FSTL4_MOUSE
ID FSTL4_MOUSE Reviewed; 841 AA.
AC Q5STE3; A2RT35; Q5DU03;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Follistatin-related protein 4;
DE AltName: Full=Follistatin-like protein 4;
DE AltName: Full=m-D/Bsp120I 1-1;
DE Flags: Precursor;
GN Name=Fstl4; Synonyms=Kiaa1061;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Kato A., Noda M.;
RT "Identification of m-D/Bsp120I 1-1.";
RL Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 146-841.
RC TISSUE=Brain;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
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DR EMBL; AF374459; AAQ02777.1; -; mRNA.
DR EMBL; AL596382; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL604002; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL662836; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL662855; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC132353; AAI32354.1; -; mRNA.
DR EMBL; AK220367; BAD90427.1; -; mRNA.
DR CCDS; CCDS24673.1; -.
DR RefSeq; NP_796033.2; NM_177059.3.
DR AlphaFoldDB; Q5STE3; -.
DR SMR; Q5STE3; -.
DR STRING; 10090.ENSMUSP00000042007; -.
DR GlyGen; Q5STE3; 1 site.
DR PhosphoSitePlus; Q5STE3; -.
DR PaxDb; Q5STE3; -.
DR PRIDE; Q5STE3; -.
DR ProteomicsDB; 266875; -.
DR Antibodypedia; 26231; 41 antibodies from 14 providers.
DR DNASU; 320027; -.
DR Ensembl; ENSMUST00000036796; ENSMUSP00000042007; ENSMUSG00000036264.
DR GeneID; 320027; -.
DR KEGG; mmu:320027; -.
DR UCSC; uc007ivp.1; mouse.
DR CTD; 23105; -.
DR MGI; MGI:2443199; Fstl4.
DR VEuPathDB; HostDB:ENSMUSG00000036264; -.
DR eggNOG; ENOG502QPNV; Eukaryota.
DR GeneTree; ENSGT00940000158076; -.
DR HOGENOM; CLU_007849_0_0_1; -.
DR InParanoid; Q5STE3; -.
DR OMA; LNFMALE; -.
DR OrthoDB; 1065760at2759; -.
DR PhylomeDB; Q5STE3; -.
DR TreeFam; TF350473; -.
DR BioGRID-ORCS; 320027; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Fstl4; mouse.
DR PRO; PR:Q5STE3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5STE3; protein.
DR Bgee; ENSMUSG00000036264; Expressed in secondary oocyte and 36 other tissues.
DR Genevisible; Q5STE3; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030141; C:secretory granule; IDA:MGI.
DR GO; GO:0048403; F:brain-derived neurotrophic factor binding; IDA:MGI.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0031549; P:negative regulation of brain-derived neurotrophic factor receptor signaling pathway; IMP:MGI.
DR GO; GO:0048671; P:negative regulation of collateral sprouting; IMP:MGI.
DR GO; GO:0061000; P:negative regulation of dendritic spine development; IMP:MGI.
DR GO; GO:0048670; P:regulation of collateral sprouting; IGI:MGI.
DR GO; GO:0048731; P:system development; IEA:UniProt.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF07648; Kazal_2; 1.
DR SMART; SM00409; IG; 2.
DR SMART; SM00408; IGc2; 2.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS50835; IG_LIKE; 2.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 2: Evidence at transcript level;
KW Calcium; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Metal-binding; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..841
FT /note="Follistatin-related protein 4"
FT /id="PRO_0000251926"
FT DOMAIN 80..134
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DOMAIN 173..208
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 225..247
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 250..336
FT /note="Ig-like 1"
FT DOMAIN 340..425
FT /note="Ig-like 2"
FT REGION 29..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 186
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 188
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 190
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 192
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 197
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 225
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 227
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 229
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 231
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 236
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT CARBOHYD 317
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 86..118
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 92..111
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 100..132
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 269..320
FT /evidence="ECO:0000250"
FT DISULFID 361..412
FT /evidence="ECO:0000250"
FT CONFLICT 251
FT /note="E -> K (in Ref. 4; BAD90427)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 841 AA; 92579 MW; 8FE6441B8EB8BA76 CRC64;
MKPGGFWPHL ALLGVSLPAV LGWMDQGASR SPNMVPGESQ AEETRGFEVT RREGLPSPGL
SASCGKKLCS HGSRCLLNRT TGQPSCQCLE VCRPRYMPVC GSDGRLYGNH CELRRAACLL
GKRIVSVHSK DCFLKGDMCT MAGYARLKNV LLALQSRRQP LPQGTPRQSL ASQKRLLVES
LFKDLDADGN GHLGSLELAQ YVLKEQDMDG SLNRCSPSDL LRFDDYNSDG SLTLGEFYTA
FQVIQLSLAP EDKVSVTTVT VGLSTVLTCA IRGDLRPPII WKRNGLTLSF LGLEDINDFG
EDGSLYITKV TTVHMGNYTC HALGHEQLVQ THVLQVNVPP VIRVYPETQA QEPGVAASLR
CHAEGIPLPR IIWLKNGMDV STQMSKQLSL LANGSELHIG SVRYEDTGAY TCIAKNEVGV
DEDISSLFIE DSARKTLANI LWREEGLSVG NMFYVFAEDG IVVIHPVDCE VQRRLKPTEK
IFMSYEEICP RVEGDATQPC QWASAVNVRN RYIYVAQPAL NRVLVVDVQA QKVLQSIGVD
PLPVKLSYDK SHDQVWVLSW GDMHKSQPSL QVITEASTGQ GQHLIRTPFA GVDNFFIPPT
NLIINHIRFG FIFNKTDPAV HKVDLETLMA LKTISLRHYG CMPQAMAHTH LGGYFFVQCQ
QDTPTSTGPQ LLIDSVTDSV LGPNSDITGT PHVSPDGRFI VSVSNKGPWL HVQEVTVRGE
IQTLYDLKIN PGISDLAFQH SFTEGSQYNA YATLDKEPDL LFLELSTGKM GRLKNLKEPP
RGPAPTWGGP RRVLRDSGLF GQYLLTPAQE SLFLVNGRQN ALRCEVSGIK GAATVVWVGE
V
