FTHS_BACC3
ID FTHS_BACC3 Reviewed; 562 AA.
AC C1ES77;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=BCA_2195;
OS Bacillus cereus (strain 03BB102).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=572264;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=03BB102;
RA Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C.,
RA Tapia R., Han C., Sutton G., Sims D.;
RT "Genome sequence of Bacillus cereus 03BB102.";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP001407; ACO26995.1; -; Genomic_DNA.
DR RefSeq; WP_001985392.1; NC_012472.1.
DR AlphaFoldDB; C1ES77; -.
DR SMR; C1ES77; -.
DR EnsemblBacteria; ACO26995; ACO26995; BCA_2195.
DR KEGG; bcx:BCA_2195; -.
DR OMA; CGEIMTM; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002210; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..562
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000185246"
FT BINDING 71..78
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 562 AA; 60462 MW; 3E8BBBD4C8D76E69 CRC64;
MTTTTTVKSD IEIAQEASMK KIQEIAADLN ILEDELEPYG HYKGKLSLDI FKRLQNEKDG
KVVLVTAINP TPAGEGKSTV TVGLGQAFNK IGKKTVIALR EPSLGPTMGL KGGAAGGGFS
QVVPMEDINL HFTGDIHAIT TANNALAAFI DNHIQQGNTL GIDTRKIVWK RCVDLNDRAL
RNVVIGLGGP VQGVPREDGF DITVASEIMA VFCLATDIQD LKARLSRIVV AYNFANQPVT
VKDLGVEGAL TLLLKDALKP NLVQTLENTP AIIHGGPFAN IAHGCNSVIA TTMAAKLGDY
VITEAGFGAD LGAEKFLDIK ARAAGIKPEA VVIVATIRAL KMHGGVAKDQ LKEENVDALA
KGMENLQKHV ETIQSFGVPF VIAINKFITD TDAEVAYLQE WCNERGYAVS LTEVWEKGGQ
GGVDLAEKVL KEIEKGENNY APLYELELPL EEKIRTIAQK VYGAKDIEFA PKARKQLAQY
EGEGWSNLPI CMAKTQYSLS DDATKLGRPS DFIVTIRELK PSIGAGFIVA LTGTMLTMPG
LPKQPAALQM DVNEDGKAVG LF
