FTHS_ACTP2
ID FTHS_ACTP2 Reviewed; 556 AA.
AC A3MZI4;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2007, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=APL_0466;
OS Actinobacillus pleuropneumoniae serotype 5b (strain L20).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Actinobacillus.
OX NCBI_TaxID=416269;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L20;
RX PubMed=18065534; DOI=10.1128/jb.01845-07;
RA Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M.,
RA Nash J.H.E.;
RT "The complete genome sequence of Actinobacillus pleuropneumoniae L20
RT (serotype 5b).";
RL J. Bacteriol. 190:1495-1496(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000569; ABN73570.1; -; Genomic_DNA.
DR RefSeq; WP_005596596.1; NC_009053.1.
DR AlphaFoldDB; A3MZI4; -.
DR SMR; A3MZI4; -.
DR STRING; 416269.APL_0466; -.
DR EnsemblBacteria; ABN73570; ABN73570; APL_0466.
DR GeneID; 66259331; -.
DR KEGG; apl:APL_0466; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000001432; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..556
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000300513"
FT BINDING 64..71
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 556 AA; 58955 MW; AD5278FBF34F08E5 CRC64;
MKSDVEIAQA ATMQPIHKIA EKLGLNADQI EQYGKYKAKI NPTDAFKLPA KNGKLILVTA
INPTPAGEGK TTVTIGLTDA LNQLGKNAVV AAREPSLGPV FGVKGGAAGG GYAQVLPMED
INLHFTGDFH AITSANNLLA ALLDNHIYQG NALNIDTKRV LWRRVIDMND RQLRNVLGGL
GNPTDGVIRP DGFDITVASE VMAIFCLAKD LADLKTRLGN ILVAYTKDKQ PVYAKDLNAH
GAMAALLKDA IKPNLVQTIE GSPAFIHGGP FANIAHGCNS VTATRLALHL GDYAVTEAGF
GADLGAEKFC DIKCRLADLK PDVAVVVATV KALKYNGGVE KANLAEENLT ALQQGLPNLL
KHISNLKNVF GLPVVVALNR FVSDTDAELA LIQTACAKQG VEVSLTEVWG KGGAGGVDLA
QKVLKAIDEQ ENRFNFVYDV NESVQNKIKA IAQKIYGADD VNFSAEALAE IKNLEKLGLD
KLPICMAKTQ YSLSDNAKLL GCPSGFTVTV RSISVSAGAG FIVAICGSIM RMPGLPKVPA
ANRIDVDENG LITGLF
