ID   FTHS1_DESHY             Reviewed;         556 AA.
AC   Q251P8;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Formate--tetrahydrofolate ligase 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS 1 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs1 {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=DSY0205;
OS   Desulfitobacterium hafniense (strain Y51).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=138119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y51;
RX   PubMed=16513756; DOI=10.1128/jb.188.6.2262-2274.2006;
RA   Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA   Inatomi K., Furukawa K., Inui M., Yukawa H.;
RT   "Complete genome sequence of the dehalorespiring bacterium
RT   Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides
RT   ethenogenes 195.";
RL   J. Bacteriol. 188:2262-2274(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AP008230; BAE81994.1; -; Genomic_DNA.
DR   RefSeq; WP_005809743.1; NC_007907.1.
DR   AlphaFoldDB; Q251P8; -.
DR   SMR; Q251P8; -.
DR   STRING; 138119.DSY0205; -.
DR   EnsemblBacteria; BAE81994; BAE81994; DSY0205.
DR   KEGG; dsy:DSY0205; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001946; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..556
FT                   /note="Formate--tetrahydrofolate ligase 1"
FT                   /id="PRO_0000293034"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   556 AA;  60058 MW;  3ED979494B7BE038 CRC64;
     MKTDIEIAQE ATMKPITEIA QGLDLLEDEI ELYGKYKAKV NFSAWERLKD KPDAKLILVT
     AINPTPAGEG KTTTTVGLGQ AMSKIGKNAM IALREPSLGP CFGVKGGAAG GGYAQVVPME
     DINLHFTGDF HAITSTHNLL AALLDNHIQQ GNLLNIDPRQ IVFRRVMDMN DRALRKIVIG
     LGGRTEGIPR ENGFDITVAS EIMAILCLAK DLMDLKERFG RIVVAYTYDG KAITAHDLEA
     EGAMALLMKD AIKPNLVQTL ENTPVFIHGG PFANIAHGCN SVVATRMAMK LADYVITEAG
     FGADLGAEKF YDLKCRFAEL KPAATVIVAT VRALKMNGGV AKEDLGPENL EALAKGIVNL
     EKHIENIGKF GVPAVVAINR FPTDTDAELE FVAERCRQLG AEFALSEVFT KGGEGGIELA
     KAVLNIVDNK ESNFHVLYEL DLPIAKKIET ICKEVYGADG VNFTKEALTS MKKYEELGYG
     QLPICMAKTQ YSLTDDQNVL GRPSGFTITV RELRLSAGAG FLVAITGAIM TMPGLPKRPA
     ALRMDIDAAG RITGLF