FSTL5_HUMAN
ID FSTL5_HUMAN Reviewed; 847 AA.
AC Q8N475; E9PCP6; Q9NSW7; Q9ULF7;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Follistatin-related protein 5;
DE AltName: Full=Follistatin-like protein 5;
DE Flags: Precursor;
GN Name=FSTL5; Synonyms=KIAA1263;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=10574462; DOI=10.1093/dnares/6.5.337;
RA Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XV. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 6:337-345(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 75-847 (ISOFORM 1).
RC TISSUE=Kidney;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [6]
RP VARIANT [LARGE SCALE ANALYSIS] ILE-92.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8N475-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N475-2; Sequence=VSP_046215;
CC Name=3;
CC IsoId=Q8N475-3; Sequence=VSP_046215, VSP_046216;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA86577.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB033089; BAA86577.1; ALT_INIT; mRNA.
DR EMBL; AK315152; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC023136; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC079227; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC084281; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC096717; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC104793; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC105251; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC108163; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC036502; AAH36502.1; -; mRNA.
DR EMBL; AL137695; CAB70877.1; -; mRNA.
DR CCDS; CCDS3802.1; -. [Q8N475-1]
DR CCDS; CCDS47157.1; -. [Q8N475-3]
DR CCDS; CCDS47158.1; -. [Q8N475-2]
DR PIR; T46283; T46283.
DR RefSeq; NP_001121899.1; NM_001128427.2. [Q8N475-2]
DR RefSeq; NP_001121900.1; NM_001128428.2. [Q8N475-3]
DR RefSeq; NP_064501.2; NM_020116.4. [Q8N475-1]
DR AlphaFoldDB; Q8N475; -.
DR SMR; Q8N475; -.
DR BioGRID; 121216; 2.
DR IntAct; Q8N475; 4.
DR STRING; 9606.ENSP00000305334; -.
DR MEROPS; I01.977; -.
DR GlyGen; Q8N475; 2 sites.
DR iPTMnet; Q8N475; -.
DR PhosphoSitePlus; Q8N475; -.
DR BioMuta; FSTL5; -.
DR DMDM; 62510692; -.
DR EPD; Q8N475; -.
DR MassIVE; Q8N475; -.
DR MaxQB; Q8N475; -.
DR PaxDb; Q8N475; -.
DR PeptideAtlas; Q8N475; -.
DR PRIDE; Q8N475; -.
DR ProteomicsDB; 19487; -.
DR ProteomicsDB; 71893; -. [Q8N475-1]
DR Antibodypedia; 28231; 98 antibodies from 18 providers.
DR DNASU; 56884; -.
DR Ensembl; ENST00000306100.10; ENSP00000305334.4; ENSG00000168843.14. [Q8N475-1]
DR Ensembl; ENST00000379164.8; ENSP00000368462.4; ENSG00000168843.14. [Q8N475-2]
DR Ensembl; ENST00000427802.2; ENSP00000389270.2; ENSG00000168843.14. [Q8N475-3]
DR GeneID; 56884; -.
DR KEGG; hsa:56884; -.
DR MANE-Select; ENST00000306100.10; ENSP00000305334.4; NM_020116.5; NP_064501.2.
DR UCSC; uc003iqh.5; human. [Q8N475-1]
DR CTD; 56884; -.
DR DisGeNET; 56884; -.
DR GeneCards; FSTL5; -.
DR HGNC; HGNC:21386; FSTL5.
DR HPA; ENSG00000168843; Group enriched (brain, retina).
DR neXtProt; NX_Q8N475; -.
DR OpenTargets; ENSG00000168843; -.
DR PharmGKB; PA134978045; -.
DR VEuPathDB; HostDB:ENSG00000168843; -.
DR eggNOG; ENOG502QPNV; Eukaryota.
DR GeneTree; ENSGT00940000156495; -.
DR HOGENOM; CLU_007849_1_0_1; -.
DR InParanoid; Q8N475; -.
DR OMA; KYGYVTH; -.
DR OrthoDB; 1065760at2759; -.
DR PhylomeDB; Q8N475; -.
DR TreeFam; TF350473; -.
DR PathwayCommons; Q8N475; -.
DR SignaLink; Q8N475; -.
DR BioGRID-ORCS; 56884; 18 hits in 1076 CRISPR screens.
DR ChiTaRS; FSTL5; human.
DR GenomeRNAi; 56884; -.
DR Pharos; Q8N475; Tbio.
DR PRO; PR:Q8N475; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q8N475; protein.
DR Bgee; ENSG00000168843; Expressed in cerebellar vermis and 100 other tissues.
DR Genevisible; Q8N475; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR Pfam; PF07648; Kazal_2; 1.
DR SMART; SM00409; IG; 2.
DR SMART; SM00408; IGc2; 2.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; SSF100895; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF48726; SSF48726; 2.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS50835; IG_LIKE; 2.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Disulfide bond; Glycoprotein;
KW Immunoglobulin domain; Metal-binding; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..847
FT /note="Follistatin-related protein 5"
FT /id="PRO_0000010118"
FT DOMAIN 83..135
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DOMAIN 175..210
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 211..246
FT /note="EF-hand 2"
FT /evidence="ECO:0000305"
FT DOMAIN 250..337
FT /note="Ig-like 1"
FT DOMAIN 341..426
FT /note="Ig-like 2"
FT BINDING 188
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 190
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 192
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 199
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 226
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT BINDING 228
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT BINDING 230
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT BINDING 232
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT BINDING 237
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10142"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 394
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 89..119
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 93..112
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 101..133
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 270..321
FT /evidence="ECO:0000250"
FT DISULFID 362..413
FT /evidence="ECO:0000250"
FT VAR_SEQ 43
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:10574462,
FT ECO:0000303|PubMed:14702039"
FT /id="VSP_046215"
FT VAR_SEQ 438..447
FT /note="LANILWREEG -> R (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_046216"
FT VARIANT 92
FT /note="L -> I (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs747628234)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036135"
FT VARIANT 711
FT /note="D -> Y (in dbSNP:rs3749598)"
FT /id="VAR_049092"
FT VARIANT 815
FT /note="K -> E (in dbSNP:rs17040982)"
FT /id="VAR_049093"
FT CONFLICT 145
FT /note="Y -> C (in Ref. 5; CAB70877)"
FT /evidence="ECO:0000305"
FT CONFLICT 159
FT /note="K -> R (in Ref. 5; CAB70877)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="G -> D (in Ref. 5; CAB70877)"
FT /evidence="ECO:0000305"
FT CONFLICT 293
FT /note="D -> G (in Ref. 5; CAB70877)"
FT /evidence="ECO:0000305"
FT CONFLICT 492
FT /note="K -> I (in Ref. 5; CAB70877)"
FT /evidence="ECO:0000305"
FT CONFLICT 560
FT /note="S -> G (in Ref. 2; AK315152)"
FT /evidence="ECO:0000305"
FT CONFLICT 567
FT /note="T -> S (in Ref. 4; AAH36502)"
FT /evidence="ECO:0000305"
FT CONFLICT 631
FT /note="L -> I (in Ref. 4; AAH36502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 847 AA; 95751 MW; AA8ECA182FF6CDB6 CRC64;
MFKCWSVVLV LGFIFLESEG RPTKEGGYGL KSYQPLMRLR HKQEKNQESS RVKGFMIQDG
PFGSCENKYC GLGRHCVTSR ETGQAECACM DLCKRHYKPV CGSDGEFYEN HCEVHRAACL
KKQKITIVHN EDCFFKGDKC KTTEYSKMKN MLLDLQNQKY IMQENENPNG DDISRKKLLV
DQMFKYFDAD SNGLVDINEL TQVIKQEELG KDLFDCTLYV LLKYDDFNAD KHLALEEFYR
AFQVIQLSLP EDQKLSITAA TVGQSAVLSC AIQGTLRPPI IWKRNNIILN NLDLEDINDF
GDDGSLYITK VTTTHVGNYT CYADGYEQVY QTHIFQVNVP PVIRVYPESQ AREPGVTASL
RCHAEGIPKP QLGWLKNGID ITPKLSKQLT LQANGSEVHI SNVRYEDTGA YTCIAKNEAG
VDEDISSLFV EDSARKTLAN ILWREEGLGI GNMFYVFYED GIKVIQPIEC EFQRHIKPSE
KLLGFQDEVC PKAEGDEVQR CVWASAVNVK DKFIYVAQPT LDRVLIVDVQ SQKVVQAVST
DPVPVKLHYD KSHDQVWVLS WGTLEKTSPT LQVITLASGN VPHHTIHTQP VGKQFDRVDD
FFIPTTTLII THMRFGFILH KDEAALQKID LETMSYIKTI NLKDYKCVPQ SLAYTHLGGY
YFIGCKPDST GAVSPQVMVD GVTDSVIGFN SDVTGTPYVS PDGHYLVSIN DVKGLVRVQY
ITIRGEIQEA FDIYTNLHIS DLAFQPSFTE AHQYNIYGSS STQTDVLFVE LSSGKVKMIK
SLKEPLKAEE WPWNRKNRQI QDSGLFGQYL MTPSKDSLFI LDGRLNKLNC EITEVEKGNT
VIWVGDA
