ID   FTHS_BACTN              Reviewed;         555 AA.
AC   Q8A9S8;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=BT_0737;
OS   Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 /
OS   CCUG 10774 / NCTC 10582 / VPI-5482 / E50).
OC   Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC   Bacteroides.
OX   NCBI_TaxID=226186;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 /
RC   VPI-5482 / E50;
RX   PubMed=12663928; DOI=10.1126/science.1080029;
RA   Xu J., Bjursell M.K., Himrod J., Deng S., Carmichael L.K., Chiang H.C.,
RA   Hooper L.V., Gordon J.I.;
RT   "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis.";
RL   Science 299:2074-2076(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE015928; AAO75844.1; -; Genomic_DNA.
DR   RefSeq; NP_809650.1; NC_004663.1.
DR   RefSeq; WP_011107423.1; NC_004663.1.
DR   AlphaFoldDB; Q8A9S8; -.
DR   SMR; Q8A9S8; -.
DR   STRING; 226186.BT_0737; -.
DR   PaxDb; Q8A9S8; -.
DR   PRIDE; Q8A9S8; -.
DR   EnsemblBacteria; AAO75844; AAO75844; BT_0737.
DR   GeneID; 60926705; -.
DR   KEGG; bth:BT_0737; -.
DR   PATRIC; fig|226186.12.peg.752; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_10; -.
DR   InParanoid; Q8A9S8; -.
DR   OMA; CGEIMTM; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001414; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..555
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199335"
FT   BINDING         64..71
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   555 AA;  60482 MW;  2E9B6E45357D0F8D CRC64;
     MKSDIEIARS VELKKIKQVA ESIGIPREEV ENYGRYIAKI PEQLIDEEKV KKSNLVLVTA
     ITATKAGIGK TTVSIGLALG LNKIGKKAIV ALREPSLGPC FGMKGGAAGG GYAQVLPMEK
     INLHFTGDFH AITSAHNMIS ALLDNYLYQN QAKGFGLKEI LWRRVLDVND RSLRSIVVGL
     GPKSNGITQE SGFDITPASE IMAILCLSKD VEDLRRRIEN ILLGFTYDDQ PFTVKDLGVA
     GAITVLLKDA IHPNLVQTTE GTAAFVHGGP FANIAHGCNS ILATKLAMSF GDYVITEAGF
     GADLGAEKFY NIKCRKSGLQ PKLTVIVATA QGLKMHGGVS LDRIKEPNME GLKEGLRNLD
     KHIRNLRSFG QTVVVAFNKF ATDTDEEMEM LREHCEQLGV GYAINNAFSD GGDGAVDMAR
     LVVDTIENNP SEPLRYTYKE EDSIQQKIEK VATNLYGASV ITYSSIARNR IKLIEKMGIT
     HYPVCIAKTQ YSFSADPKIY GAVNNFEFHI KDIVINNGAE MIVAIAGEIL RMPGLPKEPQ
     ALHIDIVDGE IEGLS