FTHS1_RUBXD
ID FTHS1_RUBXD Reviewed; 576 AA.
AC Q1AXZ4;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Formate--tetrahydrofolate ligase 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS 1 {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS 1 {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs1 {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Rxyl_0766;
OS Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1).
OC Bacteria; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae;
OC Rubrobacter.
OX NCBI_TaxID=266117;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 9941 / NBRC 16129 / PRD-1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S.,
RA Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P.;
RT "Complete sequence of Rubrobacter xylanophilus DSM 9941.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000386; ABG03734.1; -; Genomic_DNA.
DR RefSeq; WP_011563752.1; NC_008148.1.
DR AlphaFoldDB; Q1AXZ4; -.
DR SMR; Q1AXZ4; -.
DR STRING; 266117.Rxyl_0766; -.
DR EnsemblBacteria; ABG03734; ABG03734; Rxyl_0766.
DR KEGG; rxy:Rxyl_0766; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_11; -.
DR OrthoDB; 177859at2; -.
DR PhylomeDB; Q1AXZ4; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000006637; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..576
FT /note="Formate--tetrahydrofolate ligase 1"
FT /id="PRO_0000293054"
FT BINDING 69..76
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 576 AA; 61554 MW; 5DDF656C8B90F936 CRC64;
MGEALSNLEI ARGAKLLPIE EVGRSMGLRE ERHLEPYGRH VAKVDLCAIE DLSERPKAKY
ILVSAITPTP LGEGKTTTTV GLGQAFSHIG KRATIAIRQA SMGPAFGIKG GAAGGGYSQV
VPMERLNLHL TGDLHAVTEA HNMLAAMIDN HLYHGNGLGI EPHSISWRRV MDVNDRSLRN
IVIGLGARTD GVPRQSGFDI TAASEVMAIL ALASSLEDLR ERLGRIVIGH NREGNPVSAE
DVRGAGAMAV ILKEAIKPNL MQTLEGTPAL VHAGPFGNIA TGNSSVVADL IGIRTADYLI
TEAGFGADMG AERFFNIKCR ISGLEPDAAV VVATVRALKA HSGRYQIKAG APLPEELLEE
NPQDVLAGAE NLKKQIENIK LHGVPAVVAI NAFPTDHPSE HKAIEEAAKE VGARCAVCRH
FTEGGKGAVE LARALEETIE ENERERRRGG GGSFRFLYPL EMPLKQKIET IAREVYGAEG
VEYDAEALRA LEGFERAGFG RLPVCLAKTH LSLSSDPALK GAPRGWKLSV REVRASVGAG
FIYPICGQMR TMPGLSAHPA AERIDLDGEG NVVGLF