ID   FTHS_AERHH              Reviewed;         576 AA.
AC   A0KIN9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=AHA_1604;
OS   Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC
OS   13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=380703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC
RC   2358 / NCIMB 9240 / NCTC 8049;
RX   PubMed=16980456; DOI=10.1128/jb.00621-06;
RA   Seshadri R., Joseph S.W., Chopra A.K., Sha J., Shaw J., Graf J., Haft D.H.,
RA   Wu M., Ren Q., Rosovitz M.J., Madupu R., Tallon L., Kim M., Jin S.,
RA   Vuong H., Stine O.C., Ali A., Horneman A.J., Heidelberg J.F.;
RT   "Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.";
RL   J. Bacteriol. 188:8272-8282(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000462; ABK36840.1; -; Genomic_DNA.
DR   RefSeq; WP_011705499.1; NC_008570.1.
DR   RefSeq; YP_856140.1; NC_008570.1.
DR   AlphaFoldDB; A0KIN9; -.
DR   SMR; A0KIN9; -.
DR   STRING; 380703.AHA_1604; -.
DR   PRIDE; A0KIN9; -.
DR   EnsemblBacteria; ABK36840; ABK36840; AHA_1604.
DR   KEGG; aha:AHA_1604; -.
DR   PATRIC; fig|380703.7.peg.1616; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000756; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..576
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000300514"
FT   BINDING         64..71
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   576 AA;  60860 MW;  BF9395B9F24F6500 CRC64;
     MLSDIEISRQ SPRLPIHALA ERLAIPSHQL HPYGHYKGKL SLELLKSLPK PAGKLVLVSA
     ITPTPLGEGK TVTTLGLSMG LNHIGQPSIA TIRQPSLGPV FGVKGGAAGG GHAQVVPMEE
     MNLHLTGDFH ALTAAHNLAA AALDARLFHE RKLGDQFSAR TGLQRLDIDA DNILWPRTLD
     MNDRALRHLT IGQGNAADGV ERSDRFVITA ASELMAILAL ASDLKDLRQR IGRIQLARDL
     RGKPITAEQL EVAGAMTVLL KEALQPTLMQ TTEQTSVLVH AGPFANIAHG NSSVIADRMA
     LGLTDYVVTE AGFGSDMGLE KFFNIKHRQS GITPACVVLV ATVRGLKANS GLLDIRPGQP
     LPESLLREDL PTLEQGCANL GWHIANARRY GVPVVVAINR FPTDSEAELA LLARAALRAG
     ACGSAISSAF TQGGAGASEL AQAVVAACEQ PGKVKLLYPD QASLGAKLAT LVECGYGGRG
     VQLSDKARQQ LAQLSAEGWD HLPVCVAKTP LSISHDPALK GVPTDFEVPI EEVKLCAGAG
     FVYALAGPIM TMPGLGSLPA YRHIDIDDNG EIVGLS