ID   FTHS_BIFLO              Reviewed;         505 AA.
AC   Q8G700;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Formate--tetrahydrofolate ligase;
DE            EC=6.3.4.3;
DE   AltName: Full=Formyltetrahydrofolate synthetase;
DE            Short=FHS;
DE            Short=FTHFS;
GN   Name=fhs; OrderedLocusNames=BL0478;
OS   Bifidobacterium longum (strain NCC 2705).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=206672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCC 2705;
RX   PubMed=12381787; DOI=10.1073/pnas.212527599;
RA   Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G.,
RA   Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.;
RT   "The genome sequence of Bifidobacterium longum reflects its adaptation to
RT   the human gastrointestinal tract.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000305}.
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DR   EMBL; AE014295; AAN24309.1; -; Genomic_DNA.
DR   RefSeq; NP_695673.1; NC_004307.2.
DR   RefSeq; WP_011068506.1; NC_004307.2.
DR   AlphaFoldDB; Q8G700; -.
DR   SMR; Q8G700; -.
DR   STRING; 206672.BL0478; -.
DR   EnsemblBacteria; AAN24309; AAN24309; BL0478.
DR   KEGG; blo:BL0478; -.
DR   PATRIC; fig|206672.9.peg.1221; -.
DR   HOGENOM; CLU_030416_0_0_11; -.
DR   OMA; WDGEDDQ; -.
DR   PhylomeDB; Q8G700; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000439; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..505
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199336"
SQ   SEQUENCE   505 AA;  54028 MW;  B5643626B9884BC8 CRC64;
     MTTIEDFTSQ YGLVQKIDAF GYMKYLAENP DAPRKHGKVV LVTADTPLKA SRGEGKTTTT
     IALIDALNKR GIDATAVLRQ PSMGITAAGS KGGASGGGKA SLTHPELIDW GLCGEMGAIE
     AAQNLLVSFA EKAIDDGKLD TILVPRVSEV PSRSLRSTAV DYGKGNVPEK VVLTPTCELM
     QIVVLSRSMD EISERVSKMI AGTKDGKAVT FGEFVDLWRI TGILADAVKP AKTETVNGSP
     VYVHGGPFAN VSIGIPTLVS VEMACALHDV VIVEAGYGTD AGAQKWLDIA CREYGAQWPS
     AAIVVTRAST WRDDPELAWR YPFHVQRLEN LDIPTFPLIN LWEGEDDQVP SLKATADELG
     FRTPIIGNLF RDGGEALAPQ LDGFVDALQN GSMPAEPHSH KGMALTENAK WVAENAYGVP
     AERVIYKPGF TESVSEAMEL CQSAGISLDD LAFVAVKSPA TMTDNDRAPE EERTVALKKV
     EVHAGAGLVH VNLTTSLTTP MPKIV