ID   FTHS_ARTS2              Reviewed;         565 AA.
AC   A0JZ10;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Arth_2901;
OS   Arthrobacter sp. (strain FB24).
OC   Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=290399;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FB24;
RX   PubMed=24501649; DOI=10.4056/sigs.4438185;
RA   Nakatsu C.H., Barabote R., Thompson S., Bruce D., Detter C., Brettin T.,
RA   Han C., Beasley F., Chen W., Konopka A., Xie G.;
RT   "Complete genome sequence of Arthrobacter sp. strain FB24.";
RL   Stand. Genomic Sci. 9:106-116(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000454; ABK04280.1; -; Genomic_DNA.
DR   RefSeq; WP_011692739.1; NC_008541.1.
DR   AlphaFoldDB; A0JZ10; -.
DR   SMR; A0JZ10; -.
DR   STRING; 290399.Arth_2901; -.
DR   PRIDE; A0JZ10; -.
DR   EnsemblBacteria; ABK04280; ABK04280; Arth_2901.
DR   KEGG; art:Arth_2901; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_11; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000754; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..565
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000293032"
FT   BINDING         73..80
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   565 AA;  59652 MW;  2534351725288ABE CRC64;
     MSDNNVMSDL EIAQRATMRP IGDIAAAAGI NADAVELYGR YKAKIDPAKL IDPAGAPKRL
     PGKVVLVSAM SPTPAGEGKS TTTVGLADSL ARAGRNVMIA LREPSLGPVL GMKGGATGGG
     YSQVLPMEEI NLHFTGDFHA ITSANNALMA LVDNHIFQGN ELNIDPRRMT FKRVLDMNDR
     SLREVIIGLG GPAQGVPRQD GFDITVASEI MAVFCLATDI ADLRARLGRI TFGYTYDREP
     VTVADLGVQG ALTMLLRDAI KPNLVQTIAG TPALVHGGPF ANIAHGCNSL IATQTARRLA
     DIVVTEAGFG ADLGAEKFMD IKARVAGVAP SAVVLVATVR ALKMHGGVAK DRLQEPNVEA
     LAAGSANLRR HIRNVEKFGI TPVVAVNKFA TDTPEELDWL LEWCAGEGVQ AAVADVWGRG
     GGGDGGDELA AKVLAALEAP HSFRHLYPLE LSVEDKIRTI VQEMYGADGV DFSVPALKRL
     AEIEKNGWAG MPVCMAKTQY SFSDDATRLG APKGFTVHVR DLIPKTGAGF IVALTGAVMT
     MPGLPKVPAA LRMDVDDTGK PLGLF