ID   FTHS2_LACAC             Reviewed;         553 AA.
AC   Q5FIU5;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Formate--tetrahydrofolate ligase 2 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase 2 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS 2 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS 2 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs2 {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=LBA1562;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000033; AAV43379.1; -; Genomic_DNA.
DR   RefSeq; WP_003548380.1; NC_006814.3.
DR   RefSeq; YP_194410.1; NC_006814.3.
DR   AlphaFoldDB; Q5FIU5; -.
DR   SMR; Q5FIU5; -.
DR   STRING; 272621.LBA1562; -.
DR   PRIDE; Q5FIU5; -.
DR   EnsemblBacteria; AAV43379; AAV43379; LBA1562.
DR   GeneID; 56943130; -.
DR   KEGG; lac:LBA1562; -.
DR   PATRIC; fig|272621.13.peg.1484; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   OMA; CGEIMTM; -.
DR   BioCyc; LACI272621:G1G49-1527-MON; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..553
FT                   /note="Formate--tetrahydrofolate ligase 2"
FT                   /id="PRO_0000199352"
FT   BINDING         63..70
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   553 AA;  59847 MW;  09FF57B54CB4EEF8 CRC64;
     MPTDIEIADA AKMQPITEIA QKLGLSSDDI EQYGHYKAKI NLPVKEVEGK KHKLILVTGI
     NPTSAGEGKS TVLVGLGDAL NQLGHQTTIA MREPSMGPVF GIKGGATGGG YSQVVPMEDI
     NLHFTGDMHA LTSANNTLAA LIDNYIMRDN VLGLDPRRII WKRVEDVNDR VLRNVVTGLG
     GIMQGVPRET GFDITAASEM MAILCLSESL HNLKKRIGRI VVGYTYEKKP VTVDQLGFTG
     AITLLLKDAI KPNLVQTLDH TPAIIHGGPF ANIAHGCNSV LATQIALKTS DYTVTECGFG
     ADLGAEKFLD IKRPVLGETP NAVVIVATAR ALKLNGGADK DELTKEDIPA LEKGMANLRR
     HVENMQKYNL PIVVAINHFN SDTEAEIKTI QDACAEMGVK AIEVDAWAKG GQGTQDLAKE
     VVKLADEELE FHELYKNSDS IEDKLSAIAT KIYGAKRVVL SKKAKRQIKT FNEQGWNDLP
     VCIAKTQYSF TDDQTKLGAP TDFDFHIRDL VPKLGAGFIV ALSGTMMTMP GLAKHPAAEN
     MDIDDNGQIA GLF