ID   FTHS_CLOBB              Reviewed;         556 AA.
AC   B2TI29;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=CLL_A0173;
OS   Clostridium botulinum (strain Eklund 17B / Type B).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=935198;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Eklund 17B / Type B;
RA   Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Complete sequence of Clostridium botulinum strain Eklund.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP001056; ACD22749.1; -; Genomic_DNA.
DR   RefSeq; WP_012423596.1; NC_018648.1.
DR   AlphaFoldDB; B2TI29; -.
DR   SMR; B2TI29; -.
DR   EnsemblBacteria; ACD22749; ACD22749; CLL_A0173.
DR   KEGG; cbk:CLL_A0173; -.
DR   PATRIC; fig|935198.13.peg.157; -.
DR   HOGENOM; CLU_003601_3_3_9; -.
DR   OMA; CGEIMTM; -.
DR   OrthoDB; 177859at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000001195; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..556
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000146677"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   556 AA;  60252 MW;  93134614A9466369 CRC64;
     MKTDIEIAQE AEMLHIKNVA EKLGLDEENI EYYGKYKCKV SLDVYNKVKN NRDGKLVLVT
     AINPTPAGEG KSTVTVGLGD ALNKMGKNTV IALREPSLGP VFGIKGGAAG GGYAQVVPME
     DINLHFTGDM HAITSANNLL SAAIDNHIHQ GNNLRIDSRR IIFKRVMDMN DRALRKIIVG
     MGGKINGFVR EDGFTITVAS EIMAILCLAS DLEDLKHRMG DILIAYDLDG NPVYAKQLEI
     QGAMALLMKD AIKPNLVQTL ENTPALIHGG PFANIAHGCN SIMATKLSLK LGDIVVTEAG
     FGADLGAEKF FDIKCRYGNL KPCCVVIVAT IRALKHHGGV AKADLNTPNV EALRLGIANL
     EKQIENIKKF NVEPVVAINK FVSDSDEEVE FIKEFCEKLG VKVALSDVWA KGGDGGIELG
     EAVLDVIEKD KSNFKTLYDT DKTIEEKILT IAKEIYGADG VVYSNEAKKQ IGELVKFNLD
     KLPICMAKTQ YSLSDNPNLL AKPSGFNINV QEIRVSNGAG FIVVQTGNIM TMPGLPKVPA
     ANKMDVLKDG EIVGLF