FTHS_CLOCY
ID FTHS_CLOCY Reviewed; 556 AA.
AC Q07064;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
OS Clostridium cylindrosporum.
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1495;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8443597; DOI=10.1002/pro.5560020208;
RA Rankin C.A., Haslam G.C., Himes R.H.;
RT "Sequence and expression of the gene for N10-formyltetrahydrofolate
RT synthetase from Clostridium cylindrosporum.";
RL Protein Sci. 2:197-205(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; L12465; AAA23239.1; -; Genomic_DNA.
DR PIR; I40808; I40808.
DR AlphaFoldDB; Q07064; -.
DR SMR; Q07064; -.
DR BioCyc; MetaCyc:MON-13114; -.
DR UniPathway; UPA00193; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..556
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_0000199339"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 556 AA; 59096 MW; 44C16B0A957890FB CRC64;
MKTDVQIAQE AQMKPITEVA NYLGIQDDEL ELYGKYKAKV SLDVLERQKD KEDAKLVLVT
AINPTPAGEG KTTTNVGLSM GLNKIGKRTI TALREPSLGP CFGVKGGAAG GGYAQVVPMD
DINLHFTGDF HAITSAHNLL AALLDNHLHQ GNALNINPKK IVWKRVIDMN DRSLRNVIIG
LGGNGDGFVR QAQFDITVAS EIMAILCLAT SMSDLKERLS KMIVAYAKDG SAVTAGQLEA
TGAMALLLKD AVKPNLVQTL ENTPAFIHGG PFANIAHGCN SVLATKVALK LADYVVTEGG
FGADLGAEKF FDIKSRFAGL KPNCDVSVAT VRALKMNGGV PKTELAAENV EAVKKGVANL
ERHIENVAKF GVPAVVAINK FPLDTEAELK AVEDACNAKG ADVVLSDVWA NGGEGGVEMA
KKVVEICEKN EANFAPLYDV NLSIPEKIEK IATTIYRADG VDFTSDCKKQ IAELEKLGLD
KMPICMAKTQ YSFSDDPTLL GAPTGFRITV REVRVSAGAG FIVALTGNMM TMPGLPKVPA
ANGMDILESG EIIGLS