FTHS_ARATH
ID FTHS_ARATH Reviewed; 634 AA.
AC Q9SPK5;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Formate--tetrahydrofolate ligase;
DE EC=6.3.4.3;
DE AltName: Full=10-formyletrahydrofolate synthetase;
DE Short=FHS;
DE Short=FTHFS;
DE AltName: Full=Formyltetrahydrofolate synthetase;
GN Name=THFS; OrderedLocusNames=At1g50480; ORFNames=F11F12.17, F17J6.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Hypocotyl;
RA Skavdahl M., Olson B.J.S.C., Markwell J., Osterman J.C.;
RT "Nucleotide sequence of a cDNA encoding 10-formyletrahydrofolate synthetase
RT from Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR99-133(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000305}.
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DR EMBL; AF162279; AAD56290.1; -; mRNA.
DR EMBL; AC012561; AAF87882.1; -; Genomic_DNA.
DR EMBL; AC079279; AAG51185.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE32555.1; -; Genomic_DNA.
DR EMBL; AY054637; AAK96828.1; -; mRNA.
DR EMBL; AY081549; AAM10111.1; -; mRNA.
DR PIR; C96541; C96541.
DR RefSeq; NP_564571.1; NM_103931.4.
DR AlphaFoldDB; Q9SPK5; -.
DR SMR; Q9SPK5; -.
DR BioGRID; 26695; 7.
DR IntAct; Q9SPK5; 1.
DR STRING; 3702.AT1G50480.1; -.
DR iPTMnet; Q9SPK5; -.
DR MetOSite; Q9SPK5; -.
DR World-2DPAGE; 0003:Q9SPK5; -.
DR PaxDb; Q9SPK5; -.
DR PRIDE; Q9SPK5; -.
DR ProteomicsDB; 248554; -.
DR EnsemblPlants; AT1G50480.1; AT1G50480.1; AT1G50480.
DR GeneID; 841470; -.
DR Gramene; AT1G50480.1; AT1G50480.1; AT1G50480.
DR KEGG; ath:AT1G50480; -.
DR Araport; AT1G50480; -.
DR TAIR; locus:2008101; AT1G50480.
DR eggNOG; ENOG502QSVW; Eukaryota.
DR HOGENOM; CLU_003601_3_3_1; -.
DR InParanoid; Q9SPK5; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 690393at2759; -.
DR PhylomeDB; Q9SPK5; -.
DR BioCyc; ARA:AT1G50480-MON; -.
DR UniPathway; UPA00193; -.
DR PRO; PR:Q9SPK5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SPK5; baseline and differential.
DR Genevisible; Q9SPK5; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005507; F:copper ion binding; HDA:TAIR.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..634
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199417"
FT BINDING 78..85
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 634 AA; 67802 MW; 5BD9D10603D2FF9A CRC64;
MSSSTRKLEV VSPVPADIDI ANSVEPLHIS EIAKDLNINP LHYDLYGKYK AKVLLSAFDE
LQGQEDGYYV VVGGITPTPL GEGKSTTTVG LCQALGAYLD KKVVTCLRQP SQGPTFGIKG
GAAGGGYSQV IPMDEFNLHL TGDIHAITAS NNLLAAAIDT RIFHETSQSD KALFNRLCPP
NKEGKRSFSD IMFRRLTKLG ISKTSPEELT PEEIKKFARL DIDPASITWR RVMDVNDRFL
RKITIGQGPE EKGMTRETGF DISVASEIMA VLALTTSLGD MRERLGKMVI GNSKAGDPIT
ADDLGVGGAL TVLMKDAINP TLMQTLEGTP VLVHAGPFAN IAHGNSSIVA DKIALKLVGP
GGFVVTEAGF GSDIGTEKFM NIKCRYSGLT PQCAIVVATV RALKMHGGGP DVVAGRPLDR
AYVSENVSLV EAGCVNLAKH ISNTKAYGVN VIVAVNMFAT DTEAELNAVR KFSMDAGAFD
AVVCSHHAHS GKGAVDLGIA VEKACQNITQ PLRFLYPLDI GIKDKIEAIA KSYGASGVEY
SDQAEKQIEM YTQQGFSNLP ICMSKTQYSF SHDASKKGAP SGFVLPIRDV RGSIGAGFIY
PLVGTMSTMP GLPTRPCFYE IDIDTETGKV RGLS
