FSYA_DROME
ID FSYA_DROME Reviewed; 696 AA.
AC P25028; Q8T057; Q9W4W0; Q9W4W1;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 4.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Mitosis initiation protein fs(1)Ya;
GN Name=fs(1)Ya; ORFNames=CG2707;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Embryo;
RX PubMed=1986869; DOI=10.1016/0092-8674(91)90208-g;
RA Lin H., Wolfner M.F.;
RT "The Drosophila maternal-effect gene fs(1)Ya encodes a cell cycle-dependent
RT nuclear envelope component required for embryonic mitosis.";
RL Cell 64:49-62(1991).
RN [2]
RP SEQUENCE REVISION.
RA Liu J., Wolfner M.F.;
RL Submitted (JAN-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-478; THR-484 AND THR-489, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Cell cycle-dependent nuclear envelope component required for
CC embryonic mitosis.
CC -!- SUBCELLULAR LOCATION: Nucleus envelope. Nucleus, nucleoplasm.
CC Cytoplasm. Note=In the nuclear envelope during interphase to metaphase.
CC And in the nucleoplasm and cytoplasm during anaphase and telophase.
CC -!- DEVELOPMENTAL STAGE: Maternal protein synthesized during postoogenic
CC maturation and persisting throughout embryogenesis.
CC -!- MISCELLANEOUS: The OPA repeat-containing and the Ser/Thr-rich regions
CC might be involved in protein-protein interactions with the major
CC protein-rich layer of the nuclear envelope.
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DR EMBL; M38442; AAA65184.1; -; mRNA.
DR EMBL; AE014298; AAF45816.2; -; Genomic_DNA.
DR EMBL; AL021728; CAA16819.1; -; Genomic_DNA.
DR EMBL; AY069542; AAL39687.1; -; mRNA.
DR PIR; A38436; A38436.
DR PIR; T13648; T13648.
DR RefSeq; NP_477493.2; NM_058145.4.
DR AlphaFoldDB; P25028; -.
DR BioGRID; 57794; 2.
DR DIP; DIP-115N; -.
DR IntAct; P25028; 2.
DR MINT; P25028; -.
DR STRING; 7227.FBpp0070463; -.
DR iPTMnet; P25028; -.
DR PaxDb; P25028; -.
DR PRIDE; P25028; -.
DR DNASU; 31263; -.
DR EnsemblMetazoa; FBtr0070485; FBpp0070463; FBgn0000927.
DR GeneID; 31263; -.
DR KEGG; dme:Dmel_CG2707; -.
DR CTD; 31263; -.
DR FlyBase; FBgn0000927; fs(1)Ya.
DR VEuPathDB; VectorBase:FBgn0000927; -.
DR eggNOG; ENOG502SW8S; Eukaryota.
DR HOGENOM; CLU_015130_1_0_1; -.
DR InParanoid; P25028; -.
DR OMA; MFAKPKE; -.
DR OrthoDB; 1541424at2759; -.
DR PhylomeDB; P25028; -.
DR BioGRID-ORCS; 31263; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31263; -.
DR PRO; PR:P25028; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000927; Expressed in oocyte and 8 other tissues.
DR Genevisible; P25028; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:FlyBase.
DR GO; GO:0005652; C:nuclear lamina; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006325; P:chromatin organization; IMP:FlyBase.
DR GO; GO:0030261; P:chromosome condensation; IMP:FlyBase.
DR GO; GO:0006260; P:DNA replication; IGI:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0006997; P:nucleus organization; IMP:FlyBase.
DR GO; GO:0007344; P:pronuclear fusion; IMP:FlyBase.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Mitosis; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..696
FT /note="Mitosis initiation protein fs(1)Ya"
FT /id="PRO_0000087358"
FT REGION 245..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 336..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 448..696
FT /note="Rich in charged AA"
FT REGION 457..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..586
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 603..696
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 512..520
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000305"
FT MOTIF 534..538
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000305"
FT COMPBIAS 246..283
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..379
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 605..626
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 661..680
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 681..696
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 478
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 484
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 489
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 270
FT /note="Missing (in Ref. 5; CAA16819)"
FT /evidence="ECO:0000305"
FT CONFLICT 412
FT /note="K -> E (in Ref. 5; CAA16819)"
FT /evidence="ECO:0000305"
FT CONFLICT 673
FT /note="L -> P (in Ref. 1; AAA65184)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 696 AA; 77726 MW; C938FFFB3D9A5481 CRC64;
MSFSNVLIMR QPDEGKCHIC KRVFCCGKCR QKHQFKAHAI AVREPLGLRS AGGGIIEHRH
QMESGATTIY VFCPICERRP LLLREEMHGE LLAHIETCHL PLRCRKCQRN YTRVDDLREF
SKCVDQQQSC TDVTGATETS KATLKKAANS TAISTQTSPS VTPISLINMR WKAKSRVTHE
EFISDSVSSI RNLSSFSNSS IRRSIGQLGV NPSETMEKGK VIRSTSTPLH VESVFAKPKE
PITFNASTGG HVSSIYHEEP SPTPESNPVQ QQQQQQQPLQ QRAWKMGARN KMSAATPLRQ
VMSKSIQKAF VEHGGMMVHQ PPSAVVQRRV RLDLSEHSSH EAAGSSALDL RLSPAMRRTQ
SESSASEVNS GSSSSYSTSR NADLCKRQFL LSAQKLTTES IIITRTNSSS QKTSSTVYNS
CESVEIIRST SESAEVCHVP AITPIRVTGA GINKKQIKFE TPPKSSQQMR SNGEGDETKD
QFFTPEPGTP EIPERRHRQA IVPRQLSGEF SPKKDKPKEK GLAVMALISP PLQQPRVRPP
LRECRQQRVY SGVQDVGEPE VVDAEEEDEV FRPTNASTCN DKKLEAPNSG RLWSLMSSMM
RLPASLRGER EKDRDRDRDS DKENAGSGSL IRRCASIAGS LVRPSARDSS MEDQQCLKRK
RTQTLDSQYC SPLSPSSSSK RYRIRPREPI ERMRRQ
