLRPD GYISLIASFD ETHELIDFFD GLQSGPRTVP QPERRANL
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024