ID   FTHS3_RUBXD             Reviewed;         573 AA.
AC   Q1AT39;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Formate--tetrahydrofolate ligase 3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase 3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS 3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS 3 {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs3 {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Rxyl_2519;
OS   Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1).
OC   Bacteria; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae;
OC   Rubrobacter.
OX   NCBI_TaxID=266117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 9941 / NBRC 16129 / PRD-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., da Costa M.S.,
RA   Rainey F.A., Empadinhas N., Jolivet E., Battista J.R., Richardson P.;
RT   "Complete sequence of Rubrobacter xylanophilus DSM 9941.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000386; ABG05439.1; -; Genomic_DNA.
DR   RefSeq; WP_011565449.1; NC_008148.1.
DR   AlphaFoldDB; Q1AT39; -.
DR   SMR; Q1AT39; -.
DR   STRING; 266117.Rxyl_2519; -.
DR   EnsemblBacteria; ABG05439; ABG05439; Rxyl_2519.
DR   KEGG; rxy:Rxyl_2519; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_11; -.
DR   OrthoDB; 177859at2; -.
DR   PhylomeDB; Q1AT39; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000006637; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..573
FT                   /note="Formate--tetrahydrofolate ligase 3"
FT                   /id="PRO_0000293056"
FT   BINDING         66..73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   573 AA;  61311 MW;  CDAE742660E99CAF CRC64;
     MLTNLEIARG AKLLPIEEVG RSMGLREERH LEPYGRHVAK VDLCAIEDLS ERPKAKYILV
     SAITPTPLGE GKTTTTVGLG QAFSHIGKRA TIAIRQASMG PAFGIKGGAA GGGYSQVVPM
     ERLNLHLTGD LHAVTEAHNM LAAMIDNHLY HGNGLGIEPH SISWRRVMDV NDRSLRNIVI
     GLGARTDGVP RQSGFDITAA SEVMAILALA SSLEDLRERL GRIVIGHNRE GNPVSAEDVR
     GAGAMAVILK EAIKPNLMQT LEGTPALVHA GPFGNIATGN SSVVADLIGI RTADYLITEA
     GFGADMGAER FFNIKCRISG LEPDAAVVVA TVRALKAHSG RYQIKAGAPL PEELLEENPQ
     DVLAGAENLK KQIENIKLHG VPAVVAINAF PTDHPSEHKA IEEAAKEVGA RCAVCRHFTE
     GGKGAVELAR ALEETIEENE RERRRGGGGS FRFLYPLEMP LKQKIETIAR EVYGAEGVEY
     DAEALRALEG FERAGFGRLP VCLAKTHLSL SSDPALKGAP RGWKLSVREV RASVGAGFIY
     PICGQMRTMP GLSAHPAAER IDLDGEGNVV GLF